##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccI AccII AccIII AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AviII BamHI
BbeI BciVI BfrI BglII BsaBI
BseAI BsiWI BsmI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstBI
BstUI BstZ17I Bsu36I ClaI CpoI
DraIII Eco81I EcoNI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AclI 1541 AACGTT AA^CGTT
AcsI 920 GAATTC [GA]^AATT[CT]
AhdI 2233 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 856 AGCGCT AGC^GCT
ApaI 897 GGGCCC GGGCC^C
ApoI 920 GAATTC [GA]^AATT[CT]
Asp700I 854 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2233 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2121 GTGCTC G[AT]GC[AT]^C
AspI 1291 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2630 CCTAGG C^CTAGG
BalI 2171 TGGCCA TGG^CCA
BcgI 1668 GCACATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2630 CCTAGG C^CTAGG
BlpI 24 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC
BsaWI 428 ACCGGA [AT]^CCGG[AT]
BsiEI 1140 CGGCCG CG[GA][CT]^CG
BsiHKAI 2121 GTGCTC G[AT]GC[AT]^C
Bsp1407I 2010 TGTACA T^GTACA
BspLU11I 1005 ACATGT A^CATGT
BsrFI 1756 GCCGGC [GA]^CCGG[CT]
BsrGI 2010 TGTACA T^GTACA
BssSI 1676 CTCGTG CACGAG(-5/-1)
BstAPI 1162 GCACGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1420 GGTGACC G^GT[ACGT]ACC
BstPI 1420 GGTGACC G^GT[ACGT]ACC
BstXI 227 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 24 GCTGAGC GC^T[ACGT]AGC
CfoI 857 GCGC GCG^C
Cfr10I 1756 GCCGGC [GA]^CCGG[CT]
EagI 1140 CGGCCG C^GGCCG
Eam1105I 2233 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2477 GAAGAG CTCTTC(1/4)
EclXI 1140 CGGCCG C^GGCCG
Eco47III 856 AGCGCT AGC^GCT
Eco52I 1140 CGGCCG C^GGCCG
EcoO65I 1420 GGTGACC G^GT[ACGT]ACC
EcoRI 920 GAATTC G^AATTC
HaeII 856 AGCGCT [GA]GCGC^[CT]
HhaI 857 GCGC GCG^C
HinP1I 857 GCGC G^CGC
Ksp632I 2477 GAAGAG CTCTTC(1/4)
MluNI 2171 TGGCCA TGG^CCA
MscI 2171 TGGCCA TGG^CCA
NaeI 1756 GCCGGC GCC^GGC
NgoMIV 1756 GCCGGC G^CCGGC
NheI 806 GCTAGC G^CTAGC
PflFI 1291 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 77 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1901 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1541 AACGTT AA^CGTT
SmlI 2156 CTCAAG C^T[CT][GA]AG
SphI 1158 GCATGC GCATG^C
SspBI 2010 TGTACA T^GTACA
StuI 452 AGGCCT AGG^CCT
SwaI 558 ATTTAAAT ATTT^AAAT
Tth111I 1291 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 77 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 854 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 1181 GACGTC GACGT^C
1478 GACGTC
BanI 1089 GGCACC G^G[CT][GA]CC
1526 GGCACC
BbrPI 1163 CACGTG CAC^GTG
1501 CACGTG
BbvCI 662 CCTCAGC CCTCAGC(-5/-2)
2596 CCTCAGC
BclI 1461 TGATCA T^GATCA
1638 TGATCA
BglI 699 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1378 GCCCCCTCGGC
BsaI 1745 GAGACC GGTCTC(1/5)
2338 GAGACC
BsgI 1231 CTGCAC GTGCAG(16/14)
1248 GTGCAG
BstYI 333 GGATCT [GA]^GATC[CT]
2020 AGATCC
DrdI 1319 GACAGAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1472 GACTCGGACGTC
EaeI 1140 CGGCCG [CT]^GGCC[GA]
2171 TGGCCA
FbaI 1461 TGATCA T^GATCA
1638 TGATCA
HindIII 2362 AAGCTT A^AGCTT
2580 AAGCTT
MflI 333 GGATCT [GA]^GATC[CT]
2020 AGATCC
NspI 1005 ACATGT [GA]CATG^[CT]
1158 GCATGC
PmaCI 1163 CACGTG CAC^GTG
1501 CACGTG
PmlI 1163 CACGTG CAC^GTG
1501 CACGTG
Tsp509I 414 AATT ^AATT
921 AATT
XhoII 333 GGATCT [GA]^GATC[CT]
2020 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I XcmI