##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AccII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbrPI BcgI BclI BfrI
BlnI BsaAI BsaBI BsiEI BsiWI
BsmBI BsmI BspDI BspHI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI HpaI KspI MamI MfeI
MluI MunI MvnI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SspI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 1338 TCCGGA T^CCGGA
AcsI 1546 GAATTC [GA]^AATT[CT]
AcyI 498 GGCGCC G[GA]^CG[CT]C
Alw44I 1216 GTGCAC G^TGCAC
AocI 1816 CCTGAGG CC^T[ACGT]AGG
ApaLI 1216 GTGCAC G^TGCAC
ApoI 1546 GAATTC [GA]^AATT[CT]
Asp700I 2274 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 498 GGCGCC GGCGC^C
BbvCI 1996 GCTGAGG CCTCAGC(-5/-2)
BglII 2370 AGATCT A^GATCT
BlpI 2136 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2136 GCTTAGC GC^T[ACGT]AGC
BsaHI 498 GGCGCC G[GA]^CG[CT]C
BsaI 151 GAGACC GGTCTC(1/5)
BsaWI 1338 TCCGGA [AT]^CCGG[AT]
BseAI 1338 TCCGGA T^CCGGA
Bsp1407I 624 TGTACA T^GTACA
BspEI 1338 TCCGGA T^CCGGA
BspMI 1895 ACCTGC ACCTGC(4/8)
BsrBI 479 GAGCGG CCGCTC(-3/-3)
BsrDI 1077 CATTGC GCAATG(2/0)
BsrGI 624 TGTACA T^GTACA
BstAPI 362 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1816 CCTGAGG CC^T[ACGT]AGG
CelII 2136 GCTTAGC GC^T[ACGT]AGC
CfoI 499 GCGC GCG^C
Eco81I 1816 CCTGAGG CC^T[ACGT]AGG
EcoRI 1546 GAATTC G^AATTC
HaeII 498 GGCGCC [GA]GCGC^[CT]
HhaI 499 GCGC GCG^C
Hin1I 498 GGCGCC G[GA]^CG[CT]C
HinP1I 499 GCGC G^CGC
HincII 853 GTCAAC GT[CT]^[GA]AC
HindII 853 GTCAAC GT[CT]^[GA]AC
HindIII 171 AAGCTT A^AGCTT
HphI 1321 GGTGA GGTGA(8/7)
KasI 498 GGCGCC G^GCGCC
MroI 1338 TCCGGA T^CCGGA
NaeI 1430 GCCGGC GCC^GGC
NarI 498 GGCGCC GG^CGCC
NgoMIV 1430 GCCGGC G^CCGGC
SacI 809 GAGCTC GAGCT^C
SapI 958 GAAGAGC GCTCTTC(1/4)
SfoI 498 GGCGCC GGC^GCC
SmaI 2441 CCCGGG CCC^GGG
SpeI 928 ACTAGT A^CTAGT
SspBI 624 TGTACA T^GTACA
XmaCI 2441 CCCGGG C^CCGGG
XmaI 2441 CCCGGG C^CCGGG
XmnI 2274 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 1065 GGTACC G^GTACC
1692 GGTACC
AflIII 790 ACATGT A^C[GA][CT]GT
1650 ACATGT
ApaI 979 GGGCCC GGGCC^C
1822 GGGCCC
Asp718I 1065 GGTACC G^GTACC
1692 GGTACC
AvaI 462 CTCGGG C^[CT]CG[GA]G
2441 CCCGGG
BalI 1454 TGGCCA TGG^CCA
1487 TGGCCA
BciVI 93 GGATAC GTATCC(6/5)
530 GTATCC
BglI 1447 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1480 GCCATCATGGC
BsoBI 462 CTCGGG C^[CT]CG[GA]G
2441 CCCGGG
BspLU11I 790 ACATGT A^CATGT
1650 ACATGT
BsrFI 1430 GCCGGC [GA]^CCGG[CT]
1967 ACCGGC
Cfr10I 1430 GCCGGC [GA]^CCGG[CT]
1967 ACCGGC
KpnI 1065 GGTACC GGTAC^C
1692 GGTACC
MaeII 311 ACGT A^CGT
2362 ACGT
MluNI 1454 TGGCCA TGG^CCA
1487 TGGCCA
MscI 1454 TGGCCA TGG^CCA
1487 TGGCCA
MseI 1394 TTAA T^TAA
2528 TTAA
PpuMI 970 GGGTCCC [GA]G^G[AT]CC[CT]
2406 AGGACCC
PstI 1834 CTGCAG CTGCA^G
2487 CTGCAG
Tru9I 1394 TTAA T^TAA
2528 TTAA
Tsp45I 1530 GTCAC ^GT[GC]AC
2027 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TfiI
TseI Tsp509I TspRI Van91I XhoII