################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AocI Aor51HI ApaI AscI Asp718I AviII AvrII BamHI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmBI BspDI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI FspI KpnI KspI MamI MfeI MunI NotI NruI NspV PacI PinAI Psp1406I PvuI SacII SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccIII 1495 TCCGGA T^CCGGA AhdI 2315 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 3433 ATTAAT AT^TAAT AsnI 3433 ATTAAT AT^TAAT AspEI 2315 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1418 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3042 TGGCCA TGG^CCA BanI 1541 GGCGCC G^G[CT][GA]CC BbeI 1541 GGCGCC GGCGC^C BbrPI 2272 CACGTG CAC^GTG BglII 777 AGATCT A^GATCT BsaAI 2272 CACGTG [CT]AC^GT[GA] BseAI 1495 TCCGGA T^CCGGA BsmI 1308 GCATTC GAATGC(1/-1) BspEI 1495 TCCGGA T^CCGGA BspHI 2471 TCATGA T^CATGA BspLU11I 969 ACATGT A^CATGT BsrFI 539 GCCGGC [GA]^CCGG[CT] BssHII 27 GCGCGC G^CGCGC BstEII 936 GGTCACC G^GT[ACGT]ACC BstPI 936 GGTCACC G^GT[ACGT]ACC BstXI 1545 CCATGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 539 GCCGGC [GA]^CCGG[CT] CpoI 58 CGGACCG CG^G[AT]CCG EaeI 3042 TGGCCA [CT]^GGCC[GA] Eam1105I 2315 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 373 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 936 GGTCACC G^GT[ACGT]ACC EcoRI 2628 GAATTC G^AATTC HindIII 2187 AAGCTT A^AGCTT HpaI 1622 GTTAAC GTT^AAC KasI 1541 GGCGCC G^GCGCC MluI 453 ACGCGT A^CGCGT MluNI 3042 TGGCCA TGG^CCA MroI 1495 TCCGGA T^CCGGA MscI 3042 TGGCCA TGG^CCA NaeI 539 GCCGGC GCC^GGC NarI 1541 GGCGCC GG^CGCC NdeI 2115 CATATG CA^TATG NgoMIV 539 GCCGGC G^CCGGC NheI 2512 GCTAGC G^CTAGC PaeR7I 1805 CTCGAG C^TCGAG PflFI 1418 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2272 CACGTG CAC^GTG PmeI 2207 GTTTAAAC GTTT^AAAC PmlI 2272 CACGTG CAC^GTG PpuMI 2984 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 1074 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3433 ATTAAT AT^TAAT RcaI 2471 TCATGA T^CATGA RsrII 58 CGGACCG CG^G[AT]CCG SacI 1031 GAGCTC GAGCT^C SalI 2323 GTCGAC G^TCGAC SapI 2516 GCTCTTC GCTCTTC(1/4) SfoI 1541 GGCGCC GGC^GCC SspI 1739 AATATT AAT^ATT StuI 156 AGGCCT AGG^CCT Tth111I 1418 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3201 TCTAGA T^CTAGA XcmI 2287 CCACCTGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1805 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 2323 GTCGAC GT^[AC][GT]AC 3088 GTAGAC Alw44I 952 GTGCAC G^TGCAC 2269 GTGCAC ApaLI 952 GTGCAC G^TGCAC 2269 GTGCAC AvaI 45 CTCGGG C^[CT]CG[GA]G 1805 CTCGAG BbsI 686 GAAGAC GAAGAC(2/6) 2016 GTCTTC BcgI 1170 CGAAAGAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 258 GCATCAGCATCG BpuAI 686 GAAGAC GAAGAC(2/6) 2016 GTCTTC BsoBI 45 CTCGGG C^[CT]CG[GA]G 1805 CTCGAG Bsp1407I 1645 TGTACA T^GTACA 2645 TGTACA BsrBI 55 GAGCGG CCGCTC(-3/-3) 1987 GAGCGG BsrDI 3221 CATTGC GCAATG(2/0) 2494 GCAATG BsrGI 1645 TGTACA T^GTACA 2645 TGTACA EcoT22I 1615 ATGCAT ATGCA^T 2650 ATGCAT FauI 442 CCCGC CCCGC(4/6) 797 GCGGG NsiI 1615 ATGCAT ATGCA^T 2650 ATGCAT PflMI 104 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1405 CCAGCTCCTGG SspBI 1645 TGTACA T^GTACA 2645 TGTACA Van91I 104 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1405 CCAGCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanII BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI