##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI BsmBI
BsrBI BssHII BstAPI BstBI CelII
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoT22I
FseI FspI KasI KpnI KspI
MamI NarI NcoI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI PvuI
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 3451 GTATAC GT^[AC][GT]AC
AccIII 2479 TCCGGA T^CCGGA
AclI 1500 AACGTT AA^CGTT
AocI 3117 CCTGAGG CC^T[ACGT]AGG
AvaI 120 CCCGAG C^[CT]CG[GA]G
BamHI 1963 GGATCC G^GATCC
BanI 2490 GGTGCC G^G[CT][GA]CC
BbvCI 207 GCTGAGG CCTCAGC(-5/-2)
BglII 3527 AGATCT A^GATCT
BsaWI 2479 TCCGGA [AT]^CCGG[AT]
BseAI 2479 TCCGGA T^CCGGA
BsiEI 2073 CGGTCG CG[GA][CT]^CG
BsmI 1712 GAATGC GAATGC(1/-1)
BsoBI 120 CCCGAG C^[CT]CG[GA]G
BspDI 1317 ATCGAT AT^CGAT
BspEI 2479 TCCGGA T^CCGGA
BspLU11I 3409 ACATGT A^CATGT
Bst1107I 3451 GTATAC GTA^TAC
BstEII 2006 GGTTACC G^GT[ACGT]ACC
BstPI 2006 GGTTACC G^GT[ACGT]ACC
BstZ17I 3451 GTATAC GTA^TAC
Bsu36I 3117 CCTGAGG CC^T[ACGT]AGG
ClaI 1317 ATCGAT AT^CGAT
CpoI 2850 CGGACCG CG^G[AT]CCG
DrdI 954 GACAAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3117 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2006 GGTTACC G^GT[ACGT]ACC
EcoRV 2244 GATATC GAT^ATC
HaeII 1913 AGCGCC [GA]GCGC^[CT]
HincII 2997 GTTAAC GT[CT]^[GA]AC
HindII 2997 GTTAAC GT[CT]^[GA]AC
HindIII 9 AAGCTT A^AGCTT
HpaI 2997 GTTAAC GTT^AAC
MfeI 1453 CAATTG C^AATTG
MluI 1064 ACGCGT A^CGCGT
MroI 2479 TCCGGA T^CCGGA
MunI 1453 CAATTG C^AATTG
NaeI 2312 GCCGGC GCC^GGC
NdeI 387 CATATG CA^TATG
NgoMIV 2312 GCCGGC G^CCGGC
NheI 2595 GCTAGC G^CTAGC
PpuMI 1731 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2565 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1500 AACGTT AA^CGTT
RsrII 2850 CGGACCG CG^G[AT]CCG
ScaI 1407 AGTACT AGT^ACT
SmlI 2805 CTTGAG C^T[CT][GA]AG
SphI 2308 GCATGC GCATG^C
StuI 3077 AGGCCT AGG^CCT
XbaI 2046 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AatII 27 GACGTC GACGT^C
2565 GACGTC
AccII 1065 CGCG CG^CG
2071 CGCG
AcyI 27 GACGTC G[GA]^CG[CT]C
2565 GACGTC
Alw44I 510 GTGCAC G^TGCAC
677 GTGCAC
ApaLI 510 GTGCAC G^TGCAC
677 GTGCAC
Asp700I 1538 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2765 GAAGCTGTTC
AspI 24 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1238 GACAGAGTC
BbsI 1542 GTCTTC GAAGAC(2/6)
3112 GTCTTC
BciVI 1635 GGATAC GTATCC(6/5)
398 GTATCC
BclI 781 TGATCA T^GATCA
1783 TGATCA
BpuAI 1542 GTCTTC GAAGAC(2/6)
3112 GTCTTC
BsaHI 27 GACGTC G[GA]^CG[CT]C
2565 GACGTC
Bsp1407I 2704 TGTACA T^GTACA
3345 TGTACA
BspHI 319 TCATGA T^CATGA
1465 TCATGA
BspMI 166 ACCTGC ACCTGC(4/8)
3092 GCAGGT
BsrDI 567 CATTGC GCAATG(2/0)
1091 CATTGC
BsrGI 2704 TGTACA T^GTACA
3345 TGTACA
BstUI 1065 CGCG CG^CG
2071 CGCG
DraII 1731 GGGACCT [GA]G^G[ACGT]CC[CT]
2791 AGGGCCT
EarI 106 CTCTTC CTCTTC(1/4)
2869 GAAGAG
EcoO109I 1731 GGGACCT [GA]G^G[ACGT]CC[CT]
2791 AGGGCCT
EcoRI 1151 GAATTC G^AATTC
1472 GAATTC
FbaI 781 TGATCA T^GATCA
1783 TGATCA
HgaI 1334 GACGC GACGC(5/10)
459 GCGTC
Hin1I 27 GACGTC G[GA]^CG[CT]C
2565 GACGTC
Ksp632I 106 CTCTTC CTCTTC(1/4)
2869 GAAGAG
MvnI 1065 CGCG CG^CG
2071 CGCG
NspI 2308 GCATGC [GA]CATG^[CT]
3409 ACATGT
PflFI 24 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1238 GACAGAGTC
PvuII 793 CAGCTG CAG^CTG
2649 CAGCTG
RcaI 319 TCATGA T^CATGA
1465 TCATGA
SspBI 2704 TGTACA T^GTACA
3345 TGTACA
SspI 603 AATATT AAT^ATT
2584 AATATT
Tth111I 24 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1238 GACAGAGTC
XcmI 727 CCACAGACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1610 CCATGACATTGATGG
XmnI 1538 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2765 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII