##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AspI AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI HaeII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NheI NotI NruI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2243 GACGTC GACGT^C
AflIII 1146 ACACGT A^C[GA][CT]GT
AhdI 320 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2505 GTGCAC G^TGCAC
ApaI 2556 GGGCCC GGGCC^C
ApaLI 2505 GTGCAC G^TGCAC
Asp700I 536 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 320 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 453 TGCGCA TGC^GCA
BalI 1446 TGGCCA TGG^CCA
BanI 1159 GGTGCC G^G[CT][GA]CC
BbvCI 1748 GCTGAGG CCTCAGC(-5/-2)
BsaAI 1147 CACGTA [CT]AC^GT[GA]
BsiEI 150 CGGTCG CG[GA][CT]^CG
BsmBI 2249 CGTCTC CGTCTC(1/5)
BspMI 648 ACCTGC ACCTGC(4/8)
BsrFI 2338 GCCGGC [GA]^CCGG[CT]
BstAPI 1231 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1279 GGTCACC G^GT[ACGT]ACC
BstPI 1279 GGTCACC G^GT[ACGT]ACC
Cfr10I 2338 GCCGGC [GA]^CCGG[CT]
DrdI 2616 GACGTTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 320 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1279 GGTCACC G^GT[ACGT]ACC
FspI 453 TGCGCA TGC^GCA
HincII 1505 GTCAAC GT[CT]^[GA]AC
HindII 1505 GTCAAC GT[CT]^[GA]AC
HindIII 2751 AAGCTT A^AGCTT
MluNI 1446 TGGCCA TGG^CCA
MscI 1446 TGGCCA TGG^CCA
NaeI 2338 GCCGGC GCC^GGC
NdeI 993 CATATG CA^TATG
NgoMIV 2338 GCCGGC G^CCGGC
PaeR7I 431 CTCGAG C^TCGAG
PpuMI 2406 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2243 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1562 CAGCTG CAG^CTG
SacI 2400 GAGCTC GAGCT^C
SexAI 1464 ACCAGGT A^CC[AT]GGT
SmaI 96 CCCGGG CCC^GGG
XbaI 2626 TCTAGA T^CTAGA
XhoI 431 CTCGAG C^TCGAG
XmaCI 96 CCCGGG C^CCGGG
XmaI 96 CCCGGG C^CCGGG
XmnI 536 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 110 CGCG CG^CG
557 CGCG
AcyI 1319 GACGCC G[GA]^CG[CT]C
2243 GACGTC
AocI 1187 CCTGAGG CC^T[ACGT]AGG
1403 CCTGAGG
AvaI 96 CCCGGG C^[CT]CG[GA]G
431 CTCGAG
BcnI 96 CCCGG CC^[GC]GG
97 CCGGG
BglII 220 AGATCT A^GATCT
507 AGATCT
BpmI 133 CTCCAG CTGGAG(16/14)
2643 CTCCAG
BsaHI 1319 GACGCC G[GA]^CG[CT]C
2243 GACGTC
BsgI 58 CTGCAC GTGCAG(16/14)
1433 CTGCAC
BsmI 540 GCATTC GAATGC(1/-1)
2342 GCATTC
BsoBI 96 CCCGGG C^[CT]CG[GA]G
431 CTCGAG
BsrDI 263 GCAATG GCAATG(2/0)
1665 GCAATG
BstUI 110 CGCG CG^CG
557 CGCG
BstXI 4 CCACTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2320 CCACCGTGATGG
Bsu36I 1187 CCTGAGG CC^T[ACGT]AGG
1403 CCTGAGG
DraIII 572 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2586 CACTGTGTG
Eco81I 1187 CCTGAGG CC^T[ACGT]AGG
1403 CCTGAGG
EcoNI 285 CCTTGTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
406 CCTCCGAGAGG
EcoT22I 990 ATGCAT ATGCA^T
1168 ATGCAT
HapII 97 CCGG C^CGG
2339 CCGG
HgaI 741 GACGC GACGC(5/10)
1319 GACGC
Hin1I 1319 GACGCC G[GA]^CG[CT]C
2243 GACGTC
HpaII 97 CCGG C^CGG
2339 CCGG
MspI 97 CCGG C^CGG
2339 CCGG
MvnI 110 CGCG CG^CG
557 CGCG
NciI 96 CCCGG CC^[GC]GG
97 CCGGG
NsiI 990 ATGCAT ATGCA^T
1168 ATGCAT
SspI 893 AATATT AAT^ATT
970 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BfaI BmyI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII