##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AvaI        AviII       BamHI       BbeI        
BciVI       BfrI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspEI       BsrBI       BsrGI       Bst1107I    BstBI       
BstZ17I     Bsu36I      CpoI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       EcoT22I     
FseI        FspI        HaeII       HincII      HindII      
HpaI        KasI        KpnI        MfeI        MluI        
MroI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         2461  GTCTAC               GT^[AC][GT]AC
AcyI         1625  GACGCC               G[GA]^CG[CT]C
AfaI         2121  GTAC                 GT^AC
Asp700I      1675  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        2193  CCTAGG               C^CTAGG
BbrPI        1808  CACGTG               CAC^GTG
BbvCI        1112  GCTGAGG              CCTCAGC(-5/-2)
BclI          975  TGATCA               T^GATCA
BglII        2141  AGATCT               A^GATCT
BlnI         2193  CCTAGG               C^CTAGG
BlpI         1133  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1133  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1808  CACGTG               [CT]AC^GT[GA]
BsaBI        2142  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1625  GACGCC               G[GA]^CG[CT]C
BsgI          765  CTGCAC               GTGCAG(16/14)
BspDI        1658  ATCGAT               AT^CGAT
BspHI        1668  TCATGA               T^CATGA
BsrFI        1396  GCCGGC               [GA]^CCGG[CT]
BssSI           2  CACGAG               CACGAG(-5/-1)
BstAPI        275  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2598  GGTAACC              G^GT[ACGT]ACC
BstPI        2598  GGTAACC              G^GT[ACGT]ACC
CelII        1133  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1396  GCCGGC               [GA]^CCGG[CT]
ClaI         1658  ATCGAT               AT^CGAT
DraI         1944  TTTAAA               TTT^AAA
DraIII       1808  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2481  GACAGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         853  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2598  GGTAACC              G^GT[ACGT]ACC
FbaI          975  TGATCA               T^GATCA
Hin1I        1625  GACGCC               G[GA]^CG[CT]C
MamI         2142  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1396  GCCGGC               GCC^GGC
NdeI          812  CATATG               CA^TATG
NgoMIV       1396  GCCGGC               G^CCGGC
PmaCI        1808  CACGTG               CAC^GTG
PmlI         1808  CACGTG               CAC^GTG
RcaI         1668  TCATGA               T^CATGA
RsaI         2121  GTAC                 GT^AC
SacI         2006  GAGCTC               GAGCT^C
SapI         1364  GAAGAGC              GCTCTTC(1/4)
SbfI          146  CCTGCAGG             CCTGCA^GG
SexAI        2615  ACCAGGT              A^CC[AT]GGT
Sse8387I      146  CCTGCAGG             CCTGCA^GG
XbaI         1938  TCTAGA               T^CTAGA
XmnI         1675  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI         2302  GAATTC               [GA]^AATT[CT]
             2436  GAATTC              
AflIII        429  ACATGT               A^C[GA][CT]GT
             2697  ACATGT              
AhdI          670  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1984  GACTGAGGGTC         
AlwNI        1026  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1980  CAGTGACTG           
ApoI         2302  GAATTC               [GA]^AATT[CT]
             2436  GAATTC              
AspEI         670  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1984  GACTGAGGGTC         
AspHI         504  GAGCAC               G[AT]GC[AT]^C
             2006  GAGCTC              
BanI          121  GGTGCC               G^G[CT][GA]CC
             1010  GGTGCC              
BcnI         2381  CCGGG                CC^[GC]GG
             2756  CCGGG               
BsiHKAI       504  GAGCAC               G[AT]GC[AT]^C
             2006  GAGCTC              
BspLU11I      429  ACATGT               A^CATGT
             2697  ACATGT              
BsrDI         187  CATTGC               GCAATG(2/0)
             1161  GCAATG              
BssHII         95  GCGCGC               G^CGCGC
             1881  GCGCGC              
Eam1105I      670  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1984  GACTGAGGGTC         
EcoRI        2302  GAATTC               G^AATTC
             2436  GAATTC              
FauI          158  CCCGC                CCCGC(4/6)
             1905  CCCGC               
HindIII       614  AAGCTT               A^AGCTT
              777  AAGCTT              
KspI           20  CCGCGG               CCGC^GG
              117  CCGCGG              
MaeII        1809  ACGT                 A^CGT
             2859  ACGT                
NciI         2381  CCGGG                CC^[GC]GG
             2756  CCGGG               
NcoI          171  CCATGG               C^CATGG
              936  CCATGG              
PflMI        2290  CCAAAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2695  CCACATGTTGG         
PpuMI        1525  GGGTCCC              [GA]G^G[AT]CC[CT]
             2676  AGGTCCC             
SacII          20  CCGCGG               CCGC^GG
              117  CCGCGG              
TfiI          652  GATTC                G^A[AT]TC
             1066  GAATC               
Van91I       2290  CCAAAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2695  CCACATGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AvaII       
BalI        BanII       BbsI        BbvI        BcgI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII