##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
AscI Asp718I AspEI AspHI AvaI
AviII BamHI BanI BbeI BbrPI
BbvCI BcgI BcnI BsaBI BsaHI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstXI Cac8I CfoI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MluI MroI MspA1I
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I StuI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 53 CGCG CG^CG
AciI 54 GCGG CCGC(-3/-1)
AclI 3708 AACGTT AA^CGTT
AlwNI 3398 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2748 CCTCAGG CC^T[ACGT]AGG
ApaI 352 GGGCCC GGGCC^C
AspI 613 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3089 CCTAGG C^CTAGG
BalI 3178 TGGCCA TGG^CCA
BanII 352 GGGCCC G[GA]GC[CT]^C
BglI 2747 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3249 AGATCT A^GATCT
BlnI 3089 CCTAGG C^CTAGG
BlpI 3069 GCTTAGC GC^T[ACGT]AGC
BmyI 352 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 3069 GCTTAGC GC^T[ACGT]AGC
BsaAI 554 TACGTA [CT]AC^GT[GA]
BsaI 3208 GGTCTC GGTCTC(1/5)
BsaWI 1911 ACCGGA [AT]^CCGG[AT]
Bsp1286I 352 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 2249 TGTACA T^GTACA
BsrGI 2249 TGTACA T^GTACA
Bst1107I 1217 GTATAC GTA^TAC
BstAPI 2281 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 53 CGCG CG^CG
BstYI 3249 AGATCT [GA]^GATC[CT]
BstZ17I 1217 GTATAC GTA^TAC
Bsu36I 2748 CCTCAGG CC^T[ACGT]AGG
CelII 3069 GCTTAGC GC^T[ACGT]AGC
DraIII 43 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1324 CCGTGG C^C[GA][CT]GG
EaeI 3178 TGGCCA [CT]^GGCC[GA]
Eco81I 2748 CCTCAGG CC^T[ACGT]AGG
EcoNI 2744 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 51 GACGC GACGC(5/10)
HincII 2192 GTTGAC GT[CT]^[GA]AC
HindII 2192 GTTGAC GT[CT]^[GA]AC
MfeI 2453 CAATTG C^AATTG
MflI 3249 AGATCT [GA]^GATC[CT]
MluNI 3178 TGGCCA TGG^CCA
MscI 3178 TGGCCA TGG^CCA
MunI 2453 CAATTG C^AATTG
MvnI 53 CGCG CG^CG
PflFI 613 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3103 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 176 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3708 AACGTT AA^CGTT
PstI 3483 CTGCAG CTGCA^G
SexAI 3047 ACCTGGT A^CC[AT]GGT
SfcI 3483 CTGCAG C^T[GA][CT]AG
SnaBI 554 TACGTA TAC^GTA
SspBI 2249 TGTACA T^GTACA
Tth111I 613 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3103 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3380 CCATCTGATCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3249 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1217 GTATAC GT^[AC][GT]AC
2344 GTAGAC
AflII 809 CTTAAG C^TTAAG
3715 CTTAAG
AflIII 2027 ACATGT A^C[GA][CT]GT
2377 ACATGT
Asp700I 841 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1036 GAAAGTATTC
AvaII 3 GGACC G^G[AT]CC
3093 GGACC
BciVI 3393 GGATAC GTATCC(6/5)
3418 GGATAC
BclI 2971 TGATCA T^GATCA
3385 TGATCA
BfrI 809 CTTAAG C^TTAAG
3715 CTTAAG
BsgI 3423 CTGCAC GTGCAG(16/14)
425 GTGCAG
BspHI 528 TCATGA T^CATGA
2210 TCATGA
BspLU11I 2027 ACATGT A^CATGT
2377 ACATGT
BspMI 287 ACCTGC ACCTGC(4/8)
2743 ACCTGC
DraI 2322 TTTAAA TTT^AAA
3554 TTTAAA
EarI 1376 CTCTTC CTCTTC(1/4)
3213 CTCTTC
EcoT14I 3089 CCTAGG C^C[AT][AT]GG
3103 CCAAGG
EcoT22I 2244 ATGCAT ATGCA^T
3648 ATGCAT
FbaI 2971 TGATCA T^GATCA
3385 TGATCA
HapII 1 CCGG C^CGG
1912 CCGG
HpaII 1 CCGG C^CGG
1912 CCGG
Ksp632I 1376 CTCTTC CTCTTC(1/4)
3213 CTCTTC
MspI 1 CCGG C^CGG
1912 CCGG
NdeI 2105 CATATG CA^TATG
2296 CATATG
NsiI 2244 ATGCAT ATGCA^T
3648 ATGCAT
RcaI 528 TCATGA T^CATGA
2210 TCATGA
SspI 433 AATATT AAT^ATT
2655 AATATT
StyI 3089 CCTAGG C^C[AT][AT]GG
3103 CCAAGG
XmnI 841 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1036 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BbsI BbvI BfaI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsmI BsrDI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraII EcoO109I EcoRI EcoRII Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I