##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AhdI AlwNI AocI
Aor51HI AscI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BciVI BfrI BglI BlnI
BsaAI BsaHI BseAI BsiWI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1239 GTCTAC GT^[AC][GT]AC
AgeI 1927 ACCGGT A^CCGGT
Alw44I 258 GTGCAC G^TGCAC
ApaLI 258 GTGCAC G^TGCAC
AspI 2005 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2406 CCTCAGC CCTCAGC(-5/-2)
BcgI 1745 CGACCTCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 228 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 228 GCTGAGC GC^T[ACGT]AGC
BsaBI 779 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2015 GGTCTC GGTCTC(1/5)
BsiEI 1899 CGACCG CG[GA][CT]^CG
BsmBI 403 CGTCTC CGTCTC(1/5)
BsmFI 2023 GTCCC GGGAC(10/14)
BsrBI 1987 CCGCTC CCGCTC(-3/-3)
BsrFI 1927 ACCGGT [GA]^CCGG[CT]
BstAPI 1597 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1360 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 228 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1927 ACCGGT [GA]^CCGG[CT]
EcoNI 1790 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1904 GAATTC G^AATTC
HaeII 765 AGCGCC [GA]GCGC^[CT]
MaeII 172 ACGT A^CGT
MamI 779 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1093 CAATTG C^AATTG
MunI 1093 CAATTG C^AATTG
PaeR7I 533 CTCGAG C^TCGAG
PflFI 2005 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2415 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1927 ACCGGT A^CCGGT
SapI 354 GCTCTTC GCTCTTC(1/4)
ScaI 1165 AGTACT AGT^ACT
SexAI 1510 ACCTGGT A^CC[AT]GGT
SphI 1058 GCATGC GCATG^C
Tth111I 2005 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2415 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1850 TCTAGA T^CTAGA
XcmI 2615 CCAGCCCAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 533 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 22 ACATGT A^C[GA][CT]GT
160 ACATGT
ApaI 134 GGGCCC GGGCC^C
1554 GGGCCC
AspHI 258 GTGCAC G[AT]GC[AT]^C
1781 GTGCTC
AvaI 131 CTCGGG C^[CT]CG[GA]G
533 CTCGAG
BanI 587 GGTGCC G^G[CT][GA]CC
2310 GGCACC
BclI 1696 TGATCA T^GATCA
1717 TGATCA
BcnI 670 CCCGG CC^[GC]GG
2696 CCCGG
BglII 676 AGATCT A^GATCT
1329 AGATCT
BsaWI 1927 ACCGGT [AT]^CCGG[AT]
2019 TCCGGT
BseRI 430 CTCCTC GAGGAG(10/8)
1161 GAGGAG
BsiHKAI 258 GTGCAC G[AT]GC[AT]^C
1781 GTGCTC
BsmI 320 GAATGC GAATGC(1/-1)
1619 GAATGC
BsoBI 131 CTCGGG C^[CT]CG[GA]G
533 CTCGAG
BspLU11I 22 ACATGT A^CATGT
160 ACATGT
BssSI 2113 CTCGTG CACGAG(-5/-1)
2283 CTCGTG
CfoI 766 GCGC GCG^C
2499 GCGC
FbaI 1696 TGATCA T^GATCA
1717 TGATCA
HhaI 766 GCGC GCG^C
2499 GCGC
HinP1I 766 GCGC G^CGC
2499 GCGC
MspA1I 1818 CAGCTG C[AC]G^C[GT]G
1936 CAGCTG
NciI 670 CCCGG CC^[GC]GG
2696 CCCGG
PvuII 1818 CAGCTG CAG^CTG
1936 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiYI BslI
BsmAI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII