################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII AccIII AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AviII AvrII BalI BamHI BbrPI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BseAI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FbaI FseI FspI HpaI KspI MluI MluNI MroI MscI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### Acc65I 142 GGTACC G^GTACC AccI 562 GTCTAC GT^[AC][GT]AC AccII 129 CGCG CG^CG AclI 2916 AACGTT AA^CGTT AcyI 165 GGCGCC G[GA]^CG[CT]C AflIII 2466 ACACGT A^C[GA][CT]GT AhdI 988 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1531 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 2855 ATTAAT AT^TAAT AsnI 2855 ATTAAT AT^TAAT Asp718I 142 GGTACC G^GTACC AspEI 988 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 3131 GAGCTC G[AT]GC[AT]^C AspI 786 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1177 CCCGGG C^[CT]CG[GA]G BbeI 165 GGCGCC GGCGC^C BciVI 1445 GGATAC GTATCC(6/5) BglI 168 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 118 TACGTG [CT]AC^GT[GA] BsaBI 1963 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 165 GGCGCC G[GA]^CG[CT]C BsaWI 713 TCCGGT [AT]^CCGG[AT] BsiEI 687 CGACCG CG[GA][CT]^CG BsiHKAI 3131 GAGCTC G[AT]GC[AT]^C BsoBI 1177 CCCGGG C^[CT]CG[GA]G BspHI 2474 TCATGA T^CATGA BsrFI 101 ACCGGC [GA]^CCGG[CT] BssSI 2022 CACGAG CACGAG(-5/-1) BstAPI 3113 GCAAGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 129 CGCG CG^CG BstXI 2564 CCAGTTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 101 ACCGGC [GA]^CCGG[CT] DraII 11 GGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 1292 CCATGG C^C[GA][CT]GG Eam1105I 988 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 11 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 1152 GAATTC G^AATTC EcoT22I 1559 ATGCAT ATGCA^T Hin1I 165 GGCGCC G[GA]^CG[CT]C HindIII 2668 AAGCTT A^AGCTT HphI 2327 GGTGA GGTGA(8/7) KasI 165 GGCGCC G^GCGCC KpnI 142 GGTACC GGTAC^C MamI 1963 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2823 CAATTG C^AATTG MunI 2823 CAATTG C^AATTG MvnI 129 CGCG CG^CG NarI 165 GGCGCC GG^CGCC NcoI 1292 CCATGG C^CATGG NdeI 2108 CATATG CA^TATG NheI 973 GCTAGC G^CTAGC NsiI 1559 ATGCAT ATGCA^T PflFI 786 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 11 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 2855 ATTAAT AT^TAAT Psp1406I 2916 AACGTT AA^CGTT RcaI 2474 TCATGA T^CATGA SacI 3131 GAGCTC GAGCT^C SapI 805 GAAGAGC GCTCTTC(1/4) SfoI 165 GGCGCC GGC^GCC SmaI 1177 CCCGGG CCC^GGG SmlI 2993 CTCAAG C^T[CT][GA]AG SspI 1386 AATATT AAT^ATT SwaI 2797 ATTTAAAT ATTT^AAAT Tth111I 786 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1177 CCCGGG C^CCGGG XmaI 1177 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 1152 GAATTC [GA]^AATT[CT] 2874 GAATTT ApoI 1152 GAATTC [GA]^AATT[CT] 2874 GAATTT BanII 1103 GGGCTC G[GA]GC[CT]^C 3131 GAGCTC BbvCI 135 CCTCAGC CCTCAGC(-5/-2) 1440 GCTGAGG BpmI 674 CTGGAG CTGGAG(16/14) 3264 CTGGAG BsmI 2361 GAATGC GAATGC(1/-1) 3186 GAATGC Bsp1407I 2722 TGTACA T^GTACA 3277 TGTACA BsrGI 2722 TGTACA T^GTACA 3277 TGTACA BstYI 1968 AGATCC [GA]^GATC[CT] 2560 AGATCC DrdI 763 GACGAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2250 GACATGAGTGTC EcoNI 1353 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3137 CCTTCTGCAGG HaeII 165 GGCGCC [GA]GCGC^[CT] 1280 AGCGCC HgaI 473 GCGTC GACGC(5/10) 2613 GCGTC HincII 778 GTTGAC GT[CT]^[GA]AC 2121 GTTGAC HindII 778 GTTGAC GT[CT]^[GA]AC 2121 GTTGAC MflI 1968 AGATCC [GA]^GATC[CT] 2560 AGATCC MslI 428 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2194 CATTCAAATG PflMI 1614 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2261 CCAATATATGG ScaI 1258 AGTACT AGT^ACT 3089 AGTACT SspBI 2722 TGTACA T^GTACA 3277 TGTACA StuI 657 AGGCCT AGG^CCT 2028 AGGCCT Van91I 1614 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2261 CCAATATATGG XcmI 636 CCAGCCTTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1034 CCATTGAGCGAGTGG XhoII 1968 AGATCC [GA]^GATC[CT] 2560 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanI BbsI BbvI BcnI BfaI BmyI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I