##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AccII AclI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AviII AvrII BalI BbeI
BbrPI BciVI BfrI BglI BlnI
BsiEI BsiWI Bsp1407I BspDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI KasI KspI MluI
MluNI MscI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 191 GACGTC GACGT^C
Acc65I 3113 GGTACC G^GTACC
AflIII 1174 ACATGT A^C[GA][CT]GT
Alw44I 3071 GTGCAC G^TGCAC
ApaLI 3071 GTGCAC G^TGCAC
Asp700I 1846 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3113 GGTACC G^GTACC
AspI 812 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 85 CTCGAG C^[CT]CG[GA]G
BamHI 26 GGATCC G^GATCC
BbvCI 2300 GCTGAGG CCTCAGC(-5/-2)
BclI 1578 TGATCA T^GATCA
BcnI 1652 CCCGG CC^[GC]GG
BglII 1510 AGATCT A^GATCT
BlpI 461 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 461 GCTGAGC GC^T[ACGT]AGC
BsaAI 2045 CACGTA [CT]AC^GT[GA]
BsaBI 1208 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1726 GAATGC GAATGC(1/-1)
BsoBI 85 CTCGAG C^[CT]CG[GA]G
BspHI 2400 TCATGA T^CATGA
BspLU11I 1174 ACATGT A^CATGT
BssSI 233 CACGAG CACGAG(-5/-1)
BstXI 684 CCAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 461 GCTGAGC GC^T[ACGT]AGC
DrdI 3003 GACCAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2202 CCGTGG C^C[GA][CT]GG
EcoRI 2380 GAATTC G^AATTC
EcoT14I 940 CCAAGG C^C[AT][AT]GG
FbaI 1578 TGATCA T^GATCA
HaeII 1193 GGCGCT [GA]GCGC^[CT]
HpaI 2913 GTTAAC GTT^AAC
KpnI 3113 GGTACC GGTAC^C
MamI 1208 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1060 CAATTG C^AATTG
MunI 1060 CAATTG C^AATTG
NciI 1652 CCCGG CC^[GC]GG
PaeR7I 85 CTCGAG C^TCGAG
PflFI 812 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 482 CCACATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2400 TCATGA T^CATGA
SapI 1003 GAAGAGC GCTCTTC(1/4)
SpeI 2450 ACTAGT A^CTAGT
StyI 940 CCAAGG C^C[AT][AT]GG
Tth111I 812 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 482 CCACATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 85 CTCGAG C^TCGAG
XmnI 1846 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2690 GTAGAC GT^[AC][GT]AC
3000 GTAGAC
AccIII 1635 TCCGGA T^CCGGA
1777 TCCGGA
AocI 1922 CCTGAGG CC^T[ACGT]AGG
2895 CCTGAGG
BanI 2040 GGTGCC G^G[CT][GA]CC
3113 GGTACC
BcgI 3129 CGAGGATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2002 GCATGTTGATCG
BsaWI 1635 TCCGGA [AT]^CCGG[AT]
1777 TCCGGA
BseAI 1635 TCCGGA T^CCGGA
1777 TCCGGA
BsmBI 1496 GAGACG CGTCTC(1/5)
2671 GAGACG
BspEI 1635 TCCGGA T^CCGGA
1777 TCCGGA
BstAPI 628 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1750 GCAGGAAGTGC
BstYI 26 GGATCC [GA]^GATC[CT]
1510 AGATCT
Bsu36I 1922 CCTGAGG CC^T[ACGT]AGG
2895 CCTGAGG
CfoI 422 GCGC GCG^C
1194 GCGC
DraII 1941 GGGACCT [GA]G^G[ACGT]CC[CT]
3016 AGGACCT
Eco81I 1922 CCTGAGG CC^T[ACGT]AGG
2895 CCTGAGG
EcoO109I 1941 GGGACCT [GA]G^G[ACGT]CC[CT]
3016 AGGACCT
HhaI 422 GCGC GCG^C
1194 GCGC
HinP1I 422 GCGC G^CGC
1194 GCGC
HindIII 916 AAGCTT A^AGCTT
3231 AAGCTT
MflI 26 GGATCC [GA]^GATC[CT]
1510 AGATCT
MroI 1635 TCCGGA T^CCGGA
1777 TCCGGA
NspI 1174 ACATGT [GA]CATG^[CT]
2002 GCATGT
PpuMI 1941 GGGACCT [GA]G^G[AT]CC[CT]
3016 AGGACCT
PstI 1935 CTGCAG CTGCA^G
2220 CTGCAG
PvuII 2479 CAGCTG CAG^CTG
2918 CAGCTG
SexAI 1011 ACCAGGT A^CC[AT]GGT
2391 ACCAGGT
XcmI 513 CCAAAGAATATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
985 CCAGCCCCCGTGTGG
XhoII 26 GGATCC [GA]^GATC[CT]
1510 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
DraIII EarI EcoNI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I