##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AocI Aor51HI ApaI AscI Asp718I
AviII AvrII BamHI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmBI BspDI BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRV
FbaI FseI FspI KpnI KspI
MamI MfeI MunI NotI NruI
NspV PacI PinAI Psp1406I PvuI
SacII SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccIII 1495 TCCGGA T^CCGGA
AhdI 2315 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3433 ATTAAT AT^TAAT
AsnI 3433 ATTAAT AT^TAAT
AspEI 2315 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1418 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3042 TGGCCA TGG^CCA
BanI 1541 GGCGCC G^G[CT][GA]CC
BbeI 1541 GGCGCC GGCGC^C
BbrPI 2272 CACGTG CAC^GTG
BglII 777 AGATCT A^GATCT
BsaAI 2272 CACGTG [CT]AC^GT[GA]
BseAI 1495 TCCGGA T^CCGGA
BsmI 1308 GCATTC GAATGC(1/-1)
BspEI 1495 TCCGGA T^CCGGA
BspHI 2471 TCATGA T^CATGA
BspLU11I 969 ACATGT A^CATGT
BsrFI 539 GCCGGC [GA]^CCGG[CT]
BssHII 27 GCGCGC G^CGCGC
BstEII 936 GGTCACC G^GT[ACGT]ACC
BstPI 936 GGTCACC G^GT[ACGT]ACC
BstXI 1545 CCATGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 539 GCCGGC [GA]^CCGG[CT]
CpoI 58 CGGACCG CG^G[AT]CCG
EaeI 3042 TGGCCA [CT]^GGCC[GA]
Eam1105I 2315 GACTTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 373 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 936 GGTCACC G^GT[ACGT]ACC
EcoRI 2628 GAATTC G^AATTC
HindIII 2187 AAGCTT A^AGCTT
HpaI 1622 GTTAAC GTT^AAC
KasI 1541 GGCGCC G^GCGCC
MluI 453 ACGCGT A^CGCGT
MluNI 3042 TGGCCA TGG^CCA
MroI 1495 TCCGGA T^CCGGA
MscI 3042 TGGCCA TGG^CCA
NaeI 539 GCCGGC GCC^GGC
NarI 1541 GGCGCC GG^CGCC
NdeI 2115 CATATG CA^TATG
NgoMIV 539 GCCGGC G^CCGGC
NheI 2512 GCTAGC G^CTAGC
PaeR7I 1805 CTCGAG C^TCGAG
PflFI 1418 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2272 CACGTG CAC^GTG
PmeI 2207 GTTTAAAC GTTT^AAAC
PmlI 2272 CACGTG CAC^GTG
PpuMI 2984 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1074 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3433 ATTAAT AT^TAAT
RcaI 2471 TCATGA T^CATGA
RsrII 58 CGGACCG CG^G[AT]CCG
SacI 1031 GAGCTC GAGCT^C
SalI 2323 GTCGAC G^TCGAC
SapI 2516 GCTCTTC GCTCTTC(1/4)
SfoI 1541 GGCGCC GGC^GCC
SspI 1739 AATATT AAT^ATT
StuI 156 AGGCCT AGG^CCT
Tth111I 1418 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3201 TCTAGA T^CTAGA
XcmI 2287 CCACCTGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1805 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2323 GTCGAC GT^[AC][GT]AC
3088 GTAGAC
Alw44I 952 GTGCAC G^TGCAC
2269 GTGCAC
ApaLI 952 GTGCAC G^TGCAC
2269 GTGCAC
AvaI 45 CTCGGG C^[CT]CG[GA]G
1805 CTCGAG
BbsI 686 GAAGAC GAAGAC(2/6)
2016 GTCTTC
BcgI 1170 CGAAAGAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
258 GCATCAGCATCG
BpuAI 686 GAAGAC GAAGAC(2/6)
2016 GTCTTC
BsoBI 45 CTCGGG C^[CT]CG[GA]G
1805 CTCGAG
Bsp1407I 1645 TGTACA T^GTACA
2645 TGTACA
BsrBI 55 GAGCGG CCGCTC(-3/-3)
1987 GAGCGG
BsrDI 3221 CATTGC GCAATG(2/0)
2494 GCAATG
BsrGI 1645 TGTACA T^GTACA
2645 TGTACA
EcoT22I 1615 ATGCAT ATGCA^T
2650 ATGCAT
FauI 442 CCCGC CCCGC(4/6)
797 GCGGG
NsiI 1615 ATGCAT ATGCA^T
2650 ATGCAT
PflMI 104 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1405 CCAGCTCCTGG
SspBI 1645 TGTACA T^GTACA
2645 TGTACA
Van91I 104 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1405 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanII BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI