##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AflII Aor51HI
ApaI AscI Asp700I Asp718I AspI
AviII BcgI BciVI BclI BfrI
BlpI Bpu1102I BsaBI BsiWI BsmBI
BspDI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII DrdI Eco47III
EcoO65I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MunI NaeI NdeI NgoMIV
NheI NruI NspV PaeR7I PflFI
PmeI PshAI PvuI RsrII SacI
SalI ScaI SfuI SgrAI SnaBI
SpeI SphI StuI SwaI Tth111I
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AccIII 1492 TCCGGA T^CCGGA
AclI 3366 AACGTT AA^CGTT
AcyI 3652 GGCGCC G[GA]^CG[CT]C
AgeI 2229 ACCGGT A^CCGGT
AhdI 2228 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2293 GTGCAC G^TGCAC
AlwNI 2040 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 772 CCTGAGG CC^T[ACGT]AGG
ApaLI 2293 GTGCAC G^TGCAC
AspEI 2228 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 55 CCCGGG C^[CT]CG[GA]G
AvrII 2275 CCTAGG C^CTAGG
BalI 1908 TGGCCA TGG^CCA
BamHI 174 GGATCC G^GATCC
BbeI 3652 GGCGCC GGCGC^C
BbrPI 2443 CACGTG CAC^GTG
BbvCI 1093 CCTCAGC CCTCAGC(-5/-2)
BlnI 2275 CCTAGG C^CTAGG
BpmI 78 CTGGAG CTGGAG(16/14)
BsaAI 2443 CACGTG [CT]AC^GT[GA]
BsaHI 3652 GGCGCC G[GA]^CG[CT]C
BseAI 1492 TCCGGA T^CCGGA
BsiEI 208 CGGCCG CG[GA][CT]^CG
BsmI 438 GAATGC GAATGC(1/-1)
BsoBI 55 CCCGGG C^[CT]CG[GA]G
BspEI 1492 TCCGGA T^CCGGA
BsrFI 2229 ACCGGT [GA]^CCGG[CT]
BstAPI 3108 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 772 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2229 ACCGGT [GA]^CCGG[CT]
DraII 2271 GGGACCT [GA]G^G[ACGT]CC[CT]
EagI 208 CGGCCG C^GGCCG
Eam1105I 2228 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 208 CGGCCG C^GGCCG
Eco52I 208 CGGCCG C^GGCCG
Eco81I 772 CCTGAGG CC^T[ACGT]AGG
EcoNI 714 CCTATTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2271 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 674 GAATTC G^AATTC
EcoRV 2459 GATATC GAT^ATC
EcoT22I 3377 ATGCAT ATGCA^T
HgaI 785 GACGC GACGC(5/10)
Hin1I 3652 GGCGCC G[GA]^CG[CT]C
KasI 3652 GGCGCC G^GCGCC
KspI 46 CCGCGG CCGC^GG
MluI 816 ACGCGT A^CGCGT
MluNI 1908 TGGCCA TGG^CCA
MroI 1492 TCCGGA T^CCGGA
MscI 1908 TGGCCA TGG^CCA
NarI 3652 GGCGCC GG^CGCC
NcoI 2336 CCATGG C^CATGG
NotI 207 GCGGCCGC GC^GGCCGC
NsiI 3377 ATGCAT ATGCA^T
PacI 923 TTAATTAA TTAAT^TAA
PinAI 2229 ACCGGT A^CCGGT
PmaCI 2443 CACGTG CAC^GTG
PmlI 2443 CACGTG CAC^GTG
PpuMI 2271 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 3366 AACGTT AA^CGTT
SacII 46 CCGCGG CCGC^GG
SapI 3594 GAAGAGC GCTCTTC(1/4)
SbfI 883 CCTGCAGG CCTGCA^GG
SfiI 111 GGCCGTGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3652 GGCGCC GGC^GCC
SmaI 55 CCCGGG CCC^GGG
Sse8387I 883 CCTGCAGG CCTGCA^GG
TaqI 2845 TCGA T^CGA
TthHB8I 2845 TCGA T^CGA
XbaI 3221 TCTAGA T^CTAGA
XmaCI 55 CCCGGG C^CCGGG
XmaI 55 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 39 GGTCC G^G[AT]CC
2272 GGACC
BanII 60 GAGCCC G[GA]GC[CT]^C
1789 GAGCCC
BglI 112 GCCGTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
126 GCCCAGGAGGC
BsaI 1259 GGTCTC GGTCTC(1/5)
2235 GGTCTC
Bsp1407I 766 TGTACA T^GTACA
2697 TGTACA
BspHI 306 TCATGA T^CATGA
732 TCATGA
BspLU11I 704 ACATGT A^CATGT
763 ACATGT
BspMI 3389 ACCTGC ACCTGC(4/8)
886 GCAGGT
BsrGI 766 TGTACA T^GTACA
2697 TGTACA
BstXI 3011 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3238 CCAGGCAGCTGG
DsaI 46 CCGCGG C^C[GA][CT]GG
2336 CCATGG
EaeI 208 CGGCCG [CT]^GGCC[GA]
1908 TGGCCA
EcoT14I 2275 CCTAGG C^C[AT][AT]GG
2336 CCATGG
HaeII 184 AGCGCC [GA]GCGC^[CT]
3652 GGCGCC
PflMI 1146 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2876 CCAAGTGCTGG
RcaI 306 TCATGA T^CATGA
732 TCATGA
SmlI 561 CTCAAG C^T[CT][GA]AG
2430 CTTGAG
SspBI 766 TGTACA T^GTACA
2697 TGTACA
SspI 918 AATATT AAT^ATT
2548 AATATT
StyI 2275 CCTAGG C^C[AT][AT]GG
2336 CCATGG
Van91I 1146 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2876 CCAAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI BanI BbsI BbvI BcnI
BfaI BglII BmyI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII