##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AocI        Aor51HI     ApaI        AscI        Asp718I     
AviII       AvrII       BamHI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsiEI       BsiWI       BsmBI       BspDI       BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
CelII       ClaI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
FbaI        FseI        FspI        KpnI        KspI        
MamI        MfeI        MunI        NotI        NruI        
NspV        PacI        PinAI       Psp1406I    PvuI        
SacII       SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccIII       1495  TCCGGA               T^CCGGA
AhdI         2315  GACTTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         3433  ATTAAT               AT^TAAT
AsnI         3433  ATTAAT               AT^TAAT
AspEI        2315  GACTTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1418  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3042  TGGCCA               TGG^CCA
BanI         1541  GGCGCC               G^G[CT][GA]CC
BbeI         1541  GGCGCC               GGCGC^C
BbrPI        2272  CACGTG               CAC^GTG
BglII         777  AGATCT               A^GATCT
BsaAI        2272  CACGTG               [CT]AC^GT[GA]
BseAI        1495  TCCGGA               T^CCGGA
BsmI         1308  GCATTC               GAATGC(1/-1)
BspEI        1495  TCCGGA               T^CCGGA
BspHI        2471  TCATGA               T^CATGA
BspLU11I      969  ACATGT               A^CATGT
BsrFI         539  GCCGGC               [GA]^CCGG[CT]
BssHII         27  GCGCGC               G^CGCGC
BstEII        936  GGTCACC              G^GT[ACGT]ACC
BstPI         936  GGTCACC              G^GT[ACGT]ACC
BstXI        1545  CCATGGTCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        539  GCCGGC               [GA]^CCGG[CT]
CpoI           58  CGGACCG              CG^G[AT]CCG
EaeI         3042  TGGCCA               [CT]^GGCC[GA]
Eam1105I     2315  GACTTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         373  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       936  GGTCACC              G^GT[ACGT]ACC
EcoRI        2628  GAATTC               G^AATTC
HindIII      2187  AAGCTT               A^AGCTT
HpaI         1622  GTTAAC               GTT^AAC
KasI         1541  GGCGCC               G^GCGCC
MluI          453  ACGCGT               A^CGCGT
MluNI        3042  TGGCCA               TGG^CCA
MroI         1495  TCCGGA               T^CCGGA
MscI         3042  TGGCCA               TGG^CCA
NaeI          539  GCCGGC               GCC^GGC
NarI         1541  GGCGCC               GG^CGCC
NdeI         2115  CATATG               CA^TATG
NgoMIV        539  GCCGGC               G^CCGGC
NheI         2512  GCTAGC               G^CTAGC
PaeR7I       1805  CTCGAG               C^TCGAG
PflFI        1418  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2272  CACGTG               CAC^GTG
PmeI         2207  GTTTAAAC             GTTT^AAAC
PmlI         2272  CACGTG               CAC^GTG
PpuMI        2984  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1074  GACAGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3433  ATTAAT               AT^TAAT
RcaI         2471  TCATGA               T^CATGA
RsrII          58  CGGACCG              CG^G[AT]CCG
SacI         1031  GAGCTC               GAGCT^C
SalI         2323  GTCGAC               G^TCGAC
SapI         2516  GCTCTTC              GCTCTTC(1/4)
SfoI         1541  GGCGCC               GGC^GCC
SspI         1739  AATATT               AAT^ATT
StuI          156  AGGCCT               AGG^CCT
Tth111I      1418  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3201  TCTAGA               T^CTAGA
XcmI         2287  CCACCTGAGTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1805  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI         2323  GTCGAC               GT^[AC][GT]AC
             3088  GTAGAC              
Alw44I        952  GTGCAC               G^TGCAC
             2269  GTGCAC              
ApaLI         952  GTGCAC               G^TGCAC
             2269  GTGCAC              
AvaI           45  CTCGGG               C^[CT]CG[GA]G
             1805  CTCGAG              
BbsI          686  GAAGAC               GAAGAC(2/6)
             2016  GTCTTC              
BcgI         1170  CGAAAGAATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              258  GCATCAGCATCG        
BpuAI         686  GAAGAC               GAAGAC(2/6)
             2016  GTCTTC              
BsoBI          45  CTCGGG               C^[CT]CG[GA]G
             1805  CTCGAG              
Bsp1407I     1645  TGTACA               T^GTACA
             2645  TGTACA              
BsrBI          55  GAGCGG               CCGCTC(-3/-3)
             1987  GAGCGG              
BsrDI        3221  CATTGC               GCAATG(2/0)
             2494  GCAATG              
BsrGI        1645  TGTACA               T^GTACA
             2645  TGTACA              
EcoT22I      1615  ATGCAT               ATGCA^T
             2650  ATGCAT              
FauI          442  CCCGC                CCCGC(4/6)
              797  GCGGG               
NsiI         1615  ATGCAT               ATGCA^T
             2650  ATGCAT              
PflMI         104  CCAGAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1405  CCAGCTCCTGG         
SspBI        1645  TGTACA               T^GTACA
             2645  TGTACA              
Van91I        104  CCAGAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1405  CCAGCTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaII       BanII       BbvCI       
BbvI        BciVI       BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI