##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BfrI BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI NarI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 116 GTAGAC GT^[AC][GT]AC
AccII 593 CGCG CG^CG
AclI 1150 AACGTT AA^CGTT
AflIII 970 ACGTGT A^C[GA][CT]GT
ApaI 1016 GGGCCC GGGCC^C
AspHI 1475 GTGCTC G[AT]GC[AT]^C
AvaI 1900 CCCGAG C^[CT]CG[GA]G
BanII 1016 GGGCCC G[GA]GC[CT]^C
BbvCI 3213 CCTCAGC CCTCAGC(-5/-2)
BglI 1356 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1978 AGATCT A^GATCT
BsaAI 969 TACGTG [CT]AC^GT[GA]
BsaWI 1067 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1475 GTGCTC G[AT]GC[AT]^C
BsmBI 923 CGTCTC CGTCTC(1/5)
BsmI 1032 GCATTC GAATGC(1/-1)
BsoBI 1900 CCCGAG C^[CT]CG[GA]G
BspHI 2383 TCATGA T^CATGA
BsrFI 1649 GCCGGC [GA]^CCGG[CT]
BstUI 593 CGCG CG^CG
Cfr10I 1649 GCCGGC [GA]^CCGG[CT]
DsaI 1290 CCACGG C^C[GA][CT]GG
EcoNI 254 CCTAAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1716 GAATTC G^AATTC
MvnI 593 CGCG CG^CG
NaeI 1649 GCCGGC GCC^GGC
NdeI 886 CATATG CA^TATG
NgoMIV 1649 GCCGGC G^CCGGC
Psp1406I 1150 AACGTT AA^CGTT
RcaI 2383 TCATGA T^CATGA
SphI 438 GCATGC GCATG^C
SspI 1686 AATATT AAT^ATT
TaqI 1546 TCGA T^CGA
TthHB8I 1546 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 396 CCTCAGG CC^T[ACGT]AGG
1099 CCTCAGG
Aor51HI 3064 AGCGCT AGC^GCT
3157 AGCGCT
BcgI 623 GCACAGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1368 GCAGGATGTTCG
BsrDI 1849 CATTGC GCAATG(2/0)
2521 GCAATG
BssSI 1704 CACGAG CACGAG(-5/-1)
1768 CACGAG
BstYI 1978 AGATCT [GA]^GATC[CT]
3075 AGATCC
Bsu36I 396 CCTCAGG CC^T[ACGT]AGG
1099 CCTCAGG
Eco47III 3064 AGCGCT AGC^GCT
3157 AGCGCT
Eco81I 396 CCTCAGG CC^T[ACGT]AGG
1099 CCTCAGG
FauI 2093 CCCGC CCCGC(4/6)
1892 GCGGG
MaeII 970 ACGT A^CGT
1151 ACGT
MfeI 17 CAATTG C^AATTG
2405 CAATTG
MflI 1978 AGATCT [GA]^GATC[CT]
3075 AGATCC
MunI 17 CAATTG C^AATTG
2405 CAATTG
NspI 438 GCATGC [GA]CATG^[CT]
2320 ACATGC
PpuMI 425 GGGTCCC [GA]G^G[AT]CC[CT]
3143 AGGACCC
SbfI 2106 CCTGCAGG CCTGCA^GG
2475 CCTGCAGG
Sse8387I 2106 CCTGCAGG CCTGCA^GG
2475 CCTGCAGG
XhoII 1978 AGATCT [GA]^GATC[CT]
3075 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI AspEI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI Eam1105I
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI