##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AvaI AviII BbrPI BcgI BclI
BfrI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI BstBI BstUI ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 360 GGTACC G^GTACC
AccI 652 GTATAC GT^[AC][GT]AC
AhdI 457 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 56 CCTTAGG CC^T[ACGT]AGG
ApaI 1771 GGGCCC GGGCC^C
Asp718I 360 GGTACC G^GTACC
AspEI 457 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1730 CCTAGG C^CTAGG
BbeI 1592 GGCGCC GGCGC^C
BciVI 2668 GTATCC GTATCC(6/5)
BglI 2391 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2018 AGATCT A^GATCT
BlnI 1730 CCTAGG C^CTAGG
BlpI 1530 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1530 GCTCAGC GC^T[ACGT]AGC
BsaI 294 GAGACC GGTCTC(1/5)
BsaWI 354 TCCGGT [AT]^CCGG[AT]
BspLU11I 2739 ACATGT A^CATGT
BspMI 297 ACCTGC ACCTGC(4/8)
BsrBI 1622 CCGCTC CCGCTC(-3/-3)
BsrDI 1220 CATTGC GCAATG(2/0)
Bst1107I 652 GTATAC GTA^TAC
BstEII 1041 GGTGACC G^GT[ACGT]ACC
BstPI 1041 GGTGACC G^GT[ACGT]ACC
BstZ17I 652 GTATAC GTA^TAC
Bsu36I 56 CCTTAGG CC^T[ACGT]AGG
CelII 1530 GCTCAGC GC^T[ACGT]AGC
DrdI 1794 GACAGAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 457 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 56 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1041 GGTGACC G^GT[ACGT]ACC
KasI 1592 GGCGCC G^GCGCC
KpnI 360 GGTACC GGTAC^C
MaeII 1653 ACGT A^CGT
NarI 1592 GGCGCC GG^CGCC
PflMI 88 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfiI 2390 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1592 GGCGCC GGC^GCC
StuI 1320 AGGCCT AGG^CCT
Tsp509I 2466 AATT ^AATT
Van91I 88 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1592 GGCGCC G[GA]^CG[CT]C
1711 GACGCC
AflIII 1653 ACGTGT A^C[GA][CT]GT
2739 ACATGT
AspI 148 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1268 GACTATGTC
BamHI 36 GGATCC G^GATCC
687 GGATCC
BbsI 2108 GAAGAC GAAGAC(2/6)
2432 GTCTTC
BbvCI 47 CCTCAGC CCTCAGC(-5/-2)
1193 CCTCAGC
BcnI 822 CCGGG CC^[GC]GG
2124 CCCGG
BpuAI 2108 GAAGAC GAAGAC(2/6)
2432 GTCTTC
BsaHI 1592 GGCGCC G[GA]^CG[CT]C
1711 GACGCC
DraIII 963 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2169 CACCTTGTG
FauI 1208 CCCGC CCCGC(4/6)
1621 CCCGC
HgaI 1711 GACGC GACGC(5/10)
2535 GACGC
Hin1I 1592 GGCGCC G[GA]^CG[CT]C
1711 GACGCC
HincII 2147 GTTGAC GT[CT]^[GA]AC
2445 GTTGAC
HindII 2147 GTTGAC GT[CT]^[GA]AC
2445 GTTGAC
HindIII 344 AAGCTT A^AGCTT
2404 AAGCTT
NciI 822 CCGGG CC^[GC]GG
2124 CCCGG
NcoI 708 CCATGG C^CATGG
1128 CCATGG
NspI 1016 GCATGT [GA]CATG^[CT]
2739 ACATGT
PflFI 148 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1268 GACTATGTC
SacI 912 GAGCTC GAGCT^C
2128 GAGCTC
SmlI 779 CTCAAG C^T[CT][GA]AG
1973 CTCAAG
TaqI 735 TCGA T^CGA
1557 TCGA
Tth111I 148 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1268 GACTATGTC
TthHB8I 735 TCGA T^CGA
1557 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I TspRI
XhoII