##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AccI AclI AflII AgeI Aor51HI
ApaI AscI AseI AsnI Asp700I
AviII BbeI BbrPI BfrI BglII
BsaAI BsiEI BsiWI BsmI Bsp1407I
BspDI BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HincII HindII HpaI KasI
KspI MfeI MluI MseI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2095 GACGTC GACGT^C
Acc65I 3075 GGTACC G^GTACC
AcsI 1784 AAATTT [GA]^AATT[CT]
AcyI 2095 GACGTC G[GA]^CG[CT]C
AflIII 1248 ACATGT A^C[GA][CT]GT
Alw44I 2069 GTGCAC G^TGCAC
ApaLI 2069 GTGCAC G^TGCAC
ApoI 1784 AAATTT [GA]^AATT[CT]
Asp718I 3075 GGTACC G^GTACC
AspI 668 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 56 CCTAGG C^CTAGG
BalI 1089 TGGCCA TGG^CCA
BcgI 2332 CGACAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 426 GTATCC GTATCC(6/5)
BglI 2204 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 56 CCTAGG C^CTAGG
BlpI 3116 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3116 GCTCAGC GC^T[ACGT]AGC
BsaBI 610 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2095 GACGTC G[GA]^CG[CT]C
BsgI 2861 GTGCAG GTGCAG(16/14)
BspHI 2568 TCATGA T^CATGA
BspLU11I 1248 ACATGT A^CATGT
BspMI 1752 ACCTGC ACCTGC(4/8)
BsrFI 506 ACCGGC [GA]^CCGG[CT]
BssSI 776 CACGAG CACGAG(-5/-1)
BstAPI 1137 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3116 GCTCAGC GC^T[ACGT]AGC
Cfr10I 506 ACCGGC [GA]^CCGG[CT]
Hin1I 2095 GACGTC G[GA]^CG[CT]C
HindIII 2168 AAGCTT A^AGCTT
KpnI 3075 GGTACC GGTAC^C
MamI 610 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1089 TGGCCA TGG^CCA
MscI 1089 TGGCCA TGG^CCA
NheI 484 GCTAGC G^CTAGC
PflFI 668 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 59 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2568 TCATGA T^CATGA
ScaI 108 AGTACT AGT^ACT
SexAI 645 ACCTGGT A^CC[AT]GGT
SmaI 2989 CCCGGG CCC^GGG
Tth111I 668 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2989 CCCGGG C^CCGGG
XmaI 2989 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 687 TCCGGA T^CCGGA
3009 TCCGGA
AhdI 1313 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2090 GACATGACGTC
AlwNI 2214 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
2657 CAGGCTCTG
AocI 2119 CCTCAGG CC^T[ACGT]AGG
3206 CCTGAGG
AspEI 1313 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2090 GACATGACGTC
AvaI 1773 CTCGGG C^[CT]CG[GA]G
2989 CCCGGG
BamHI 32 GGATCC G^GATCC
2730 GGATCC
BclI 960 TGATCA T^GATCA
1914 TGATCA
BseAI 687 TCCGGA T^CCGGA
3009 TCCGGA
BsoBI 1773 CTCGGG C^[CT]CG[GA]G
2989 CCCGGG
BspEI 687 TCCGGA T^CCGGA
3009 TCCGGA
Bsu36I 2119 CCTCAGG CC^T[ACGT]AGG
3206 CCTGAGG
DraII 59 AGGTCCC [GA]G^G[ACGT]CC[CT]
261 AGGGCCT
DrdI 1333 GACAGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3183 GACTGGGGAGTC
EaeI 432 TGGCCG [CT]^GGCC[GA]
1089 TGGCCA
Eam1105I 1313 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2090 GACATGACGTC
Eco81I 2119 CCTCAGG CC^T[ACGT]AGG
3206 CCTGAGG
EcoNI 646 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1639 CCTGGTACAGG
EcoO109I 59 AGGTCCC [GA]G^G[ACGT]CC[CT]
261 AGGGCCT
FbaI 960 TGATCA T^GATCA
1914 TGATCA
MroI 687 TCCGGA T^CCGGA
3009 TCCGGA
NspI 1248 ACATGT [GA]CATG^[CT]
2825 ACATGC
PflMI 1582 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2656 CCAGGCTCTGG
SfcI 492 CTGTAG C^T[GA][CT]AG
1021 CTACAG
SmlI 1448 CTCAAG C^T[CT][GA]AG
3243 CTTGAG
Tsp509I 464 AATT ^AATT
1785 AATT
Van91I 1582 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2656 CCAGGCTCTGG
XcmI 2656 CCAGGCTCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2757 CCACCAACGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII