##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbeI BcgI BclI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
Bst1107I BstBI BstXI BstZ17I ClaI
DraI DraIII DrdI Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KasI KpnI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 2603 GAATTT [GA]^AATT[CT]
AgeI 1371 ACCGGT A^CCGGT
AhdI 775 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 762 GGGCCC GGGCC^C
ApoI 2603 GAATTT [GA]^AATT[CT]
AspEI 775 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1938 CCTAGG C^CTAGG
BbrPI 1150 CACGTG CAC^GTG
BglI 1069 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1938 CCTAGG C^CTAGG
BsaAI 1150 CACGTG [CT]AC^GT[GA]
BsaBI 381 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 782 GGTCTC GGTCTC(1/5)
BsiEI 142 CGGCCG CG[GA][CT]^CG
BsmBI 250 GAGACG CGTCTC(1/5)
BspMI 224 ACCTGC ACCTGC(4/8)
BsrFI 1371 ACCGGT [GA]^CCGG[CT]
BssSI 290 CACGAG CACGAG(-5/-1)
BstEII 1246 GGTGACC G^GT[ACGT]ACC
BstPI 1246 GGTGACC G^GT[ACGT]ACC
Cfr10I 1371 ACCGGT [GA]^CCGG[CT]
CpoI 2382 CGGACCG CG^G[AT]CCG
EagI 142 CGGCCG C^GGCCG
Eam1105I 775 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1266 CTCTTC CTCTTC(1/4)
EclXI 142 CGGCCG C^GGCCG
Eco52I 142 CGGCCG C^GGCCG
EcoNI 1029 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1246 GGTGACC G^GT[ACGT]ACC
HindIII 1294 AAGCTT A^AGCTT
Ksp632I 1266 CTCTTC CTCTTC(1/4)
KspI 9 CCGCGG CCGC^GG
MamI 381 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1756 CAATTG C^AATTG
MunI 1756 CAATTG C^AATTG
PflMI 974 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1371 ACCGGT A^CCGGT
PmaCI 1150 CACGTG CAC^GTG
PmlI 1150 CACGTG CAC^GTG
RsrII 2382 CGGACCG CG^G[AT]CCG
SacI 297 GAGCTC GAGCT^C
SacII 9 CCGCGG CCGC^GG
TaqI 1446 TCGA T^CGA
TthHB8I 1446 TCGA T^CGA
Van91I 974 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 627 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 1771 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2459 GAAGCTGTTC
AspHI 297 GAGCTC G[AT]GC[AT]^C
2743 GAGCAC
BalI 314 TGGCCA TGG^CCA
2787 TGGCCA
BbvCI 1255 GCTGAGG CCTCAGC(-5/-2)
1961 GCTGAGG
BciVI 1260 GGATAC GTATCC(6/5)
1408 GTATCC
BglII 603 AGATCT A^GATCT
2437 AGATCT
BlpI 14 GCTCAGC GC^T[ACGT]AGC
1956 GCTCAGC
Bpu1102I 14 GCTCAGC GC^T[ACGT]AGC
1956 GCTCAGC
BsiHKAI 297 GAGCTC G[AT]GC[AT]^C
2743 GAGCAC
Bsp1407I 800 TGTACA T^GTACA
2670 TGTACA
BsrDI 417 CATTGC GCAATG(2/0)
2688 GCAATG
BsrGI 800 TGTACA T^GTACA
2670 TGTACA
BssHII 19 GCGCGC G^CGCGC
21 GCGCGC
BstAPI 354 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1146 GCAGCACGTGC
CelII 14 GCTCAGC GC^T[ACGT]AGC
1956 GCTCAGC
HaeII 92 AGCGCC [GA]GCGC^[CT]
136 AGCGCC
HincII 742 GTCAAC GT[CT]^[GA]AC
988 GTCAAC
HindII 742 GTCAAC GT[CT]^[GA]AC
988 GTCAAC
MluNI 314 TGGCCA TGG^CCA
2787 TGGCCA
MscI 314 TGGCCA TGG^CCA
2787 TGGCCA
NcoI 68 CCATGG C^CATGG
1073 CCATGG
PvuII 777 CAGCTG CAG^CTG
1959 CAGCTG
SspBI 800 TGTACA T^GTACA
2670 TGTACA
Tsp509I 1757 AATT ^AATT
2604 AATT
XcmI 68 CCATGGAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
699 CCAGACGTTCTGTGG
XmnI 1771 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2459 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AluI AlwI AlwNI AocI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I TspRI
XhoII