##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AviII BamHI BbeI
BciVI BfrI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspEI BsrBI BsrGI Bst1107I BstBI
BstZ17I Bsu36I CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FseI FspI HaeII HincII HindII
HpaI KasI KpnI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 2461 GTCTAC GT^[AC][GT]AC
AcyI 1625 GACGCC G[GA]^CG[CT]C
AfaI 2121 GTAC GT^AC
Asp700I 1675 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2193 CCTAGG C^CTAGG
BbrPI 1808 CACGTG CAC^GTG
BbvCI 1112 GCTGAGG CCTCAGC(-5/-2)
BclI 975 TGATCA T^GATCA
BglII 2141 AGATCT A^GATCT
BlnI 2193 CCTAGG C^CTAGG
BlpI 1133 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1133 GCTGAGC GC^T[ACGT]AGC
BsaAI 1808 CACGTG [CT]AC^GT[GA]
BsaBI 2142 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1625 GACGCC G[GA]^CG[CT]C
BsgI 765 CTGCAC GTGCAG(16/14)
BspDI 1658 ATCGAT AT^CGAT
BspHI 1668 TCATGA T^CATGA
BsrFI 1396 GCCGGC [GA]^CCGG[CT]
BssSI 2 CACGAG CACGAG(-5/-1)
BstAPI 275 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2598 GGTAACC G^GT[ACGT]ACC
BstPI 2598 GGTAACC G^GT[ACGT]ACC
CelII 1133 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1396 GCCGGC [GA]^CCGG[CT]
ClaI 1658 ATCGAT AT^CGAT
DraI 1944 TTTAAA TTT^AAA
DraIII 1808 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2481 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 853 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2598 GGTAACC G^GT[ACGT]ACC
FbaI 975 TGATCA T^GATCA
Hin1I 1625 GACGCC G[GA]^CG[CT]C
MamI 2142 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1396 GCCGGC GCC^GGC
NdeI 812 CATATG CA^TATG
NgoMIV 1396 GCCGGC G^CCGGC
PmaCI 1808 CACGTG CAC^GTG
PmlI 1808 CACGTG CAC^GTG
RcaI 1668 TCATGA T^CATGA
RsaI 2121 GTAC GT^AC
SacI 2006 GAGCTC GAGCT^C
SapI 1364 GAAGAGC GCTCTTC(1/4)
SbfI 146 CCTGCAGG CCTGCA^GG
SexAI 2615 ACCAGGT A^CC[AT]GGT
Sse8387I 146 CCTGCAGG CCTGCA^GG
XbaI 1938 TCTAGA T^CTAGA
XmnI 1675 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 2302 GAATTC [GA]^AATT[CT]
2436 GAATTC
AflIII 429 ACATGT A^C[GA][CT]GT
2697 ACATGT
AhdI 670 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1984 GACTGAGGGTC
AlwNI 1026 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1980 CAGTGACTG
ApoI 2302 GAATTC [GA]^AATT[CT]
2436 GAATTC
AspEI 670 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1984 GACTGAGGGTC
AspHI 504 GAGCAC G[AT]GC[AT]^C
2006 GAGCTC
BanI 121 GGTGCC G^G[CT][GA]CC
1010 GGTGCC
BcnI 2381 CCGGG CC^[GC]GG
2756 CCGGG
BsiHKAI 504 GAGCAC G[AT]GC[AT]^C
2006 GAGCTC
BspLU11I 429 ACATGT A^CATGT
2697 ACATGT
BsrDI 187 CATTGC GCAATG(2/0)
1161 GCAATG
BssHII 95 GCGCGC G^CGCGC
1881 GCGCGC
Eam1105I 670 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1984 GACTGAGGGTC
EcoRI 2302 GAATTC G^AATTC
2436 GAATTC
FauI 158 CCCGC CCCGC(4/6)
1905 CCCGC
HindIII 614 AAGCTT A^AGCTT
777 AAGCTT
KspI 20 CCGCGG CCGC^GG
117 CCGCGG
MaeII 1809 ACGT A^CGT
2859 ACGT
NciI 2381 CCGGG CC^[GC]GG
2756 CCGGG
NcoI 171 CCATGG C^CATGG
936 CCATGG
PflMI 2290 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2695 CCACATGTTGG
PpuMI 1525 GGGTCCC [GA]G^G[AT]CC[CT]
2676 AGGTCCC
SacII 20 CCGCGG CCGC^GG
117 CCGCGG
TfiI 652 GATTC G^A[AT]TC
1066 GAATC
Van91I 2290 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2695 CCACATGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BalI BanII BbsI BbvI BcgI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII