##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Alw44I AlwI AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BbrPI BbvCI
BclI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrDI
BssHII BstBI BstEII BstPI BstYI
ClaI CpoI DpnII DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HindIII KpnI KspI MamI MboI
MfeI MflI MluI MroI MunI
NaeI NdeII NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI Sau3AI SbfI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SwaI TaqI TthHB8I
Van91I XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 187 GTATAC GT^[AC][GT]AC
AcsI 2337 AAATTT [GA]^AATT[CT]
AgeI 2697 ACCGGT A^CCGGT
AhdI 549 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1057 CCTCAGG CC^T[ACGT]AGG
ApoI 2337 AAATTT [GA]^AATT[CT]
AspEI 549 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2216 CGAGGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 702 GCCCATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 438 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 438 GCTCAGC GC^T[ACGT]AGC
BsaI 2067 GGTCTC GGTCTC(1/5)
BsaWI 2697 ACCGGT [AT]^CCGG[AT]
Bsp1407I 1566 TGTACA T^GTACA
BspMI 1942 GCAGGT ACCTGC(4/8)
BsrBI 309 CCGCTC CCGCTC(-3/-3)
BsrFI 2697 ACCGGT [GA]^CCGG[CT]
BsrGI 1566 TGTACA T^GTACA
BssSI 2125 CACGAG CACGAG(-5/-1)
Bst1107I 187 GTATAC GTA^TAC
BstAPI 165 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 387 CCAGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 187 GTATAC GTA^TAC
Bsu36I 1057 CCTCAGG CC^T[ACGT]AGG
CelII 438 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2697 ACCGGT [GA]^CCGG[CT]
Eam1105I 549 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2195 CTCTTC CTCTTC(1/4)
Eco81I 1057 CCTCAGG CC^T[ACGT]AGG
EcoNI 856 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1413 ATGCAT ATGCA^T
HgaI 919 GACGC GACGC(5/10)
HpaI 724 GTTAAC GTT^AAC
Ksp632I 2195 CTCTTC CTCTTC(1/4)
NdeI 406 CATATG CA^TATG
NsiI 1413 ATGCAT ATGCA^T
PinAI 2697 ACCGGT A^CCGGT
PvuII 1489 CAGCTG CAG^CTG
SacI 2034 GAGCTC GAGCT^C
ScaI 800 AGTACT AGT^ACT
SexAI 182 ACCTGGT A^CC[AT]GGT
SmaI 975 CCCGGG CCC^GGG
SphI 527 GCATGC GCATG^C
SspBI 1566 TGTACA T^GTACA
TfiI 1652 GAATC G^A[AT]TC
XbaI 1388 TCTAGA T^CTAGA
XmaCI 975 CCCGGG C^CCGGG
XmaI 975 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 1505 CGCG CG^CG
2214 CGCG
AcyI 699 GGCGCC G[GA]^CG[CT]C
2178 GGCGCC
AflIII 11 ACATGT A^C[GA][CT]GT
2367 ACATGT
ApaI 348 GGGCCC GGGCC^C
786 GGGCCC
AspI 428 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
848 GACAAGGTC
AvaI 33 CCCGAG C^[CT]CG[GA]G
975 CCCGGG
BalI 906 TGGCCA TGG^CCA
2233 TGGCCA
BanI 699 GGCGCC G^G[CT][GA]CC
2178 GGCGCC
BbeI 699 GGCGCC GGCGC^C
2178 GGCGCC
BcnI 975 CCCGG CC^[GC]GG
976 CCGGG
BsaHI 699 GGCGCC G[GA]^CG[CT]C
2178 GGCGCC
BsoBI 33 CCCGAG C^[CT]CG[GA]G
975 CCCGGG
BspLU11I 11 ACATGT A^CATGT
2367 ACATGT
BstUI 1505 CGCG CG^CG
2214 CGCG
HapII 976 CCGG C^CGG
2698 CCGG
Hin1I 699 GGCGCC G[GA]^CG[CT]C
2178 GGCGCC
HincII 724 GTTAAC GT[CT]^[GA]AC
2578 GTCAAC
HindII 724 GTTAAC GT[CT]^[GA]AC
2578 GTCAAC
HpaII 976 CCGG C^CGG
2698 CCGG
KasI 699 GGCGCC G^GCGCC
2178 GGCGCC
MluNI 906 TGGCCA TGG^CCA
2233 TGGCCA
MscI 906 TGGCCA TGG^CCA
2233 TGGCCA
MspI 976 CCGG C^CGG
2698 CCGG
MvnI 1505 CGCG CG^CG
2214 CGCG
NarI 699 GGCGCC GG^CGCC
2178 GGCGCC
NciI 975 CCCGG CC^[GC]GG
976 CCGGG
PflFI 428 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
848 GACAAGGTC
SfcI 768 CTACAG C^T[GA][CT]AG
1086 CTATAG
SfoI 699 GGCGCC GGC^GCC
2178 GGCGCC
SspI 878 AATATT AAT^ATT
1586 AATATT
StuI 140 AGGCCT AGG^CCT
1129 AGGCCT
Tth111I 428 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
848 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraI DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
HphI ItaI MaeI MaeII MaeIII
MboII MnlI MseI MslI MspA1I
MvaI MwoI NcoI NlaIII NlaIV
NspI PleI PpuMI RsaI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI