##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I AspEI AspI
AviII BbrPI BciVI BclI BglII
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfuI SmaI SnaBI SpeI SspBI
SspI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflII 1654 CTTAAG C^TTAAG
AvrII 3477 CCTAGG C^CTAGG
BalI 2964 TGGCCA TGG^CCA
BbsI 3270 GTCTTC GAAGAC(2/6)
BcgI 1841 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1654 CTTAAG C^TTAAG
BlnI 3477 CCTAGG C^CTAGG
BpuAI 3270 GTCTTC GAAGAC(2/6)
BsaWI 1964 ACCGGA [AT]^CCGG[AT]
BsmBI 2502 CGTCTC CGTCTC(1/5)
BssHII 1691 GCGCGC G^CGCGC
BssSI 2816 CTCGTG CACGAG(-5/-1)
BstEII 367 GGTGACC G^GT[ACGT]ACC
BstPI 367 GGTGACC G^GT[ACGT]ACC
DraI 3375 TTTAAA TTT^AAA
DraIII 1888 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3261 GACACCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 367 GGTGACC G^GT[ACGT]ACC
EcoRV 209 GATATC GAT^ATC
MaeII 2459 ACGT A^CGT
MluNI 2964 TGGCCA TGG^CCA
MscI 2964 TGGCCA TGG^CCA
NaeI 1828 GCCGGC GCC^GGC
NcoI 149 CCATGG C^CATGG
NgoMIV 1828 GCCGGC G^CCGGC
NheI 2568 GCTAGC G^CTAGC
PaeR7I 1696 CTCGAG C^TCGAG
SbfI 2370 CCTGCAGG CCTGCA^GG
SgrAI 1827 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 2370 CCTGCAGG CCTGCA^GG
XhoI 1696 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Acc65I 2259 GGTACC G^GTACC
3354 GGTACC
AcsI 2287 GAATTT [GA]^AATT[CT]
3306 GAATTT
Alw44I 896 GTGCAC G^TGCAC
1585 GTGCAC
ApaLI 896 GTGCAC G^TGCAC
1585 GTGCAC
ApoI 2287 GAATTT [GA]^AATT[CT]
3306 GAATTT
Asp718I 2259 GGTACC G^GTACC
3354 GGTACC
BbvCI 1735 GCTGAGG CCTCAGC(-5/-2)
3016 GCTGAGG
BsmI 1980 GAATGC GAATGC(1/-1)
378 GCATTC
BsrFI 711 ACCGGC [GA]^CCGG[CT]
1828 GCCGGC
BstAPI 1208 GCAGGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2840 GCATCATGTGC
Cfr10I 711 ACCGGC [GA]^CCGG[CT]
1828 GCCGGC
FauI 1229 GCGGG CCCGC(4/6)
1866 GCGGG
KpnI 2259 GGTACC GGTAC^C
3354 GGTACC
SacI 2656 GAGCTC GAGCT^C
2740 GAGCTC
SapI 2136 GAAGAGC GCTCTTC(1/4)
3487 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BamHI BanI BanII BbeI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII