################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI BbeI BcgI BclI BfrI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AocI 238 CCTCAGG CC^T[ACGT]AGG Aor51HI 1350 AGCGCT AGC^GCT Asp700I 265 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 2893 GGACC G^G[AT]CC AviII 707 TGCGCA TGC^GCA BbrPI 586 CACGTG CAC^GTG BglII 906 AGATCT A^GATCT BsaAI 586 CACGTG [CT]AC^GT[GA] BsaBI 1356 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspMI 1257 GCAGGT ACCTGC(4/8) BsrBI 757 GAGCGG CCGCTC(-3/-3) BsrFI 1621 GCCGGT [GA]^CCGG[CT] BstEII 1404 GGTCACC G^GT[ACGT]ACC BstPI 1404 GGTCACC G^GT[ACGT]ACC Bsu36I 238 CCTCAGG CC^T[ACGT]AGG Cfr10I 1621 GCCGGT [GA]^CCGG[CT] DraI 3166 TTTAAA TTT^AAA DrdI 3053 GACGCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1350 AGCGCT AGC^GCT Eco81I 238 CCTCAGG CC^T[ACGT]AGG EcoO65I 1404 GGTCACC G^GT[ACGT]ACC FspI 707 TGCGCA TGC^GCA HincII 1762 GTCAAC GT[CT]^[GA]AC HindII 1762 GTCAAC GT[CT]^[GA]AC MamI 1356 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NheI 1710 GCTAGC G^CTAGC PaeR7I 2078 CTCGAG C^TCGAG PmaCI 586 CACGTG CAC^GTG PmlI 586 CACGTG CAC^GTG PpuMI 2892 GGGACCC [GA]G^G[AT]CC[CT] SmaI 1903 CCCGGG CCC^GGG SpeI 2818 ACTAGT A^CTAGT Tsp509I 1814 AATT ^AATT XcmI 1706 CCACGCTAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2078 CTCGAG C^TCGAG XmaCI 1903 CCCGGG C^CCGGG XmaI 1903 CCCGGG C^CCGGG XmnI 265 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Alw44I 284 GTGCAC G^TGCAC 1856 GTGCAC ApaLI 284 GTGCAC G^TGCAC 1856 GTGCAC AvaI 1903 CCCGGG C^[CT]CG[GA]G 2078 CTCGAG AvrII 2327 CCTAGG C^CTAGG 2555 CCTAGG BbvCI 1788 CCTCAGC CCTCAGC(-5/-2) 2198 GCTGAGG BciVI 294 GGATAC GTATCC(6/5) 2828 GGATAC BglI 1582 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1924 GCCCGCAGGGC BlnI 2327 CCTAGG C^CTAGG 2555 CCTAGG BsaI 1048 GAGACC GGTCTC(1/5) 2083 GAGACC BsoBI 1903 CCCGGG C^[CT]CG[GA]G 2078 CTCGAG BspHI 1004 TCATGA T^CATGA 1364 TCATGA BsrDI 1731 CATTGC GCAATG(2/0) 710 GCAATG BstXI 251 CCATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 986 CCAACCCACTGG HaeII 924 GGCGCT [GA]GCGC^[CT] 1350 AGCGCT MaeII 587 ACGT A^CGT 2149 ACGT NcoI 309 CCATGG C^CATGG 2262 CCATGG RcaI 1004 TCATGA T^CATGA 1364 TCATGA SacI 1246 GAGCTC GAGCT^C 1480 GAGCTC SapI 1161 GCTCTTC GCTCTTC(1/4) 2442 GCTCTTC SbfI 1254 CCTGCAGG CCTGCA^GG 2392 CCTGCAGG SphI 1113 GCATGC GCATG^C 1434 GCATGC Sse8387I 1254 CCTGCAGG CCTGCA^GG 2392 CCTGCAGG TaqI 1674 TCGA T^CGA 2079 TCGA TfiI 181 GATTC G^A[AT]TC 976 GAATC TthHB8I 1674 TCGA T^CGA 2079 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI Tru9I TseI Tsp45I TspRI Van91I XhoII