##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BbrPI BcgI BciVI BclI
BglII BlnI BsaAI BsaBI BseAI
BsgI BsiWI BsmBI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 2465 CTTAAG C^TTAAG
ApaI 616 GGGCCC GGGCC^C
AseI 3101 ATTAAT AT^TAAT
AsnI 3101 ATTAAT AT^TAAT
BbsI 1476 GAAGAC GAAGAC(2/6)
BfrI 2465 CTTAAG C^TTAAG
BpuAI 1476 GAAGAC GAAGAC(2/6)
BsaI 378 GGTCTC GGTCTC(1/5)
BsaWI 669 ACCGGA [AT]^CCGG[AT]
BsmI 942 GAATGC GAATGC(1/-1)
BspLU11I 2645 ACATGT A^CATGT
BsrBI 1545 CCGCTC CCGCTC(-3/-3)
BsrDI 2880 CATTGC GCAATG(2/0)
BssSI 454 CTCGTG CACGAG(-5/-1)
DraI 2496 TTTAAA TTT^AAA
EcoNI 1713 CCTAAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3219 ATGCAT ATGCA^T
HincII 2259 GTTGAC GT[CT]^[GA]AC
HindII 2259 GTTGAC GT[CT]^[GA]AC
NcoI 621 CCATGG C^CATGG
NdeI 1968 CATATG CA^TATG
NheI 2355 GCTAGC G^CTAGC
NotI 432 GCGGCCGC GC^GGCCGC
NsiI 3219 ATGCAT ATGCA^T
NspI 2645 ACATGT [GA]CATG^[CT]
PshBI 3101 ATTAAT AT^TAAT
PstI 2318 CTGCAG CTGCA^G
PvuII 2233 CAGCTG CAG^CTG
ScaI 2930 AGTACT AGT^ACT
SfcI 2318 CTGCAG C^T[GA][CT]AG
SmaI 130 CCCGGG CCC^GGG
SmlI 2465 CTTAAG C^T[CT][GA]AG
SspI 2483 AATATT AAT^ATT
StuI 12 AGGCCT AGG^CCT
TfiI 2340 GAATC G^A[AT]TC
XbaI 2148 TCTAGA T^CTAGA
XmaCI 130 CCCGGG C^CCGGG
XmaI 130 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 1381 ACACGT A^C[GA][CT]GT
2645 ACATGT
AocI 265 CCTCAGG CC^T[ACGT]AGG
2514 CCTAAGG
AspHI 818 GAGCAC G[AT]GC[AT]^C
1255 GTGCTC
BalI 2017 TGGCCA TGG^CCA
2362 TGGCCA
BamHI 20 GGATCC G^GATCC
626 GGATCC
BlpI 1547 GCTCAGC GC^T[ACGT]AGC
2227 GCTAAGC
Bpu1102I 1547 GCTCAGC GC^T[ACGT]AGC
2227 GCTAAGC
BsiHKAI 818 GAGCAC G[AT]GC[AT]^C
1255 GTGCTC
Bsp1407I 914 TGTACA T^GTACA
2874 TGTACA
BsrGI 914 TGTACA T^GTACA
2874 TGTACA
BstAPI 279 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1843 GCACCTTCTGC
BstYI 20 GGATCC [GA]^GATC[CT]
626 GGATCC
Bsu36I 265 CCTCAGG CC^T[ACGT]AGG
2514 CCTAAGG
CelII 1547 GCTCAGC GC^T[ACGT]AGC
2227 GCTAAGC
DraIII 2667 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
3091 CACTTTGTG
DrdI 117 GACCGCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
541 GACGCGCTCGTC
Eco81I 265 CCTCAGG CC^T[ACGT]AGG
2514 CCTAAGG
EcoT14I 621 CCATGG C^C[AT][AT]GG
2011 CCTTGG
MflI 20 GGATCC [GA]^GATC[CT]
626 GGATCC
MluNI 2017 TGGCCA TGG^CCA
2362 TGGCCA
MscI 2017 TGGCCA TGG^CCA
2362 TGGCCA
PflMI 1110 CCATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2885 CCAGTATTTGG
PpuMI 587 GGGTCCT [GA]G^G[AT]CC[CT]
2322 AGGTCCC
SapI 576 GCTCTTC GCTCTTC(1/4)
1802 GCTCTTC
SspBI 914 TGTACA T^GTACA
2874 TGTACA
StyI 621 CCATGG C^C[AT][AT]GG
2011 CCTTGG
TaqI 536 TCGA T^CGA
1530 TCGA
TthHB8I 536 TCGA T^CGA
1530 TCGA
Van91I 1110 CCATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2885 CCAGTATTTGG
XcmI 77 CCAGCGAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1110 CCATAAAGTGGATGG
XhoII 20 GGATCC [GA]^GATC[CT]
626 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AvaI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfoI SgrAI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI