##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaLI       
AscI        Asp718I     AspEI       AspHI       AvaI        
AviII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BcnI        BsaBI       BsaHI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstXI       Cac8I       CfoI        Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
FauI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HpaI        KasI        KpnI        
KspI        MamI        MluI        MroI        MspA1I      
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SpeI        SphI        Sse8387I    StuI        SwaI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII          53  CGCG                 CG^CG
AciI           54  GCGG                 CCGC(-3/-1)
AclI         3708  AACGTT               AA^CGTT
AlwNI        3398  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2748  CCTCAGG              CC^T[ACGT]AGG
ApaI          352  GGGCCC               GGGCC^C
AspI          613  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3089  CCTAGG               C^CTAGG
BalI         3178  TGGCCA               TGG^CCA
BanII         352  GGGCCC               G[GA]GC[CT]^C
BglI         2747  GCCTCAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3249  AGATCT               A^GATCT
BlnI         3089  CCTAGG               C^CTAGG
BlpI         3069  GCTTAGC              GC^T[ACGT]AGC
BmyI          352  GGGCCC               G[AGT]GC[ACT]^C
Bpu1102I     3069  GCTTAGC              GC^T[ACGT]AGC
BsaAI         554  TACGTA               [CT]AC^GT[GA]
BsaI         3208  GGTCTC               GGTCTC(1/5)
BsaWI        1911  ACCGGA               [AT]^CCGG[AT]
Bsp1286I      352  GGGCCC               G[AGT]GC[ACT]^C
Bsp1407I     2249  TGTACA               T^GTACA
BsrGI        2249  TGTACA               T^GTACA
Bst1107I     1217  GTATAC               GTA^TAC
BstAPI       2281  GCATTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          53  CGCG                 CG^CG
BstYI        3249  AGATCT               [GA]^GATC[CT]
BstZ17I      1217  GTATAC               GTA^TAC
Bsu36I       2748  CCTCAGG              CC^T[ACGT]AGG
CelII        3069  GCTTAGC              GC^T[ACGT]AGC
DraIII         43  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1324  CCGTGG               C^C[GA][CT]GG
EaeI         3178  TGGCCA               [CT]^GGCC[GA]
Eco81I       2748  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2744  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI           51  GACGC                GACGC(5/10)
HincII       2192  GTTGAC               GT[CT]^[GA]AC
HindII       2192  GTTGAC               GT[CT]^[GA]AC
MfeI         2453  CAATTG               C^AATTG
MflI         3249  AGATCT               [GA]^GATC[CT]
MluNI        3178  TGGCCA               TGG^CCA
MscI         3178  TGGCCA               TGG^CCA
MunI         2453  CAATTG               C^AATTG
MvnI           53  CGCG                 CG^CG
PflFI         613  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3103  CCAAGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         176  GACAGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3708  AACGTT               AA^CGTT
PstI         3483  CTGCAG               CTGCA^G
SexAI        3047  ACCTGGT              A^CC[AT]GGT
SfcI         3483  CTGCAG               C^T[GA][CT]AG
SnaBI         554  TACGTA               TAC^GTA
SspBI        2249  TGTACA               T^GTACA
Tth111I       613  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3103  CCAAGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         3380  CCATCTGATCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        3249  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         1217  GTATAC               GT^[AC][GT]AC
             2344  GTAGAC              
AflII         809  CTTAAG               C^TTAAG
             3715  CTTAAG              
AflIII       2027  ACATGT               A^C[GA][CT]GT
             2377  ACATGT              
Asp700I       841  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1036  GAAAGTATTC          
AvaII           3  GGACC                G^G[AT]CC
             3093  GGACC               
BciVI        3393  GGATAC               GTATCC(6/5)
             3418  GGATAC              
BclI         2971  TGATCA               T^GATCA
             3385  TGATCA              
BfrI          809  CTTAAG               C^TTAAG
             3715  CTTAAG              
BsgI         3423  CTGCAC               GTGCAG(16/14)
              425  GTGCAG              
BspHI         528  TCATGA               T^CATGA
             2210  TCATGA              
BspLU11I     2027  ACATGT               A^CATGT
             2377  ACATGT              
BspMI         287  ACCTGC               ACCTGC(4/8)
             2743  ACCTGC              
DraI         2322  TTTAAA               TTT^AAA
             3554  TTTAAA              
EarI         1376  CTCTTC               CTCTTC(1/4)
             3213  CTCTTC              
EcoT14I      3089  CCTAGG               C^C[AT][AT]GG
             3103  CCAAGG              
EcoT22I      2244  ATGCAT               ATGCA^T
             3648  ATGCAT              
FbaI         2971  TGATCA               T^GATCA
             3385  TGATCA              
HapII           1  CCGG                 C^CGG
             1912  CCGG                
HpaII           1  CCGG                 C^CGG
             1912  CCGG                
Ksp632I      1376  CTCTTC               CTCTTC(1/4)
             3213  CTCTTC              
MspI            1  CCGG                 C^CGG
             1912  CCGG                
NdeI         2105  CATATG               CA^TATG
             2296  CATATG              
NsiI         2244  ATGCAT               ATGCA^T
             3648  ATGCAT              
RcaI          528  TCATGA               T^CATGA
             2210  TCATGA              
SspI          433  AATATT               AAT^ATT
             2655  AATATT              
StyI         3089  CCTAGG               C^C[AT][AT]GG
             3103  CCAAGG              
XmnI          841  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1036  GAAAGTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AseI        
AsnI        BbsI        BbvI        BfaI        BpmI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsrDI       BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRI       EcoRII      Fnu4HI      
FokI        HaeIII      HindIII     HinfI       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PshBI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I