##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
BbeI BclI BfrI BlpI Bpu1102I
BsaHI BsiWI BssHII Bst1107I BstBI
BstZ17I CelII CpoI DrdI Eam1105I
Eco47III EcoRI FbaI FseI FspI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MunI NarI NdeI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1313 GGTACC G^GTACC
AccI 3571 GTCTAC GT^[AC][GT]AC
AccII 1891 CGCG CG^CG
AccIII 665 TCCGGA T^CCGGA
AcsI 3644 AAATTT [GA]^AATT[CT]
ApaI 132 GGGCCC GGGCC^C
ApoI 3644 AAATTT [GA]^AATT[CT]
Asp718I 1313 GGTACC G^GTACC
AvrII 1985 CCTAGG C^CTAGG
BcgI 1863 CGAGTTTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1985 CCTAGG C^CTAGG
BsaBI 3474 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 665 TCCGGA T^CCGGA
BsiEI 155 CGGCCG CG[GA][CT]^CG
Bsp1407I 2290 TGTACA T^GTACA
BspDI 490 ATCGAT AT^CGAT
BspEI 665 TCCGGA T^CCGGA
BspHI 1934 TCATGA T^CATGA
BsrDI 1460 GCAATG GCAATG(2/0)
BsrFI 1226 GCCGGC [GA]^CCGG[CT]
BsrGI 2290 TGTACA T^GTACA
BssSI 1861 CACGAG CACGAG(-5/-1)
BstEII 1701 GGTCACC G^GT[ACGT]ACC
BstPI 1701 GGTCACC G^GT[ACGT]ACC
BstUI 1891 CGCG CG^CG
Cfr10I 1226 GCCGGC [GA]^CCGG[CT]
ClaI 490 ATCGAT AT^CGAT
DraI 2562 TTTAAA TTT^AAA
EagI 155 CGGCCG C^GGCCG
EclXI 155 CGGCCG C^GGCCG
Eco52I 155 CGGCCG C^GGCCG
EcoO65I 1701 GGTCACC G^GT[ACGT]ACC
EcoRV 1513 GATATC GAT^ATC
EcoT22I 1182 ATGCAT ATGCA^T
HaeII 2044 AGCGCC [GA]GCGC^[CT]
HincII 1048 GTTGAC GT[CT]^[GA]AC
HindII 1048 GTTGAC GT[CT]^[GA]AC
KpnI 1313 GGTACC GGTAC^C
MamI 3474 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 665 TCCGGA T^CCGGA
MvnI 1891 CGCG CG^CG
NaeI 1226 GCCGGC GCC^GGC
NgoMIV 1226 GCCGGC G^CCGGC
NheI 1776 GCTAGC G^CTAGC
NotI 154 GCGGCCGC GC^GGCCGC
NsiI 1182 ATGCAT ATGCA^T
PshAI 413 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1934 TCATGA T^CATGA
SacI 2224 GAGCTC GAGCT^C
SfiI 156 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 128 CCCGGG CCC^GGG
SmlI 399 CTCAAG C^T[CT][GA]AG
SpeI 1341 ACTAGT A^CTAGT
SspBI 2290 TGTACA T^GTACA
TfiI 849 GAATC G^A[AT]TC
XmaCI 128 CCCGGG C^CCGGG
XmaI 128 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspI 1102 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3272 GACAGGGTC
AvaI 128 CCCGGG C^[CT]CG[GA]G
135 CCCGAG
BamHI 1252 GGATCC G^GATCC
1536 GGATCC
BanI 1313 GGTACC G^G[CT][GA]CC
2905 GGTGCC
BbrPI 826 CACGTG CAC^GTG
2241 CACGTG
BciVI 1665 GGATAC GTATCC(6/5)
1321 GTATCC
BglI 157 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1906 GCCATGTGGGC
BglII 2140 AGATCT A^GATCT
3218 AGATCT
BsaAI 826 CACGTG [CT]AC^GT[GA]
2241 CACGTG
BsmBI 2129 CGTCTC CGTCTC(1/5)
232 GAGACG
BsmI 1180 GAATGC GAATGC(1/-1)
3106 GAATGC
BsoBI 128 CCCGGG C^[CT]CG[GA]G
135 CCCGAG
BspLU11I 644 ACATGT A^CATGT
2050 ACATGT
BsrBI 37 GAGCGG CCGCTC(-3/-3)
152 GAGCGG
BstAPI 776 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1888 GCACGCGCTGC
BstXI 900 CCAGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1860 CCACGAGTTTGG
CfoI 1892 GCGC GCG^C
2045 GCGC
DraIII 2854 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2872 CACAGTGTG
FauI 57 GCGGG CCCGC(4/6)
121 GCGGG
HgaI 3526 GACGC GACGC(5/10)
2128 GCGTC
HhaI 1892 GCGC GCG^C
2045 GCGC
HinP1I 1892 GCGC G^CGC
2045 GCGC
PflFI 1102 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3272 GACAGGGTC
PflMI 1617 CCATAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2853 CCACACTGTGG
PmaCI 826 CACGTG CAC^GTG
2241 CACGTG
PmlI 826 CACGTG CAC^GTG
2241 CACGTG
PpuMI 2487 AGGTCCC [GA]G^G[AT]CC[CT]
2949 GGGTCCT
SexAI 1295 ACCAGGT A^CC[AT]GGT
1316 ACCAGGT
Tth111I 1102 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3272 GACAGGGTC
Van91I 1617 CCATAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2853 CCACACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI AspHI AvaII BalI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII