##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I AviII AvrII BalI BamHI
BbrPI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI FspI HpaI KspI MluI
MluNI MroI MscI NaeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 142 GGTACC G^GTACC
AccI 562 GTCTAC GT^[AC][GT]AC
AccII 129 CGCG CG^CG
AclI 2916 AACGTT AA^CGTT
AcyI 165 GGCGCC G[GA]^CG[CT]C
AflIII 2466 ACACGT A^C[GA][CT]GT
AhdI 988 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1531 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2855 ATTAAT AT^TAAT
AsnI 2855 ATTAAT AT^TAAT
Asp718I 142 GGTACC G^GTACC
AspEI 988 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3131 GAGCTC G[AT]GC[AT]^C
AspI 786 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1177 CCCGGG C^[CT]CG[GA]G
BbeI 165 GGCGCC GGCGC^C
BciVI 1445 GGATAC GTATCC(6/5)
BglI 168 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 118 TACGTG [CT]AC^GT[GA]
BsaBI 1963 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 165 GGCGCC G[GA]^CG[CT]C
BsaWI 713 TCCGGT [AT]^CCGG[AT]
BsiEI 687 CGACCG CG[GA][CT]^CG
BsiHKAI 3131 GAGCTC G[AT]GC[AT]^C
BsoBI 1177 CCCGGG C^[CT]CG[GA]G
BspHI 2474 TCATGA T^CATGA
BsrFI 101 ACCGGC [GA]^CCGG[CT]
BssSI 2022 CACGAG CACGAG(-5/-1)
BstAPI 3113 GCAAGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 129 CGCG CG^CG
BstXI 2564 CCAGTTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 101 ACCGGC [GA]^CCGG[CT]
DraII 11 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 1292 CCATGG C^C[GA][CT]GG
Eam1105I 988 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 11 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1152 GAATTC G^AATTC
EcoT22I 1559 ATGCAT ATGCA^T
Hin1I 165 GGCGCC G[GA]^CG[CT]C
HindIII 2668 AAGCTT A^AGCTT
HphI 2327 GGTGA GGTGA(8/7)
KasI 165 GGCGCC G^GCGCC
KpnI 142 GGTACC GGTAC^C
MamI 1963 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2823 CAATTG C^AATTG
MunI 2823 CAATTG C^AATTG
MvnI 129 CGCG CG^CG
NarI 165 GGCGCC GG^CGCC
NcoI 1292 CCATGG C^CATGG
NdeI 2108 CATATG CA^TATG
NheI 973 GCTAGC G^CTAGC
NsiI 1559 ATGCAT ATGCA^T
PflFI 786 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 11 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2855 ATTAAT AT^TAAT
Psp1406I 2916 AACGTT AA^CGTT
RcaI 2474 TCATGA T^CATGA
SacI 3131 GAGCTC GAGCT^C
SapI 805 GAAGAGC GCTCTTC(1/4)
SfoI 165 GGCGCC GGC^GCC
SmaI 1177 CCCGGG CCC^GGG
SmlI 2993 CTCAAG C^T[CT][GA]AG
SspI 1386 AATATT AAT^ATT
SwaI 2797 ATTTAAAT ATTT^AAAT
Tth111I 786 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1177 CCCGGG C^CCGGG
XmaI 1177 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1152 GAATTC [GA]^AATT[CT]
2874 GAATTT
ApoI 1152 GAATTC [GA]^AATT[CT]
2874 GAATTT
BanII 1103 GGGCTC G[GA]GC[CT]^C
3131 GAGCTC
BbvCI 135 CCTCAGC CCTCAGC(-5/-2)
1440 GCTGAGG
BpmI 674 CTGGAG CTGGAG(16/14)
3264 CTGGAG
BsmI 2361 GAATGC GAATGC(1/-1)
3186 GAATGC
Bsp1407I 2722 TGTACA T^GTACA
3277 TGTACA
BsrGI 2722 TGTACA T^GTACA
3277 TGTACA
BstYI 1968 AGATCC [GA]^GATC[CT]
2560 AGATCC
DrdI 763 GACGAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2250 GACATGAGTGTC
EcoNI 1353 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3137 CCTTCTGCAGG
HaeII 165 GGCGCC [GA]GCGC^[CT]
1280 AGCGCC
HgaI 473 GCGTC GACGC(5/10)
2613 GCGTC
HincII 778 GTTGAC GT[CT]^[GA]AC
2121 GTTGAC
HindII 778 GTTGAC GT[CT]^[GA]AC
2121 GTTGAC
MflI 1968 AGATCC [GA]^GATC[CT]
2560 AGATCC
MslI 428 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2194 CATTCAAATG
PflMI 1614 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2261 CCAATATATGG
ScaI 1258 AGTACT AGT^ACT
3089 AGTACT
SspBI 2722 TGTACA T^GTACA
3277 TGTACA
StuI 657 AGGCCT AGG^CCT
2028 AGGCCT
Van91I 1614 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2261 CCAATATATGG
XcmI 636 CCAGCCTTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1034 CCATTGAGCGAGTGG
XhoII 1968 AGATCC [GA]^GATC[CT]
2560 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I