################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## Acc65I AccI AccII AccIII AflII AgeI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AviII BamHI BbeI BciVI BfrI BglII BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I ClaI CpoI DraIII Eco81I EcoNI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AclI 1541 AACGTT AA^CGTT AcsI 920 GAATTC [GA]^AATT[CT] AhdI 2233 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 856 AGCGCT AGC^GCT ApaI 897 GGGCCC GGGCC^C ApoI 920 GAATTC [GA]^AATT[CT] Asp700I 854 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2233 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2121 GTGCTC G[AT]GC[AT]^C AspI 1291 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2630 CCTAGG C^CTAGG BalI 2171 TGGCCA TGG^CCA BcgI 1668 GCACATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 2630 CCTAGG C^CTAGG BlpI 24 GCTGAGC GC^T[ACGT]AGC Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC BsaWI 428 ACCGGA [AT]^CCGG[AT] BsiEI 1140 CGGCCG CG[GA][CT]^CG BsiHKAI 2121 GTGCTC G[AT]GC[AT]^C Bsp1407I 2010 TGTACA T^GTACA BspLU11I 1005 ACATGT A^CATGT BsrFI 1756 GCCGGC [GA]^CCGG[CT] BsrGI 2010 TGTACA T^GTACA BssSI 1676 CTCGTG CACGAG(-5/-1) BstAPI 1162 GCACGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1420 GGTGACC G^GT[ACGT]ACC BstPI 1420 GGTGACC G^GT[ACGT]ACC BstXI 227 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 24 GCTGAGC GC^T[ACGT]AGC CfoI 857 GCGC GCG^C Cfr10I 1756 GCCGGC [GA]^CCGG[CT] EagI 1140 CGGCCG C^GGCCG Eam1105I 2233 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2477 GAAGAG CTCTTC(1/4) EclXI 1140 CGGCCG C^GGCCG Eco47III 856 AGCGCT AGC^GCT Eco52I 1140 CGGCCG C^GGCCG EcoO65I 1420 GGTGACC G^GT[ACGT]ACC EcoRI 920 GAATTC G^AATTC HaeII 856 AGCGCT [GA]GCGC^[CT] HhaI 857 GCGC GCG^C HinP1I 857 GCGC G^CGC Ksp632I 2477 GAAGAG CTCTTC(1/4) MluNI 2171 TGGCCA TGG^CCA MscI 2171 TGGCCA TGG^CCA NaeI 1756 GCCGGC GCC^GGC NgoMIV 1756 GCCGGC G^CCGGC NheI 806 GCTAGC G^CTAGC PflFI 1291 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 77 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1901 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1541 AACGTT AA^CGTT SmlI 2156 CTCAAG C^T[CT][GA]AG SphI 1158 GCATGC GCATG^C SspBI 2010 TGTACA T^GTACA StuI 452 AGGCCT AGG^CCT SwaI 558 ATTTAAAT ATTT^AAAT Tth111I 1291 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 77 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 854 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AatII 1181 GACGTC GACGT^C 1478 GACGTC BanI 1089 GGCACC G^G[CT][GA]CC 1526 GGCACC BbrPI 1163 CACGTG CAC^GTG 1501 CACGTG BbvCI 662 CCTCAGC CCTCAGC(-5/-2) 2596 CCTCAGC BclI 1461 TGATCA T^GATCA 1638 TGATCA BglI 699 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1378 GCCCCCTCGGC BsaI 1745 GAGACC GGTCTC(1/5) 2338 GAGACC BsgI 1231 CTGCAC GTGCAG(16/14) 1248 GTGCAG BstYI 333 GGATCT [GA]^GATC[CT] 2020 AGATCC DrdI 1319 GACAGAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1472 GACTCGGACGTC EaeI 1140 CGGCCG [CT]^GGCC[GA] 2171 TGGCCA FbaI 1461 TGATCA T^GATCA 1638 TGATCA HindIII 2362 AAGCTT A^AGCTT 2580 AAGCTT MflI 333 GGATCT [GA]^GATC[CT] 2020 AGATCC NspI 1005 ACATGT [GA]CATG^[CT] 1158 GCATGC PmaCI 1163 CACGTG CAC^GTG 1501 CACGTG PmlI 1163 CACGTG CAC^GTG 1501 CACGTG Tsp509I 414 AATT ^AATT 921 AATT XhoII 333 GGATCT [GA]^GATC[CT] 2020 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AlwNI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I XcmI