##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AocI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII BamHI BbeI
BbrPI BfrI BsaAI BsaBI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BstAPI Bsu36I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
MamI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Alw44I 2804 GTGCAC G^TGCAC
Aor51HI 667 AGCGCT AGC^GCT
ApaLI 2804 GTGCAC G^TGCAC
Asp700I 2303 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 120 CCTAGG C^CTAGG
BbvCI 1424 GCTGAGG CCTCAGC(-5/-2)
BcgI 266 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1273 GTATCC GTATCC(6/5)
BclI 2443 TGATCA T^GATCA
BglI 859 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2434 AGATCT A^GATCT
BlnI 120 CCTAGG C^CTAGG
BlpI 1085 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1085 GCTGAGC GC^T[ACGT]AGC
BsmBI 193 GAGACG CGTCTC(1/5)
BsrBI 1974 CCGCTC CCGCTC(-3/-3)
BssHII 64 GCGCGC G^CGCGC
BssSI 951 CTCGTG CACGAG(-5/-1)
BstBI 1393 TTCGAA TT^CGAA
CelII 1085 GCTGAGC GC^T[ACGT]AGC
DraIII 630 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2748 GACCTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 667 AGCGCT AGC^GCT
FbaI 2443 TGATCA T^GATCA
HincII 2736 GTTGAC GT[CT]^[GA]AC
HindII 2736 GTTGAC GT[CT]^[GA]AC
HindIII 2451 AAGCTT A^AGCTT
KspI 53 CCGCGG CCGC^GG
MaeII 1002 ACGT A^CGT
NcoI 145 CCATGG C^CATGG
NspV 1393 TTCGAA TT^CGAA
PaeR7I 2174 CTCGAG C^TCGAG
PpuMI 2679 GGGACCC [GA]G^G[AT]CC[CT]
SacII 53 CCGCGG CCGC^GG
SfuI 1393 TTCGAA TT^CGAA
Tsp509I 4 AATT ^AATT
XhoI 2174 CTCGAG C^TCGAG
XmnI 2303 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1218 GTATAC GT^[AC][GT]AC
1659 GTATAC
AcyI 1101 GACGCC G[GA]^CG[CT]C
1959 GGCGTC
AvaI 1108 CTCGGG C^[CT]CG[GA]G
2174 CTCGAG
BsaHI 1101 GACGCC G[GA]^CG[CT]C
1959 GGCGTC
BsoBI 1108 CTCGGG C^[CT]CG[GA]G
2174 CTCGAG
BsrDI 1907 CATTGC GCAATG(2/0)
496 GCAATG
Bst1107I 1218 GTATAC GTA^TAC
1659 GTATAC
BstEII 324 GGTGACC G^GT[ACGT]ACC
2569 GGTCACC
BstPI 324 GGTGACC G^GT[ACGT]ACC
2569 GGTCACC
BstZ17I 1218 GTATAC GTA^TAC
1659 GTATAC
EcoNI 1107 CCTCGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2396 CCTGCTCAAGG
EcoO65I 324 GGTGACC G^GT[ACGT]ACC
2569 GGTCACC
Hin1I 1101 GACGCC G[GA]^CG[CT]C
1959 GGCGTC
NaeI 481 GCCGGC GCC^GGC
751 GCCGGC
NgoMIV 481 GCCGGC G^CCGGC
751 GCCGGC
PvuII 1362 CAGCTG CAG^CTG
1422 CAGCTG
SapI 777 GCTCTTC GCTCTTC(1/4)
2006 GCTCTTC
SbfI 716 CCTGCAGG CCTGCA^GG
1748 CCTGCAGG
Sse8387I 716 CCTGCAGG CCTGCA^GG
1748 CCTGCAGG
XcmI 1912 CCAGGAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2058 CCACGCCGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspEI BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XhoII