##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspI AviII BbeI BbrPI BcgI
BciVI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoNI EcoO65I
FseI FspI HindIII HpaI KasI
KpnI KspI MfeI MroI MunI
NarI NdeI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 2202 CTTAAG C^TTAAG
AflIII 931 ACGCGT A^C[GA][CT]GT
BamHI 2103 GGATCC G^GATCC
BbsI 344 GTCTTC GAAGAC(2/6)
BbvCI 790 CCTCAGC CCTCAGC(-5/-2)
BclI 1110 TGATCA T^GATCA
BfrI 2202 CTTAAG C^TTAAG
BglII 1484 AGATCT A^GATCT
BlpI 1851 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1851 GCTCAGC GC^T[ACGT]AGC
BpuAI 344 GTCTTC GAAGAC(2/6)
BsaAI 512 TACGTG [CT]AC^GT[GA]
BsaBI 1105 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1872 CGACCG CG[GA][CT]^CG
BsmI 1255 GAATGC GAATGC(1/-1)
BsrBI 977 GAGCGG CCGCTC(-3/-3)
BssHII 305 GCGCGC G^CGCGC
BstXI 1391 CCAAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1851 GCTCAGC GC^T[ACGT]AGC
DrdI 173 GACTGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1691 GAATTC G^AATTC
EcoRV 659 GATATC GAT^ATC
EcoT22I 684 ATGCAT ATGCA^T
FauI 666 CCCGC CCCGC(4/6)
FbaI 1110 TGATCA T^GATCA
MamI 1105 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 931 ACGCGT A^CGCGT
NheI 2831 GCTAGC G^CTAGC
NsiI 684 ATGCAT ATGCA^T
PflMI 2215 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3110 AGGTCCC [GA]G^G[AT]CC[CT]
SbfI 778 CCTGCAGG CCTGCA^GG
SexAI 1089 ACCTGGT A^CC[AT]GGT
SphI 249 GCATGC GCATG^C
Sse8387I 778 CCTGCAGG CCTGCA^GG
SspI 1046 AATATT AAT^ATT
StuI 962 AGGCCT AGG^CCT
Van91I 2215 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3037 CCAGTGCCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1663 GACGTC GACGT^C
2128 GACGTC
AhdI 2110 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2757 GACTTCCTGTC
AocI 2167 CCTTAGG CC^T[ACGT]AGG
2317 CCTTAGG
Aor51HI 2393 AGCGCT AGC^GCT
2660 AGCGCT
Asp700I 1959 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3091 GAATTGATTC
AspEI 2110 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2757 GACTTCCTGTC
AspHI 611 GAGCTC G[AT]GC[AT]^C
2303 GAGCTC
AvrII 1232 CCTAGG C^CTAGG
3023 CCTAGG
BalI 1188 TGGCCA TGG^CCA
2540 TGGCCA
BanI 1840 GGCACC G^G[CT][GA]CC
3075 GGCACC
BglI 492 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1731 GCCCAGCCGGC
BlnI 1232 CCTAGG C^CTAGG
3023 CCTAGG
BsaI 8 GAGACC GGTCTC(1/5)
1119 GAGACC
BseRI 2285 CTCCTC GAGGAG(10/8)
290 GAGGAG
BsgI 486 GTGCAG GTGCAG(16/14)
1208 GTGCAG
BsiHKAI 611 GAGCTC G[AT]GC[AT]^C
2303 GAGCTC
BsrDI 2016 CATTGC GCAATG(2/0)
365 GCAATG
Bsu36I 2167 CCTTAGG CC^T[ACGT]AGG
2317 CCTTAGG
DraIII 731 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1879 CACTTTGTG
Eam1105I 2110 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2757 GACTTCCTGTC
Eco47III 2393 AGCGCT AGC^GCT
2660 AGCGCT
Eco81I 2167 CCTTAGG CC^T[ACGT]AGG
2317 CCTTAGG
HaeII 2393 AGCGCT [GA]GCGC^[CT]
2660 AGCGCT
MluNI 1188 TGGCCA TGG^CCA
2540 TGGCCA
MscI 1188 TGGCCA TGG^CCA
2540 TGGCCA
NaeI 1736 GCCGGC GCC^GGC
2045 GCCGGC
NgoMIV 1736 GCCGGC G^CCGGC
2045 GCCGGC
PaeR7I 1289 CTCGAG C^TCGAG
2465 CTCGAG
SacI 611 GAGCTC GAGCT^C
2303 GAGCTC
SalI 27 GTCGAC G^TCGAC
2432 GTCGAC
SmaI 493 CCCGGG CCC^GGG
1550 CCCGGG
XhoI 1289 CTCGAG C^TCGAG
2465 CTCGAG
XmaCI 493 CCCGGG C^CCGGG
1550 CCCGGG
XmaI 493 CCCGGG C^CCGGG
1550 CCCGGG
XmnI 1959 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3091 GAATTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII