################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccII AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AvaI AviII BbrPI BcgI BclI BfrI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI BstBI BstUI ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KspI MamI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 360 GGTACC G^GTACC AccI 652 GTATAC GT^[AC][GT]AC AhdI 457 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 56 CCTTAGG CC^T[ACGT]AGG ApaI 1771 GGGCCC GGGCC^C Asp718I 360 GGTACC G^GTACC AspEI 457 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1730 CCTAGG C^CTAGG BbeI 1592 GGCGCC GGCGC^C BciVI 2668 GTATCC GTATCC(6/5) BglI 2391 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2018 AGATCT A^GATCT BlnI 1730 CCTAGG C^CTAGG BlpI 1530 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1530 GCTCAGC GC^T[ACGT]AGC BsaI 294 GAGACC GGTCTC(1/5) BsaWI 354 TCCGGT [AT]^CCGG[AT] BspLU11I 2739 ACATGT A^CATGT BspMI 297 ACCTGC ACCTGC(4/8) BsrBI 1622 CCGCTC CCGCTC(-3/-3) BsrDI 1220 CATTGC GCAATG(2/0) Bst1107I 652 GTATAC GTA^TAC BstEII 1041 GGTGACC G^GT[ACGT]ACC BstPI 1041 GGTGACC G^GT[ACGT]ACC BstZ17I 652 GTATAC GTA^TAC Bsu36I 56 CCTTAGG CC^T[ACGT]AGG CelII 1530 GCTCAGC GC^T[ACGT]AGC DrdI 1794 GACAGAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 457 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 56 CCTTAGG CC^T[ACGT]AGG EcoO65I 1041 GGTGACC G^GT[ACGT]ACC KasI 1592 GGCGCC G^GCGCC KpnI 360 GGTACC GGTAC^C MaeII 1653 ACGT A^CGT NarI 1592 GGCGCC GG^CGCC PflMI 88 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfiI 2390 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1592 GGCGCC GGC^GCC StuI 1320 AGGCCT AGG^CCT Tsp509I 2466 AATT ^AATT Van91I 88 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 1592 GGCGCC G[GA]^CG[CT]C 1711 GACGCC AflIII 1653 ACGTGT A^C[GA][CT]GT 2739 ACATGT AspI 148 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1268 GACTATGTC BamHI 36 GGATCC G^GATCC 687 GGATCC BbsI 2108 GAAGAC GAAGAC(2/6) 2432 GTCTTC BbvCI 47 CCTCAGC CCTCAGC(-5/-2) 1193 CCTCAGC BcnI 822 CCGGG CC^[GC]GG 2124 CCCGG BpuAI 2108 GAAGAC GAAGAC(2/6) 2432 GTCTTC BsaHI 1592 GGCGCC G[GA]^CG[CT]C 1711 GACGCC DraIII 963 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 2169 CACCTTGTG FauI 1208 CCCGC CCCGC(4/6) 1621 CCCGC HgaI 1711 GACGC GACGC(5/10) 2535 GACGC Hin1I 1592 GGCGCC G[GA]^CG[CT]C 1711 GACGCC HincII 2147 GTTGAC GT[CT]^[GA]AC 2445 GTTGAC HindII 2147 GTTGAC GT[CT]^[GA]AC 2445 GTTGAC HindIII 344 AAGCTT A^AGCTT 2404 AAGCTT NciI 822 CCGGG CC^[GC]GG 2124 CCCGG NcoI 708 CCATGG C^CATGG 1128 CCATGG NspI 1016 GCATGT [GA]CATG^[CT] 2739 ACATGT PflFI 148 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1268 GACTATGTC SacI 912 GAGCTC GAGCT^C 2128 GAGCTC SmlI 779 CTCAAG C^T[CT][GA]AG 1973 CTCAAG TaqI 735 TCGA T^CGA 1557 TCGA Tth111I 148 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1268 GACTATGTC TthHB8I 735 TCGA T^CGA 1557 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I TspRI XhoII