##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI BbeI
BbrPI BclI BglI BlpI Bpu1102I
BsaAI BsaHI BsiEI BsiWI BspDI
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HaeII HgaI Hin1I KasI KpnI
KspI MluI NarI NheI NotI
NruI NsiI NspV PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1393 GTCTAC GT^[AC][GT]AC
AccII 87 CGCG CG^CG
AccIII 908 TCCGGA T^CCGGA
AcsI 1713 GAATTT [GA]^AATT[CT]
AflII 3159 CTTAAG C^TTAAG
AlwNI 2641 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1713 GAATTT [GA]^AATT[CT]
AvaI 691 CCCGAG C^[CT]CG[GA]G
AviII 1445 TGCGCA TGC^GCA
AvrII 3045 CCTAGG C^CTAGG
BcgI 3361 GCAGTGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3159 CTTAAG C^TTAAG
BlnI 3045 CCTAGG C^CTAGG
BseAI 908 TCCGGA T^CCGGA
BsmBI 1198 CGTCTC CGTCTC(1/5)
BsoBI 691 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1246 TGTACA T^GTACA
BspEI 908 TCCGGA T^CCGGA
BsrFI 353 GCCGGC [GA]^CCGG[CT]
BsrGI 1246 TGTACA T^GTACA
BstUI 87 CGCG CG^CG
Cfr10I 353 GCCGGC [GA]^CCGG[CT]
DraI 3541 TTTAAA TTT^AAA
DraIII 3376 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 247 GACCGGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1445 TGCGCA TGC^GCA
HpaI 1774 GTTAAC GTT^AAC
MfeI 3600 CAATTG C^AATTG
MroI 908 TCCGGA T^CCGGA
MunI 3600 CAATTG C^AATTG
MvnI 87 CGCG CG^CG
NaeI 353 GCCGGC GCC^GGC
NdeI 1430 CATATG CA^TATG
NgoMIV 353 GCCGGC G^CCGGC
PacI 3562 TTAATTAA TTAAT^TAA
SapI 1062 GCTCTTC GCTCTTC(1/4)
ScaI 170 AGTACT AGT^ACT
SspBI 1246 TGTACA T^GTACA
XcmI 3175 CCAAACTCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 763 GTGCAC G^TGCAC
3340 GTGCAC
ApaI 1950 GGGCCC GGGCC^C
3311 GGGCCC
ApaLI 763 GTGCAC G^TGCAC
3340 GTGCAC
Asp700I 1296 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1584 GAACCAGTTC
BbvCI 891 CCTCAGC CCTCAGC(-5/-2)
1032 CCTCAGC
BciVI 2133 GGATAC GTATCC(6/5)
1260 GTATCC
BglII 530 AGATCT A^GATCT
2850 AGATCT
BsaBI 900 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1080 GATTGTCATC
BsaI 2634 GAGACC GGTCTC(1/5)
3171 GAGACC
BsaWI 248 ACCGGA [AT]^CCGG[AT]
908 TCCGGA
BsmI 1055 GCATTC GAATGC(1/-1)
1738 GCATTC
BspHI 278 TCATGA T^CATGA
1136 TCATGA
BspMI 410 ACCTGC ACCTGC(4/8)
536 ACCTGC
BsrDI 179 CATTGC GCAATG(2/0)
399 CATTGC
BssSI 2016 CTCGTG CACGAG(-5/-1)
2652 CTCGTG
CfoI 88 GCGC GCG^C
1446 GCGC
DsaI 965 CCATGG C^C[GA][CT]GG
1346 CCATGG
HhaI 88 GCGC GCG^C
1446 GCGC
HinP1I 88 GCGC G^CGC
1446 GCGC
MaeII 389 ACGT A^CGT
1745 ACGT
MamI 900 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1080 GATTGTCATC
NcoI 965 CCATGG C^CATGG
1346 CCATGG
PpuMI 653 AGGACCT [GA]G^G[AT]CC[CT]
2929 GGGTCCT
PstI 1992 CTGCAG CTGCA^G
2413 CTGCAG
PvuII 742 CAGCTG CAG^CTG
1678 CAGCTG
RcaI 278 TCATGA T^CATGA
1136 TCATGA
SacI 433 GAGCTC GAGCT^C
1130 GAGCTC
XmnI 1296 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1584 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII