################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI Asp718I AspEI AspHI AvaI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BsaBI BsaHI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstXI Cac8I CfoI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FauI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MluI MroI MspA1I NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 53 CGCG CG^CG AciI 54 GCGG CCGC(-3/-1) AclI 3708 AACGTT AA^CGTT AlwNI 3398 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2748 CCTCAGG CC^T[ACGT]AGG ApaI 352 GGGCCC GGGCC^C AspI 613 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3089 CCTAGG C^CTAGG BalI 3178 TGGCCA TGG^CCA BanII 352 GGGCCC G[GA]GC[CT]^C BglI 2747 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3249 AGATCT A^GATCT BlnI 3089 CCTAGG C^CTAGG BlpI 3069 GCTTAGC GC^T[ACGT]AGC BmyI 352 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 3069 GCTTAGC GC^T[ACGT]AGC BsaAI 554 TACGTA [CT]AC^GT[GA] BsaI 3208 GGTCTC GGTCTC(1/5) BsaWI 1911 ACCGGA [AT]^CCGG[AT] Bsp1286I 352 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 2249 TGTACA T^GTACA BsrGI 2249 TGTACA T^GTACA Bst1107I 1217 GTATAC GTA^TAC BstAPI 2281 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 53 CGCG CG^CG BstYI 3249 AGATCT [GA]^GATC[CT] BstZ17I 1217 GTATAC GTA^TAC Bsu36I 2748 CCTCAGG CC^T[ACGT]AGG CelII 3069 GCTTAGC GC^T[ACGT]AGC DraIII 43 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1324 CCGTGG C^C[GA][CT]GG EaeI 3178 TGGCCA [CT]^GGCC[GA] Eco81I 2748 CCTCAGG CC^T[ACGT]AGG EcoNI 2744 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 51 GACGC GACGC(5/10) HincII 2192 GTTGAC GT[CT]^[GA]AC HindII 2192 GTTGAC GT[CT]^[GA]AC MfeI 2453 CAATTG C^AATTG MflI 3249 AGATCT [GA]^GATC[CT] MluNI 3178 TGGCCA TGG^CCA MscI 3178 TGGCCA TGG^CCA MunI 2453 CAATTG C^AATTG MvnI 53 CGCG CG^CG PflFI 613 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3103 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 176 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3708 AACGTT AA^CGTT PstI 3483 CTGCAG CTGCA^G SexAI 3047 ACCTGGT A^CC[AT]GGT SfcI 3483 CTGCAG C^T[GA][CT]AG SnaBI 554 TACGTA TAC^GTA SspBI 2249 TGTACA T^GTACA Tth111I 613 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3103 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3380 CCATCTGATCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 3249 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1217 GTATAC GT^[AC][GT]AC 2344 GTAGAC AflII 809 CTTAAG C^TTAAG 3715 CTTAAG AflIII 2027 ACATGT A^C[GA][CT]GT 2377 ACATGT Asp700I 841 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1036 GAAAGTATTC AvaII 3 GGACC G^G[AT]CC 3093 GGACC BciVI 3393 GGATAC GTATCC(6/5) 3418 GGATAC BclI 2971 TGATCA T^GATCA 3385 TGATCA BfrI 809 CTTAAG C^TTAAG 3715 CTTAAG BsgI 3423 CTGCAC GTGCAG(16/14) 425 GTGCAG BspHI 528 TCATGA T^CATGA 2210 TCATGA BspLU11I 2027 ACATGT A^CATGT 2377 ACATGT BspMI 287 ACCTGC ACCTGC(4/8) 2743 ACCTGC DraI 2322 TTTAAA TTT^AAA 3554 TTTAAA EarI 1376 CTCTTC CTCTTC(1/4) 3213 CTCTTC EcoT14I 3089 CCTAGG C^C[AT][AT]GG 3103 CCAAGG EcoT22I 2244 ATGCAT ATGCA^T 3648 ATGCAT FbaI 2971 TGATCA T^GATCA 3385 TGATCA HapII 1 CCGG C^CGG 1912 CCGG HpaII 1 CCGG C^CGG 1912 CCGG Ksp632I 1376 CTCTTC CTCTTC(1/4) 3213 CTCTTC MspI 1 CCGG C^CGG 1912 CCGG NdeI 2105 CATATG CA^TATG 2296 CATATG NsiI 2244 ATGCAT ATGCA^T 3648 ATGCAT RcaI 528 TCATGA T^CATGA 2210 TCATGA SspI 433 AATATT AAT^ATT 2655 AATATT StyI 3089 CCTAGG C^C[AT][AT]GG 3103 CCAAGG XmnI 841 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1036 GAAAGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI BbsI BbvI BfaI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI BsrDI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRI EcoRII Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I