################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII BamHI BbeI BciVI BfrI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BsrBI BsrGI Bst1107I BstBI BstZ17I Bsu36I CpoI EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 2461 GTCTAC GT^[AC][GT]AC AcyI 1625 GACGCC G[GA]^CG[CT]C AfaI 2121 GTAC GT^AC Asp700I 1675 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 2193 CCTAGG C^CTAGG BbrPI 1808 CACGTG CAC^GTG BbvCI 1112 GCTGAGG CCTCAGC(-5/-2) BclI 975 TGATCA T^GATCA BglII 2141 AGATCT A^GATCT BlnI 2193 CCTAGG C^CTAGG BlpI 1133 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1133 GCTGAGC GC^T[ACGT]AGC BsaAI 1808 CACGTG [CT]AC^GT[GA] BsaBI 2142 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1625 GACGCC G[GA]^CG[CT]C BsgI 765 CTGCAC GTGCAG(16/14) BspDI 1658 ATCGAT AT^CGAT BspHI 1668 TCATGA T^CATGA BsrFI 1396 GCCGGC [GA]^CCGG[CT] BssSI 2 CACGAG CACGAG(-5/-1) BstAPI 275 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2598 GGTAACC G^GT[ACGT]ACC BstPI 2598 GGTAACC G^GT[ACGT]ACC CelII 1133 GCTGAGC GC^T[ACGT]AGC Cfr10I 1396 GCCGGC [GA]^CCGG[CT] ClaI 1658 ATCGAT AT^CGAT DraI 1944 TTTAAA TTT^AAA DraIII 1808 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2481 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 853 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2598 GGTAACC G^GT[ACGT]ACC FbaI 975 TGATCA T^GATCA Hin1I 1625 GACGCC G[GA]^CG[CT]C MamI 2142 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1396 GCCGGC GCC^GGC NdeI 812 CATATG CA^TATG NgoMIV 1396 GCCGGC G^CCGGC PmaCI 1808 CACGTG CAC^GTG PmlI 1808 CACGTG CAC^GTG RcaI 1668 TCATGA T^CATGA RsaI 2121 GTAC GT^AC SacI 2006 GAGCTC GAGCT^C SapI 1364 GAAGAGC GCTCTTC(1/4) SbfI 146 CCTGCAGG CCTGCA^GG SexAI 2615 ACCAGGT A^CC[AT]GGT Sse8387I 146 CCTGCAGG CCTGCA^GG XbaI 1938 TCTAGA T^CTAGA XmnI 1675 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 2302 GAATTC [GA]^AATT[CT] 2436 GAATTC AflIII 429 ACATGT A^C[GA][CT]GT 2697 ACATGT AhdI 670 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1984 GACTGAGGGTC AlwNI 1026 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 1980 CAGTGACTG ApoI 2302 GAATTC [GA]^AATT[CT] 2436 GAATTC AspEI 670 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1984 GACTGAGGGTC AspHI 504 GAGCAC G[AT]GC[AT]^C 2006 GAGCTC BanI 121 GGTGCC G^G[CT][GA]CC 1010 GGTGCC BcnI 2381 CCGGG CC^[GC]GG 2756 CCGGG BsiHKAI 504 GAGCAC G[AT]GC[AT]^C 2006 GAGCTC BspLU11I 429 ACATGT A^CATGT 2697 ACATGT BsrDI 187 CATTGC GCAATG(2/0) 1161 GCAATG BssHII 95 GCGCGC G^CGCGC 1881 GCGCGC Eam1105I 670 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1984 GACTGAGGGTC EcoRI 2302 GAATTC G^AATTC 2436 GAATTC FauI 158 CCCGC CCCGC(4/6) 1905 CCCGC HindIII 614 AAGCTT A^AGCTT 777 AAGCTT KspI 20 CCGCGG CCGC^GG 117 CCGCGG MaeII 1809 ACGT A^CGT 2859 ACGT NciI 2381 CCGGG CC^[GC]GG 2756 CCGGG NcoI 171 CCATGG C^CATGG 936 CCATGG PflMI 2290 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2695 CCACATGTTGG PpuMI 1525 GGGTCCC [GA]G^G[AT]CC[CT] 2676 AGGTCCC SacII 20 CCGCGG CCGC^GG 117 CCGCGG TfiI 652 GATTC G^A[AT]TC 1066 GAATC Van91I 2290 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2695 CCACATGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AluI AlwI AvaII BalI BanII BbsI BbvI BcgI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII