##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AvaI
AvrII BamHI BanI BbeI BbrPI
BcgI BclI BlnI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspMI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FbaI FseI HgaI Hin1I HpaI
KasI KpnI MamI MfeI MroI
MunI NarI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 1254 CTTAAG C^TTAAG
AgeI 1079 ACCGGT A^CCGGT
AspHI 1850 GAGCAC G[AT]GC[AT]^C
AspI 915 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 458 TGGCCA TGG^CCA
BciVI 53 GGATAC GTATCC(6/5)
BfrI 1254 CTTAAG C^TTAAG
BsaI 1016 GGTCTC GGTCTC(1/5)
BsaWI 1079 ACCGGT [AT]^CCGG[AT]
BsgI 1959 CTGCAC GTGCAG(16/14)
BsiHKAI 1850 GAGCAC G[AT]GC[AT]^C
BsmBI 920 CGTCTC CGTCTC(1/5)
BspLU11I 2778 ACATGT A^CATGT
BsrBI 829 CCGCTC CCGCTC(-3/-3)
BssHII 314 GCGCGC G^CGCGC
BssSI 1962 CACGAG CACGAG(-5/-1)
DraIII 2470 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2 CCGCGG C^C[GA][CT]GG
EcoT14I 1657 CCTTGG C^C[AT][AT]GG
HindIII 2371 AAGCTT A^AGCTT
KspI 2 CCGCGG CCGC^GG
MluI 2041 ACGCGT A^CGCGT
MluNI 458 TGGCCA TGG^CCA
MscI 458 TGGCCA TGG^CCA
NaeI 465 GCCGGC GCC^GGC
NgoMIV 465 GCCGGC G^CCGGC
PflFI 915 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 197 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1079 ACCGGT A^CCGGT
PshAI 1038 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1307 CTGCAG CTGCA^G
SacII 2 CCGCGG CCGC^GG
SapI 1980 GCTCTTC GCTCTTC(1/4)
ScaI 1663 AGTACT AGT^ACT
SpeI 2100 ACTAGT A^CTAGT
SspI 2790 AATATT AAT^ATT
StyI 1657 CCTTGG C^C[AT][AT]GG
Tth111I 915 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 197 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 2041 ACGCGT A^C[GA][CT]GT
2778 ACATGT
AhdI 779 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1110 GACTTCGTGTC
AspEI 779 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1110 GACTTCGTGTC
AviII 1330 TGCGCA TGC^GCA
2315 TGCGCA
BbsI 1404 GAAGAC GAAGAC(2/6)
2607 GAAGAC
BcnI 35 CCGGG CC^[GC]GG
2397 CCGGG
BglI 460 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1708 GCCAGCGAGGC
BglII 2127 AGATCT A^GATCT
2673 AGATCT
BlpI 401 GCTGAGC GC^T[ACGT]AGC
1364 GCTGAGC
Bpu1102I 401 GCTGAGC GC^T[ACGT]AGC
1364 GCTGAGC
BpuAI 1404 GAAGAC GAAGAC(2/6)
2607 GAAGAC
BseRI 1275 CTCCTC GAGGAG(10/8)
2187 GAGGAG
Bsp1407I 1201 TGTACA T^GTACA
1834 TGTACA
BspHI 1678 TCATGA T^CATGA
2221 TCATGA
BsrDI 766 CATTGC GCAATG(2/0)
1717 GCAATG
BsrFI 465 GCCGGC [GA]^CCGG[CT]
1079 ACCGGT
BsrGI 1201 TGTACA T^GTACA
1834 TGTACA
BstAPI 89 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
324 GCAGAGTGTGC
CelII 401 GCTGAGC GC^T[ACGT]AGC
1364 GCTGAGC
Cfr10I 465 GCCGGC [GA]^CCGG[CT]
1079 ACCGGT
DrdI 764 GACATTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1967 GACACCCCTGTC
EaeI 458 TGGCCA [CT]^GGCC[GA]
1105 TGGCCG
Eam1105I 779 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1110 GACTTCGTGTC
EcoRV 516 GATATC GAT^ATC
1293 GATATC
FspI 1330 TGCGCA TGC^GCA
2315 TGCGCA
HincII 482 GTCAAC GT[CT]^[GA]AC
1897 GTCAAC
HindII 482 GTCAAC GT[CT]^[GA]AC
1897 GTCAAC
NciI 35 CCGGG CC^[GC]GG
2397 CCGGG
NheI 1469 GCTAGC G^CTAGC
2595 GCTAGC
PpuMI 734 GGGACCC [GA]G^G[AT]CC[CT]
800 AGGACCT
RcaI 1678 TCATGA T^CATGA
2221 TCATGA
SspBI 1201 TGTACA T^GTACA
1834 TGTACA
StuI 1181 AGGCCT AGG^CCT
2341 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AvaII BanII BbvCI BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI