################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AviII BalI BbeI BfrI BglI BglII BsaBI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrGI BssSI BstBI BstEII BstPI ClaI CpoI DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SfuI SmaI SnaBI SpeI Sse8387I SspBI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 2263 CGCG CG^CG AhdI 2919 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2901 GTGCAC G^TGCAC ApaLI 2901 GTGCAC G^TGCAC AspEI 2919 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2515 CCCGAG C^[CT]CG[GA]G AvrII 1657 CCTAGG C^CTAGG BanI 2444 GGCACC G^G[CT][GA]CC BbsI 2916 GAAGAC GAAGAC(2/6) BbvCI 1285 GCTGAGG CCTCAGC(-5/-2) BciVI 1355 GGATAC GTATCC(6/5) BlnI 1657 CCTAGG C^CTAGG BlpI 106 GCTGAGC GC^T[ACGT]AGC Bpu1102I 106 GCTGAGC GC^T[ACGT]AGC BpuAI 2916 GAAGAC GAAGAC(2/6) BsoBI 2515 CCCGAG C^[CT]CG[GA]G BspLU11I 3461 ACATGT A^CATGT BspMI 814 GCAGGT ACCTGC(4/8) BsrBI 1561 CCGCTC CCGCTC(-3/-3) BsrDI 2315 GCAATG GCAATG(2/0) BsrFI 2545 ACCGGC [GA]^CCGG[CT] BssHII 2262 GCGCGC G^CGCGC Bst1107I 136 GTATAC GTA^TAC BstUI 2263 CGCG CG^CG BstXI 668 CCAGGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 136 GTATAC GTA^TAC CelII 106 GCTGAGC GC^T[ACGT]AGC Cfr10I 2545 ACCGGC [GA]^CCGG[CT] DsaI 2009 CCATGG C^C[GA][CT]GG Eam1105I 2919 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2369 GAATTC G^AATTC HincII 2716 GTCGAC GT[CT]^[GA]AC HindII 2716 GTCGAC GT[CT]^[GA]AC MvnI 2263 CGCG CG^CG NcoI 2009 CCATGG C^CATGG NdeI 596 CATATG CA^TATG NheI 529 GCTAGC G^CTAGC PflMI 2289 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1524 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 2716 GTCGAC G^TCGAC SexAI 2057 ACCAGGT A^CC[AT]GGT SgrAI 2544 CACCGGCG C[GA]^CCGG[CT]G SspI 1986 AATATT AAT^ATT Van91I 2289 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AspI 1051 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3398 GACAAAGTC BamHI 2285 GGATCC G^GATCC 2621 GGATCC BbrPI 1948 CACGTG CAC^GTG 2562 CACGTG BcgI 92 GCATGCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2685 GCACCTCACTCG BclI 566 TGATCA T^GATCA 749 TGATCA BsaAI 1948 CACGTG [CT]AC^GT[GA] 2562 CACGTG BsaI 2050 GAGACC GGTCTC(1/5) 1687 GGTCTC BsgI 1796 CTGCAC GTGCAG(16/14) 1717 GTGCAG BstAPI 1589 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2987 GCAAGGAGTGC DraI 1364 TTTAAA TTT^AAA 2151 TTTAAA DraIII 2290 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 2904 CACTGAGTG FbaI 566 TGATCA T^GATCA 749 TGATCA HgaI 1805 GACGC GACGC(5/10) 3183 GCGTC PflFI 1051 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3398 GACAAAGTC PmaCI 1948 CACGTG CAC^GTG 2562 CACGTG PmlI 1948 CACGTG CAC^GTG 2562 CACGTG SacI 1935 GAGCTC GAGCT^C 2518 GAGCTC SapI 225 GAAGAGC GCTCTTC(1/4) 96 GCTCTTC SmlI 481 CTCAAG C^T[CT][GA]AG 1089 CTTGAG SphI 92 GCATGC GCATG^C 610 GCATGC Tth111I 1051 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3398 GACAAAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI