##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI BalI BbeI BbrPI
BcgI BciVI BclI BglII BsmBI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EcoRV
EcoT22I FbaI FseI HindIII KasI
KpnI KspI MfeI MluI MluNI
MscI MunI NarI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SexAI SfiI SfoI SgrAI
SnaBI SpeI SspBI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 2488 GTCTAC GT^[AC][GT]AC
AccII 153 CGCG CG^CG
AccIII 2378 TCCGGA T^CCGGA
AcyI 165 GGCGTC G[GA]^CG[CT]C
AflII 1129 CTTAAG C^TTAAG
Aor51HI 893 AGCGCT AGC^GCT
Asp700I 205 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 709 TGCGCA TGC^GCA
AvrII 544 CCTAGG C^CTAGG
BamHI 414 GGATCC G^GATCC
BbvCI 820 GCTGAGG CCTCAGC(-5/-2)
BfrI 1129 CTTAAG C^TTAAG
BlnI 544 CCTAGG C^CTAGG
BlpI 774 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 774 GCTGAGC GC^T[ACGT]AGC
BsaHI 165 GGCGTC G[GA]^CG[CT]C
BseAI 2378 TCCGGA T^CCGGA
BsiEI 149 CGGCCG CG[GA][CT]^CG
BsiWI 1350 CGTACG C^GTACG
BspEI 2378 TCCGGA T^CCGGA
BspLU11I 1225 ACATGT A^CATGT
BssSI 1337 CACGAG CACGAG(-5/-1)
BstAPI 2424 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2123 TTCGAA TT^CGAA
BstUI 153 CGCG CG^CG
BstXI 363 CCAGTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 774 GCTGAGC GC^T[ACGT]AGC
DsaI 311 CCACGG C^C[GA][CT]GG
EagI 149 CGGCCG C^GGCCG
EclXI 149 CGGCCG C^GGCCG
Eco47III 893 AGCGCT AGC^GCT
Eco52I 149 CGGCCG C^GGCCG
EcoNI 840 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2505 GAATTC G^AATTC
FspI 709 TGCGCA TGC^GCA
Hin1I 165 GGCGTC G[GA]^CG[CT]C
HpaI 2331 GTTAAC GTT^AAC
MroI 2378 TCCGGA T^CCGGA
MvnI 153 CGCG CG^CG
NspV 2123 TTCGAA TT^CGAA
PshAI 2129 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2421 CCTGCAGG CCTGCA^GG
ScaI 1133 AGTACT AGT^ACT
SfuI 2123 TTCGAA TT^CGAA
SmaI 854 CCCGGG CCC^GGG
SphI 2372 GCATGC GCATG^C
Sse8387I 2421 CCTGCAGG CCTGCA^GG
SspI 304 AATATT AAT^ATT
StuI 91 AGGCCT AGG^CCT
XmaCI 854 CCCGGG C^CCGGG
XmaI 854 CCCGGG C^CCGGG
XmnI 205 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 1843 GAATTT [GA]^AATT[CT]
2505 GAATTC
AflIII 1225 ACATGT A^C[GA][CT]GT
1269 ACGTGT
AgeI 133 ACCGGT A^CCGGT
1444 ACCGGT
ApoI 1843 GAATTT [GA]^AATT[CT]
2505 GAATTC
AspI 239 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1483 GACTGGGTC
AvaI 854 CCCGGG C^[CT]CG[GA]G
1477 CCCGAG
BanI 141 GGTGCC G^G[CT][GA]CC
743 GGTGCC
BglI 1255 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2269 GCCTCCCAGGC
BsaAI 1027 CACGTA [CT]AC^GT[GA]
1268 TACGTG
BsaBI 415 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1504 GATGCTGATC
BsaI 1441 GAGACC GGTCTC(1/5)
2459 GAGACC
BsmI 324 GAATGC GAATGC(1/-1)
2544 GCATTC
BsoBI 854 CCCGGG C^[CT]CG[GA]G
1477 CCCGAG
BstEII 849 GGTGACC G^GT[ACGT]ACC
2022 GGTGACC
BstPI 849 GGTGACC G^GT[ACGT]ACC
2022 GGTGACC
BstYI 414 GGATCC [GA]^GATC[CT]
1278 AGATCC
EaeI 149 CGGCCG [CT]^GGCC[GA]
1111 CGGCCA
EcoO65I 849 GGTGACC G^GT[ACGT]ACC
2022 GGTGACC
EcoT14I 369 CCTTGG C^C[AT][AT]GG
544 CCTAGG
HincII 1219 GTCAAC GT[CT]^[GA]AC
2331 GTTAAC
HindII 1219 GTCAAC GT[CT]^[GA]AC
2331 GTTAAC
MamI 415 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1504 GATGCTGATC
MflI 414 GGATCC [GA]^GATC[CT]
1278 AGATCC
NaeI 147 GCCGGC GCC^GGC
746 GCCGGC
NgoMIV 147 GCCGGC G^CCGGC
746 GCCGGC
PflFI 239 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1483 GACTGGGTC
PinAI 133 ACCGGT A^CCGGT
1444 ACCGGT
PvuII 772 CAGCTG CAG^CTG
2070 CAGCTG
SacI 103 GAGCTC GAGCT^C
482 GAGCTC
StyI 369 CCTTGG C^C[AT][AT]GG
544 CCTAGG
Tth111I 239 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1483 GACTGGGTC
XcmI 567 CCAGCGCCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2069 CCAGCTGGACCCTGG
XhoII 414 GGATCC [GA]^GATC[CT]
1278 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EarI Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I