##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AviII BalI
BbeI BfrI BglI BglII BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrGI BssSI
BstBI BstEII BstPI ClaI CpoI
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SfiI SfoI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 2263 CGCG CG^CG
AhdI 2919 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2901 GTGCAC G^TGCAC
ApaLI 2901 GTGCAC G^TGCAC
AspEI 2919 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2515 CCCGAG C^[CT]CG[GA]G
AvrII 1657 CCTAGG C^CTAGG
BanI 2444 GGCACC G^G[CT][GA]CC
BbsI 2916 GAAGAC GAAGAC(2/6)
BbvCI 1285 GCTGAGG CCTCAGC(-5/-2)
BciVI 1355 GGATAC GTATCC(6/5)
BlnI 1657 CCTAGG C^CTAGG
BlpI 106 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 106 GCTGAGC GC^T[ACGT]AGC
BpuAI 2916 GAAGAC GAAGAC(2/6)
BsoBI 2515 CCCGAG C^[CT]CG[GA]G
BspLU11I 3461 ACATGT A^CATGT
BspMI 814 GCAGGT ACCTGC(4/8)
BsrBI 1561 CCGCTC CCGCTC(-3/-3)
BsrDI 2315 GCAATG GCAATG(2/0)
BsrFI 2545 ACCGGC [GA]^CCGG[CT]
BssHII 2262 GCGCGC G^CGCGC
Bst1107I 136 GTATAC GTA^TAC
BstUI 2263 CGCG CG^CG
BstXI 668 CCAGGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 136 GTATAC GTA^TAC
CelII 106 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2545 ACCGGC [GA]^CCGG[CT]
DsaI 2009 CCATGG C^C[GA][CT]GG
Eam1105I 2919 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2369 GAATTC G^AATTC
HincII 2716 GTCGAC GT[CT]^[GA]AC
HindII 2716 GTCGAC GT[CT]^[GA]AC
MvnI 2263 CGCG CG^CG
NcoI 2009 CCATGG C^CATGG
NdeI 596 CATATG CA^TATG
NheI 529 GCTAGC G^CTAGC
PflMI 2289 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1524 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2716 GTCGAC G^TCGAC
SexAI 2057 ACCAGGT A^CC[AT]GGT
SgrAI 2544 CACCGGCG C[GA]^CCGG[CT]G
SspI 1986 AATATT AAT^ATT
Van91I 2289 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspI 1051 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3398 GACAAAGTC
BamHI 2285 GGATCC G^GATCC
2621 GGATCC
BbrPI 1948 CACGTG CAC^GTG
2562 CACGTG
BcgI 92 GCATGCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2685 GCACCTCACTCG
BclI 566 TGATCA T^GATCA
749 TGATCA
BsaAI 1948 CACGTG [CT]AC^GT[GA]
2562 CACGTG
BsaI 2050 GAGACC GGTCTC(1/5)
1687 GGTCTC
BsgI 1796 CTGCAC GTGCAG(16/14)
1717 GTGCAG
BstAPI 1589 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2987 GCAAGGAGTGC
DraI 1364 TTTAAA TTT^AAA
2151 TTTAAA
DraIII 2290 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2904 CACTGAGTG
FbaI 566 TGATCA T^GATCA
749 TGATCA
HgaI 1805 GACGC GACGC(5/10)
3183 GCGTC
PflFI 1051 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3398 GACAAAGTC
PmaCI 1948 CACGTG CAC^GTG
2562 CACGTG
PmlI 1948 CACGTG CAC^GTG
2562 CACGTG
SacI 1935 GAGCTC GAGCT^C
2518 GAGCTC
SapI 225 GAAGAGC GCTCTTC(1/4)
96 GCTCTTC
SmlI 481 CTCAAG C^T[CT][GA]AG
1089 CTTGAG
SphI 92 GCATGC GCATG^C
610 GCATGC
Tth111I 1051 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3398 GACAAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI