##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AspI BbeI BcgI
BclI BfrI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SspBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AocI 238 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1350 AGCGCT AGC^GCT
Asp700I 265 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 2893 GGACC G^G[AT]CC
AviII 707 TGCGCA TGC^GCA
BbrPI 586 CACGTG CAC^GTG
BglII 906 AGATCT A^GATCT
BsaAI 586 CACGTG [CT]AC^GT[GA]
BsaBI 1356 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 1257 GCAGGT ACCTGC(4/8)
BsrBI 757 GAGCGG CCGCTC(-3/-3)
BsrFI 1621 GCCGGT [GA]^CCGG[CT]
BstEII 1404 GGTCACC G^GT[ACGT]ACC
BstPI 1404 GGTCACC G^GT[ACGT]ACC
Bsu36I 238 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1621 GCCGGT [GA]^CCGG[CT]
DraI 3166 TTTAAA TTT^AAA
DrdI 3053 GACGCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1350 AGCGCT AGC^GCT
Eco81I 238 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1404 GGTCACC G^GT[ACGT]ACC
FspI 707 TGCGCA TGC^GCA
HincII 1762 GTCAAC GT[CT]^[GA]AC
HindII 1762 GTCAAC GT[CT]^[GA]AC
MamI 1356 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1710 GCTAGC G^CTAGC
PaeR7I 2078 CTCGAG C^TCGAG
PmaCI 586 CACGTG CAC^GTG
PmlI 586 CACGTG CAC^GTG
PpuMI 2892 GGGACCC [GA]G^G[AT]CC[CT]
SmaI 1903 CCCGGG CCC^GGG
SpeI 2818 ACTAGT A^CTAGT
Tsp509I 1814 AATT ^AATT
XcmI 1706 CCACGCTAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2078 CTCGAG C^TCGAG
XmaCI 1903 CCCGGG C^CCGGG
XmaI 1903 CCCGGG C^CCGGG
XmnI 265 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 284 GTGCAC G^TGCAC
1856 GTGCAC
ApaLI 284 GTGCAC G^TGCAC
1856 GTGCAC
AvaI 1903 CCCGGG C^[CT]CG[GA]G
2078 CTCGAG
AvrII 2327 CCTAGG C^CTAGG
2555 CCTAGG
BbvCI 1788 CCTCAGC CCTCAGC(-5/-2)
2198 GCTGAGG
BciVI 294 GGATAC GTATCC(6/5)
2828 GGATAC
BglI 1582 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1924 GCCCGCAGGGC
BlnI 2327 CCTAGG C^CTAGG
2555 CCTAGG
BsaI 1048 GAGACC GGTCTC(1/5)
2083 GAGACC
BsoBI 1903 CCCGGG C^[CT]CG[GA]G
2078 CTCGAG
BspHI 1004 TCATGA T^CATGA
1364 TCATGA
BsrDI 1731 CATTGC GCAATG(2/0)
710 GCAATG
BstXI 251 CCATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
986 CCAACCCACTGG
HaeII 924 GGCGCT [GA]GCGC^[CT]
1350 AGCGCT
MaeII 587 ACGT A^CGT
2149 ACGT
NcoI 309 CCATGG C^CATGG
2262 CCATGG
RcaI 1004 TCATGA T^CATGA
1364 TCATGA
SacI 1246 GAGCTC GAGCT^C
1480 GAGCTC
SapI 1161 GCTCTTC GCTCTTC(1/4)
2442 GCTCTTC
SbfI 1254 CCTGCAGG CCTGCA^GG
2392 CCTGCAGG
SphI 1113 GCATGC GCATG^C
1434 GCATGC
Sse8387I 1254 CCTGCAGG CCTGCA^GG
2392 CCTGCAGG
TaqI 1674 TCGA T^CGA
2079 TCGA
TfiI 181 GATTC G^A[AT]TC
976 GAATC
TthHB8I 1674 TCGA T^CGA
2079 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI Tru9I
TseI Tsp45I TspRI Van91I XhoII