################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AhdI AlwNI AocI Aor51HI AscI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BciVI BfrI BglI BlnI BsaAI BsaHI BseAI BsiWI Bsp1407I BspDI BspEI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 1239 GTCTAC GT^[AC][GT]AC AgeI 1927 ACCGGT A^CCGGT Alw44I 258 GTGCAC G^TGCAC ApaLI 258 GTGCAC G^TGCAC AspI 2005 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 2406 CCTCAGC CCTCAGC(-5/-2) BcgI 1745 CGACCTCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 228 GCTGAGC GC^T[ACGT]AGC Bpu1102I 228 GCTGAGC GC^T[ACGT]AGC BsaBI 779 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2015 GGTCTC GGTCTC(1/5) BsiEI 1899 CGACCG CG[GA][CT]^CG BsmBI 403 CGTCTC CGTCTC(1/5) BsmFI 2023 GTCCC GGGAC(10/14) BsrBI 1987 CCGCTC CCGCTC(-3/-3) BsrFI 1927 ACCGGT [GA]^CCGG[CT] BstAPI 1597 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1360 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 228 GCTGAGC GC^T[ACGT]AGC Cfr10I 1927 ACCGGT [GA]^CCGG[CT] EcoNI 1790 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1904 GAATTC G^AATTC HaeII 765 AGCGCC [GA]GCGC^[CT] MaeII 172 ACGT A^CGT MamI 779 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1093 CAATTG C^AATTG MunI 1093 CAATTG C^AATTG PaeR7I 533 CTCGAG C^TCGAG PflFI 2005 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2415 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1927 ACCGGT A^CCGGT SapI 354 GCTCTTC GCTCTTC(1/4) ScaI 1165 AGTACT AGT^ACT SexAI 1510 ACCTGGT A^CC[AT]GGT SphI 1058 GCATGC GCATG^C Tth111I 2005 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2415 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1850 TCTAGA T^CTAGA XcmI 2615 CCAGCCCAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 533 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 22 ACATGT A^C[GA][CT]GT 160 ACATGT ApaI 134 GGGCCC GGGCC^C 1554 GGGCCC AspHI 258 GTGCAC G[AT]GC[AT]^C 1781 GTGCTC AvaI 131 CTCGGG C^[CT]CG[GA]G 533 CTCGAG BanI 587 GGTGCC G^G[CT][GA]CC 2310 GGCACC BclI 1696 TGATCA T^GATCA 1717 TGATCA BcnI 670 CCCGG CC^[GC]GG 2696 CCCGG BglII 676 AGATCT A^GATCT 1329 AGATCT BsaWI 1927 ACCGGT [AT]^CCGG[AT] 2019 TCCGGT BseRI 430 CTCCTC GAGGAG(10/8) 1161 GAGGAG BsiHKAI 258 GTGCAC G[AT]GC[AT]^C 1781 GTGCTC BsmI 320 GAATGC GAATGC(1/-1) 1619 GAATGC BsoBI 131 CTCGGG C^[CT]CG[GA]G 533 CTCGAG BspLU11I 22 ACATGT A^CATGT 160 ACATGT BssSI 2113 CTCGTG CACGAG(-5/-1) 2283 CTCGTG CfoI 766 GCGC GCG^C 2499 GCGC FbaI 1696 TGATCA T^GATCA 1717 TGATCA HhaI 766 GCGC GCG^C 2499 GCGC HinP1I 766 GCGC G^CGC 2499 GCGC MspA1I 1818 CAGCTG C[AC]G^C[GT]G 1936 CAGCTG NciI 670 CCCGG CC^[GC]GG 2696 CCCGG PvuII 1818 CAGCTG CAG^CTG 1936 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII