##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI BbeI BbrPI BbvCI
BciVI BclI BglII BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BspEI BsrFI BssHII BstEII
BstPI CelII Cfr10I CpoI DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoT22I
FbaI FseI HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SbfI SexAI SfoI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2879 GACGTC GACGT^C
AflII 3395 CTTAAG C^TTAAG
AflIII 1676 ACATGT A^C[GA][CT]GT
AocI 1557 CCTGAGG CC^T[ACGT]AGG
ApaI 82 GGGCCC GGGCC^C
AseI 723 ATTAAT AT^TAAT
AsnI 723 ATTAAT AT^TAAT
AviII 295 TGCGCA TGC^GCA
AvrII 3302 CCTAGG C^CTAGG
BanI 2381 GGCACC G^G[CT][GA]CC
BfrI 3395 CTTAAG C^TTAAG
BlnI 3302 CCTAGG C^CTAGG
Bsp1407I 1080 TGTACA T^GTACA
BspDI 1894 ATCGAT AT^CGAT
BspHI 2911 TCATGA T^CATGA
BspLU11I 1676 ACATGT A^CATGT
BsrBI 2492 CCGCTC CCGCTC(-3/-3)
BsrGI 1080 TGTACA T^GTACA
BstAPI 3132 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2740 TTCGAA TT^CGAA
BstXI 518 CCATAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1557 CCTGAGG CC^T[ACGT]AGG
ClaI 1894 ATCGAT AT^CGAT
Eco81I 1557 CCTGAGG CC^T[ACGT]AGG
EcoRI 496 GAATTC G^AATTC
EcoRV 1005 GATATC GAT^ATC
FspI 295 TGCGCA TGC^GCA
HaeII 2694 AGCGCC [GA]GCGC^[CT]
NcoI 2194 CCATGG C^CATGG
NheI 130 GCTAGC G^CTAGC
NotI 193 GCGGCCGC GC^GGCCGC
NruI 2363 TCGCGA TCG^CGA
NspI 1676 ACATGT [GA]CATG^[CT]
NspV 2740 TTCGAA TT^CGAA
PaeR7I 2113 CTCGAG C^TCGAG
PflMI 2176 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 723 ATTAAT AT^TAAT
PvuII 1219 CAGCTG CAG^CTG
RcaI 2911 TCATGA T^CATGA
SalI 2041 GTCGAC G^TCGAC
SfiI 186 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2740 TTCGAA TT^CGAA
SpeI 3338 ACTAGT A^CTAGT
SspBI 1080 TGTACA T^GTACA
Van91I 2176 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2349 TCTAGA T^CTAGA
XhoI 2113 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 2879 GACGTC G[GA]^CG[CT]C
3290 GACGCC
AspHI 2915 GAGCTC G[AT]GC[AT]^C
3229 GAGCTC
AvaI 1105 CTCGGG C^[CT]CG[GA]G
2113 CTCGAG
BcgI 1598 CGAAACTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
298 GCATCGTCATCG
BpmI 2884 CTCCAG CTGGAG(16/14)
1143 CTGGAG
BsaHI 2879 GACGTC G[GA]^CG[CT]C
3290 GACGCC
BsgI 549 CTGCAC GTGCAG(16/14)
2893 GTGCAG
BsiEI 175 CGGCCG CG[GA][CT]^CG
194 CGGCCG
BsiHKAI 2915 GAGCTC G[AT]GC[AT]^C
3229 GAGCTC
BsmBI 591 CGTCTC CGTCTC(1/5)
2881 CGTCTC
BsmI 311 GAATGC GAATGC(1/-1)
1879 GCATTC
BsoBI 1105 CTCGGG C^[CT]CG[GA]G
2113 CTCGAG
BssSI 2648 CACGAG CACGAG(-5/-1)
1950 CTCGTG
Bst1107I 2103 GTATAC GTA^TAC
3385 GTATAC
BstZ17I 2103 GTATAC GTA^TAC
3385 GTATAC
DraI 536 TTTAAA TTT^AAA
845 TTTAAA
DraIII 2177 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2518 CACAGAGTG
EagI 175 CGGCCG C^GGCCG
194 CGGCCG
EclXI 175 CGGCCG C^GGCCG
194 CGGCCG
Eco52I 175 CGGCCG C^GGCCG
194 CGGCCG
EcoT14I 2194 CCATGG C^C[AT][AT]GG
3302 CCTAGG
Hin1I 2879 GACGTC G[GA]^CG[CT]C
3290 GACGCC
HincII 2041 GTCGAC GT[CT]^[GA]AC
2334 GTTGAC
HindII 2041 GTCGAC GT[CT]^[GA]AC
2334 GTTGAC
HindIII 1720 AAGCTT A^AGCTT
1996 AAGCTT
HphI 1188 GGTGA GGTGA(8/7)
1499 GGTGA
KspI 125 CCGCGG CCGC^GG
188 CCGCGG
MaeII 229 ACGT A^CGT
2880 ACGT
SacI 2915 GAGCTC GAGCT^C
3229 GAGCTC
SacII 125 CCGCGG CCGC^GG
188 CCGCGG
SapI 615 GCTCTTC GCTCTTC(1/4)
1339 GCTCTTC
ScaI 1665 AGTACT AGT^ACT
3335 AGTACT
StyI 2194 CCATGG C^C[AT][AT]GG
3302 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AvaII BalI BamHI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI