##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI BalI
BamHI BcgI BciVI BfrI BlpI
Bpu1102I BsaWI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FseI HincII HindII
HindIII HpaI KpnI MfeI MluI
MluNI MroI MscI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 173 GACGTC GACGT^C
AcsI 543 GAATTT [GA]^AATT[CT]
Alw44I 2496 GTGCAC G^TGCAC
AocI 162 CCTCAGG CC^T[ACGT]AGG
ApaI 1140 GGGCCC GGGCC^C
ApaLI 2496 GTGCAC G^TGCAC
ApoI 543 GAATTT [GA]^AATT[CT]
Asp700I 1922 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1348 TGCGCA TGC^GCA
AvrII 2755 CCTAGG C^CTAGG
BbrPI 1102 CACGTG CAC^GTG
BbsI 1004 GTCTTC GAAGAC(2/6)
BglII 310 AGATCT A^GATCT
BlnI 2755 CCTAGG C^CTAGG
BpuAI 1004 GTCTTC GAAGAC(2/6)
BsaAI 1102 CACGTG [CT]AC^GT[GA]
BsaBI 1796 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 69 GAGACC GGTCTC(1/5)
BsiEI 128 CGACCG CG[GA][CT]^CG
BsmI 628 GAATGC GAATGC(1/-1)
BspHI 2575 TCATGA T^CATGA
BsrDI 1295 CATTGC GCAATG(2/0)
BstYI 310 AGATCT [GA]^GATC[CT]
Bsu36I 162 CCTCAGG CC^T[ACGT]AGG
Eco81I 162 CCTCAGG CC^T[ACGT]AGG
EcoRV 1776 GATATC GAT^ATC
FspI 1348 TGCGCA TGC^GCA
MamI 1796 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 310 AGATCT [GA]^GATC[CT]
NcoI 958 CCATGG C^CATGG
PflMI 816 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1102 CACGTG CAC^GTG
PmlI 1102 CACGTG CAC^GTG
RcaI 2575 TCATGA T^CATGA
SacI 1740 GAGCTC GAGCT^C
SapI 1373 GCTCTTC GCTCTTC(1/4)
SmaI 688 CCCGGG CCC^GGG
Van91I 816 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 614 CCACCAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 310 AGATCT [GA]^GATC[CT]
XmaCI 688 CCCGGG C^CCGGG
XmaI 688 CCCGGG C^CCGGG
XmnI 1922 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 553 ACATGT A^C[GA][CT]GT
2247 ACATGT
AlwI 1865 GATCC GGATC(4/5)
1934 GGATC
BbeI 135 GGCGCC GGCGC^C
1488 GGCGCC
BclI 356 TGATCA T^GATCA
1390 TGATCA
BpmI 472 CTGGAG CTGGAG(16/14)
633 CTGGAG
BsgI 1899 CTGCAC GTGCAG(16/14)
2677 CTGCAC
Bsp1407I 341 TGTACA T^GTACA
1429 TGTACA
BspLU11I 553 ACATGT A^CATGT
2247 ACATGT
BspMI 602 ACCTGC ACCTGC(4/8)
1897 ACCTGC
BsrBI 994 CCGCTC CCGCTC(-3/-3)
639 GAGCGG
BsrFI 1405 ACCGGC [GA]^CCGG[CT]
2130 ACCGGC
BsrGI 341 TGTACA T^GTACA
1429 TGTACA
BssSI 1410 CACGAG CACGAG(-5/-1)
2493 CTCGTG
BstXI 617 CCAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
982 CCACAGGCCTGG
Cfr10I 1405 ACCGGC [GA]^CCGG[CT]
2130 ACCGGC
EarI 1374 CTCTTC CTCTTC(1/4)
2587 GAAGAG
FbaI 356 TGATCA T^GATCA
1390 TGATCA
HgaI 563 GACGC GACGC(5/10)
1908 GCGTC
KasI 135 GGCGCC G^GCGCC
1488 GGCGCC
Ksp632I 1374 CTCTTC CTCTTC(1/4)
2587 GAAGAG
MseI 895 TTAA T^TAA
2870 TTAA
MslI 554 CATGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1514 CACCAGGATG
NarI 135 GGCGCC GG^CGCC
1488 GGCGCC
NspI 553 ACATGT [GA]CATG^[CT]
2247 ACATGT
PleI 1617 GACTC GAGTC(4/5)
590 GAGTC
PvuII 880 CAGCTG CAG^CTG
1212 CAGCTG
SexAI 216 ACCAGGT A^CC[AT]GGT
1441 ACCTGGT
SfoI 135 GGCGCC GGC^GCC
1488 GGCGCC
SmlI 234 CTCAAG C^T[CT][GA]AG
2852 CTCAAG
SspBI 341 TGTACA T^GTACA
1429 TGTACA
StuI 796 AGGCCT AGG^CCT
986 AGGCCT
Tru9I 895 TTAA T^TAA
2870 TTAA
Tsp509I 544 AATT ^AATT
2868 AATT
XbaI 1247 TCTAGA T^CTAGA
2700 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I