##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BcgI BclI
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI Eam1105I
EcoO65I EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 1020 ACATGT A^C[GA][CT]GT
Aor51HI 212 AGCGCT AGC^GCT
AvaI 660 CTCGAG C^[CT]CG[GA]G
BamHI 941 GGATCC G^GATCC
BbeI 200 GGCGCC GGCGC^C
BbrPI 2180 CACGTG CAC^GTG
BbvCI 41 GCTGAGG CCTCAGC(-5/-2)
BciVI 823 GGATAC GTATCC(6/5)
BglI 1733 GCCTCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 727 AGATCT A^GATCT
BsaAI 2180 CACGTG [CT]AC^GT[GA]
BsoBI 660 CTCGAG C^[CT]CG[GA]G
BspLU11I 1020 ACATGT A^CATGT
BspMI 744 ACCTGC ACCTGC(4/8)
BsrDI 2500 CATTGC GCAATG(2/0)
BstAPI 1757 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1959 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1433 GACCAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 212 AGCGCT AGC^GCT
EcoRV 2470 GATATC GAT^ATC
KasI 200 GGCGCC G^GCGCC
NarI 200 GGCGCC GG^CGCC
PaeR7I 660 CTCGAG C^TCGAG
PmaCI 2180 CACGTG CAC^GTG
PmlI 2180 CACGTG CAC^GTG
SbfI 2761 CCTGCAGG CCTGCA^GG
ScaI 696 AGTACT AGT^ACT
SfoI 200 GGCGCC GGC^GCC
Sse8387I 2761 CCTGCAGG CCTGCA^GG
XhoI 660 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 200 GGCGCC G[GA]^CG[CT]C
2324 GACGCC
AflII 371 CTTAAG C^TTAAG
1046 CTTAAG
AlwNI 488 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1758 CAGTCTCTG
AocI 890 CCTGAGG CC^T[ACGT]AGG
1511 CCTGAGG
Asp700I 490 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2705 GAAGTGGTTC
AspHI 1297 GAGCTC G[AT]GC[AT]^C
2275 GAGCTC
BalI 1678 TGGCCA TGG^CCA
2059 TGGCCA
BanI 200 GGCGCC G^G[CT][GA]CC
1858 GGTGCC
BfrI 371 CTTAAG C^TTAAG
1046 CTTAAG
BsaHI 200 GGCGCC G[GA]^CG[CT]C
2324 GACGCC
BsiHKAI 1297 GAGCTC G[AT]GC[AT]^C
2275 GAGCTC
BsmBI 1399 CGTCTC CGTCTC(1/5)
2165 GAGACG
BsrBI 98 GAGCGG CCGCTC(-3/-3)
2354 GAGCGG
BstXI 1721 CCAGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2686 CCATGTTTGTGG
Bsu36I 890 CCTGAGG CC^T[ACGT]AGG
1511 CCTGAGG
EarI 1223 GAAGAG CTCTTC(1/4)
1517 GAAGAG
Eco81I 890 CCTGAGG CC^T[ACGT]AGG
1511 CCTGAGG
EcoNI 1675 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2761 CCTGCAGGAGG
EcoRI 800 GAATTC G^AATTC
1462 GAATTC
HaeII 200 GGCGCC [GA]GCGC^[CT]
212 AGCGCT
Hin1I 200 GGCGCC G[GA]^CG[CT]C
2324 GACGCC
HindIII 170 AAGCTT A^AGCTT
2528 AAGCTT
Ksp632I 1223 GAAGAG CTCTTC(1/4)
1517 GAAGAG
MaeII 1119 ACGT A^CGT
2181 ACGT
MluNI 1678 TGGCCA TGG^CCA
2059 TGGCCA
MscI 1678 TGGCCA TGG^CCA
2059 TGGCCA
MslI 2479 CACTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2687 CATGTTTGTG
NaeI 71 GCCGGC GCC^GGC
2073 GCCGGC
NcoI 634 CCATGG C^CATGG
2484 CCATGG
NgoMIV 71 GCCGGC G^CCGGC
2073 GCCGGC
PpuMI 1176 AGGACCC [GA]G^G[AT]CC[CT]
1724 GGGTCCT
PstI 2615 CTGCAG CTGCA^G
2762 CTGCAG
SacI 1297 GAGCTC GAGCT^C
2275 GAGCTC
StuI 1683 AGGCCT AGG^CCT
1821 AGGCCT
XcmI 399 CCAAGGTGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2271 CCACGAGCTCTATGG
XmnI 490 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2705 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII