################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccI AflII Aor51HI ApaI AscI Asp700I Asp718I AspI AviII BcgI BciVI BclI BfrI BlpI Bpu1102I BsaBI BsiWI BsmBI BspDI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII DrdI Eco47III EcoO65I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MunI NaeI NdeI NgoMIV NheI NruI NspV PaeR7I PflFI PmeI PshAI PvuI RsrII SacI SalI ScaI SfuI SgrAI SnaBI SpeI SphI StuI SwaI Tth111I XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 71 enzymes) # ################################### AccIII 1492 TCCGGA T^CCGGA AclI 3366 AACGTT AA^CGTT AcyI 3652 GGCGCC G[GA]^CG[CT]C AgeI 2229 ACCGGT A^CCGGT AhdI 2228 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2293 GTGCAC G^TGCAC AlwNI 2040 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 772 CCTGAGG CC^T[ACGT]AGG ApaLI 2293 GTGCAC G^TGCAC AspEI 2228 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 55 CCCGGG C^[CT]CG[GA]G AvrII 2275 CCTAGG C^CTAGG BalI 1908 TGGCCA TGG^CCA BamHI 174 GGATCC G^GATCC BbeI 3652 GGCGCC GGCGC^C BbrPI 2443 CACGTG CAC^GTG BbvCI 1093 CCTCAGC CCTCAGC(-5/-2) BlnI 2275 CCTAGG C^CTAGG BpmI 78 CTGGAG CTGGAG(16/14) BsaAI 2443 CACGTG [CT]AC^GT[GA] BsaHI 3652 GGCGCC G[GA]^CG[CT]C BseAI 1492 TCCGGA T^CCGGA BsiEI 208 CGGCCG CG[GA][CT]^CG BsmI 438 GAATGC GAATGC(1/-1) BsoBI 55 CCCGGG C^[CT]CG[GA]G BspEI 1492 TCCGGA T^CCGGA BsrFI 2229 ACCGGT [GA]^CCGG[CT] BstAPI 3108 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 772 CCTGAGG CC^T[ACGT]AGG Cfr10I 2229 ACCGGT [GA]^CCGG[CT] DraII 2271 GGGACCT [GA]G^G[ACGT]CC[CT] EagI 208 CGGCCG C^GGCCG Eam1105I 2228 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 208 CGGCCG C^GGCCG Eco52I 208 CGGCCG C^GGCCG Eco81I 772 CCTGAGG CC^T[ACGT]AGG EcoNI 714 CCTATTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2271 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 674 GAATTC G^AATTC EcoRV 2459 GATATC GAT^ATC EcoT22I 3377 ATGCAT ATGCA^T HgaI 785 GACGC GACGC(5/10) Hin1I 3652 GGCGCC G[GA]^CG[CT]C KasI 3652 GGCGCC G^GCGCC KspI 46 CCGCGG CCGC^GG MluI 816 ACGCGT A^CGCGT MluNI 1908 TGGCCA TGG^CCA MroI 1492 TCCGGA T^CCGGA MscI 1908 TGGCCA TGG^CCA NarI 3652 GGCGCC GG^CGCC NcoI 2336 CCATGG C^CATGG NotI 207 GCGGCCGC GC^GGCCGC NsiI 3377 ATGCAT ATGCA^T PacI 923 TTAATTAA TTAAT^TAA PinAI 2229 ACCGGT A^CCGGT PmaCI 2443 CACGTG CAC^GTG PmlI 2443 CACGTG CAC^GTG PpuMI 2271 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 3366 AACGTT AA^CGTT SacII 46 CCGCGG CCGC^GG SapI 3594 GAAGAGC GCTCTTC(1/4) SbfI 883 CCTGCAGG CCTGCA^GG SfiI 111 GGCCGTGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3652 GGCGCC GGC^GCC SmaI 55 CCCGGG CCC^GGG Sse8387I 883 CCTGCAGG CCTGCA^GG TaqI 2845 TCGA T^CGA TthHB8I 2845 TCGA T^CGA XbaI 3221 TCTAGA T^CTAGA XmaCI 55 CCCGGG C^CCGGG XmaI 55 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 39 GGTCC G^G[AT]CC 2272 GGACC BanII 60 GAGCCC G[GA]GC[CT]^C 1789 GAGCCC BglI 112 GCCGTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 126 GCCCAGGAGGC BsaI 1259 GGTCTC GGTCTC(1/5) 2235 GGTCTC Bsp1407I 766 TGTACA T^GTACA 2697 TGTACA BspHI 306 TCATGA T^CATGA 732 TCATGA BspLU11I 704 ACATGT A^CATGT 763 ACATGT BspMI 3389 ACCTGC ACCTGC(4/8) 886 GCAGGT BsrGI 766 TGTACA T^GTACA 2697 TGTACA BstXI 3011 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3238 CCAGGCAGCTGG DsaI 46 CCGCGG C^C[GA][CT]GG 2336 CCATGG EaeI 208 CGGCCG [CT]^GGCC[GA] 1908 TGGCCA EcoT14I 2275 CCTAGG C^C[AT][AT]GG 2336 CCATGG HaeII 184 AGCGCC [GA]GCGC^[CT] 3652 GGCGCC PflMI 1146 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2876 CCAAGTGCTGG RcaI 306 TCATGA T^CATGA 732 TCATGA SmlI 561 CTCAAG C^T[CT][GA]AG 2430 CTTGAG SspBI 766 TGTACA T^GTACA 2697 TGTACA SspI 918 AATATT AAT^ATT 2548 AATATT StyI 2275 CCTAGG C^C[AT][AT]GG 2336 CCATGG Van91I 1146 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2876 CCAAGTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI AspHI BanI BbsI BbvI BcnI BfaI BglII BmyI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII