##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BcgI BclI BglII BlnI
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BssSI Bst1107I BstBI BstZ17I
ClaI DraIII EagI Eam1105I EclXI
Eco52I EcoRI FbaI FseI FspI
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NdeI NotI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI PvuI
SacI SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 1079 AACGTT AA^CGTT
AspI 40 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1477 TGGCCA TGG^CCA
BbrPI 1881 CACGTG CAC^GTG
BsaAI 1881 CACGTG [CT]AC^GT[GA]
BsaWI 4 TCCGGT [AT]^CCGG[AT]
BsmBI 3252 CGTCTC CGTCTC(1/5)
BspHI 1164 TCATGA T^CATGA
BsrBI 111 CCGCTC CCGCTC(-3/-3)
BsrDI 3181 CATTGC GCAATG(2/0)
BssHII 24 GCGCGC G^CGCGC
BstEII 137 GGTGACC G^GT[ACGT]ACC
BstPI 137 GGTGACC G^GT[ACGT]ACC
BstXI 1707 CCATCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 11 CGGTCCG CG^G[AT]CCG
DraI 3588 TTTAAA TTT^AAA
EcoO65I 137 GGTGACC G^GT[ACGT]ACC
EcoT22I 978 ATGCAT ATGCA^T
MluNI 1477 TGGCCA TGG^CCA
MscI 1477 TGGCCA TGG^CCA
NaeI 1610 GCCGGC GCC^GGC
NgoMIV 1610 GCCGGC G^CCGGC
NheI 2302 GCTAGC G^CTAGC
NruI 226 TCGCGA TCG^CGA
NsiI 978 ATGCAT ATGCA^T
PflFI 40 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1881 CACGTG CAC^GTG
PmlI 1881 CACGTG CAC^GTG
Psp1406I 1079 AACGTT AA^CGTT
RcaI 1164 TCATGA T^CATGA
RsrII 11 CGGTCCG CG^G[AT]CCG
SbfI 2608 CCTGCAGG CCTGCA^GG
SexAI 1918 ACCAGGT A^CC[AT]GGT
SphI 2333 GCATGC GCATG^C
Sse8387I 2608 CCTGCAGG CCTGCA^GG
SspI 1374 AATATT AAT^ATT
StuI 1252 AGGCCT AGG^CCT
Tth111I 40 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2822 CCAGTTTGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1982 GTCTAC GT^[AC][GT]AC
3323 GTCTAC
AcyI 989 GGCGTC G[GA]^CG[CT]C
1280 GACGCC
AflII 1605 CTTAAG C^TTAAG
1829 CTTAAG
AflIII 1732 ACATGT A^C[GA][CT]GT
3319 ACATGT
BanI 728 GGCACC G^G[CT][GA]CC
1886 GGCACC
BfrI 1605 CTTAAG C^TTAAG
1829 CTTAAG
BsaHI 989 GGCGTC G[GA]^CG[CT]C
1280 GACGCC
BspLU11I 1732 ACATGT A^CATGT
3319 ACATGT
BspMI 2406 GCAGGT ACCTGC(4/8)
2611 GCAGGT
BsrFI 156 ACCGGC [GA]^CCGG[CT]
1610 GCCGGC
BstYI 360 AGATCC [GA]^GATC[CT]
3509 GGATCT
Cfr10I 156 ACCGGC [GA]^CCGG[CT]
1610 GCCGGC
DrdI 1527 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1593 GACAAGTATGTC
EcoNI 1284 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1740 CCTGGCAAAGG
EcoRV 653 GATATC GAT^ATC
2634 GATATC
Hin1I 989 GGCGTC G[GA]^CG[CT]C
1280 GACGCC
HincII 799 GTTGAC GT[CT]^[GA]AC
3176 GTCAAC
HindII 799 GTTGAC GT[CT]^[GA]AC
3176 GTCAAC
KspI 142 CCGCGG CCGC^GG
1645 CCGCGG
MflI 360 AGATCC [GA]^GATC[CT]
3509 GGATCT
PflMI 42 CCATGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3476 CCAAGAATTGG
PpuMI 1764 GGGACCC [GA]G^G[AT]CC[CT]
2408 AGGTCCT
SacII 142 CCGCGG CCGC^GG
1645 CCGCGG
SapI 349 GAAGAGC GCTCTTC(1/4)
52 GCTCTTC
SmaI 119 CCCGGG CCC^GGG
1099 CCCGGG
Van91I 42 CCATGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3476 CCAAGAATTGG
XhoII 360 AGATCC [GA]^GATC[CT]
3509 GGATCT
XmaCI 119 CCCGGG C^CCGGG
1099 CCCGGG
XmaI 119 CCCGGG C^CCGGG
1099 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
ApoI Asp700I AspHI AvaI AvaII
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI