##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BbeI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CfoI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FseI FspI HaeII HhaI
HinP1I KasI KspI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 617 GGTACC G^GTACC
AccI 821 GTCTAC GT^[AC][GT]AC
AccII 40 CGCG CG^CG
AcsI 58 GAATTC [GA]^AATT[CT]
AcyI 1275 GACGCC G[GA]^CG[CT]C
AflII 2282 CTTAAG C^TTAAG
Alw44I 2437 GTGCAC G^TGCAC
ApaI 698 GGGCCC GGGCC^C
ApaLI 2437 GTGCAC G^TGCAC
ApoI 58 GAATTC [GA]^AATT[CT]
Asp718I 617 GGTACC G^GTACC
AspI 427 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2358 CTCGAG C^[CT]CG[GA]G
BbrPI 1615 CACGTG CAC^GTG
BbsI 1 GAAGAC GAAGAC(2/6)
BcgI 577 CGACACCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 773 GTATCC GTATCC(6/5)
BclI 1510 TGATCA T^GATCA
BfrI 2282 CTTAAG C^TTAAG
BglII 452 AGATCT A^GATCT
BpuAI 1 GAAGAC GAAGAC(2/6)
BsaAI 1615 CACGTG [CT]AC^GT[GA]
BsaHI 1275 GACGCC G[GA]^CG[CT]C
BsmBI 1273 GAGACG CGTCTC(1/5)
BsmI 2425 GAATGC GAATGC(1/-1)
BsoBI 2358 CTCGAG C^[CT]CG[GA]G
Bsp1407I 140 TGTACA T^GTACA
BsrBI 1071 GAGCGG CCGCTC(-3/-3)
BsrDI 1412 CATTGC GCAATG(2/0)
BsrFI 644 GCCGGC [GA]^CCGG[CT]
BsrGI 140 TGTACA T^GTACA
BstEII 1839 GGTGACC G^GT[ACGT]ACC
BstPI 1839 GGTGACC G^GT[ACGT]ACC
BstUI 40 CGCG CG^CG
Cfr10I 644 GCCGGC [GA]^CCGG[CT]
EarI 2203 GAAGAG CTCTTC(1/4)
EcoO65I 1839 GGTGACC G^GT[ACGT]ACC
EcoRI 58 GAATTC G^AATTC
FbaI 1510 TGATCA T^GATCA
Hin1I 1275 GACGCC G[GA]^CG[CT]C
HincII 1936 GTTAAC GT[CT]^[GA]AC
HindII 1936 GTTAAC GT[CT]^[GA]AC
HindIII 2119 AAGCTT A^AGCTT
HpaI 1936 GTTAAC GTT^AAC
KpnI 617 GGTACC GGTAC^C
Ksp632I 2203 GAAGAG CTCTTC(1/4)
MaeII 1616 ACGT A^CGT
MvnI 40 CGCG CG^CG
NaeI 644 GCCGGC GCC^GGC
NdeI 2432 CATATG CA^TATG
NgoMIV 644 GCCGGC G^CCGGC
PaeR7I 2358 CTCGAG C^TCGAG
PflFI 427 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1615 CACGTG CAC^GTG
PmlI 1615 CACGTG CAC^GTG
SacI 2361 GAGCTC GAGCT^C
ScaI 2520 AGTACT AGT^ACT
SpeI 1890 ACTAGT A^CTAGT
SphI 2457 GCATGC GCATG^C
SspBI 140 TGTACA T^GTACA
StuI 1917 AGGCCT AGG^CCT
Tth111I 427 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1437 CCAGCGAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2358 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 2443 ACATGT A^C[GA][CT]GT
2570 ACATGT
AlwNI 1746 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1983 CAGGTCCTG
BalI 339 TGGCCA TGG^CCA
1476 TGGCCA
BbvCI 1397 CCTCAGC CCTCAGC(-5/-2)
2276 CCTCAGC
BglI 79 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2015 GCCAAGGTGGC
BlpI 1348 GCTGAGC GC^T[ACGT]AGC
1376 GCTTAGC
BpmI 980 CTCCAG CTGGAG(16/14)
1340 CTGGAG
Bpu1102I 1348 GCTGAGC GC^T[ACGT]AGC
1376 GCTTAGC
BspLU11I 2443 ACATGT A^CATGT
2570 ACATGT
CelII 1348 GCTGAGC GC^T[ACGT]AGC
1376 GCTTAGC
MluNI 339 TGGCCA TGG^CCA
1476 TGGCCA
MscI 339 TGGCCA TGG^CCA
1476 TGGCCA
PflMI 300 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
735 CCAAAGTGTGG
PpuMI 1984 AGGTCCT [GA]G^G[AT]CC[CT]
2272 GGGACCT
PstI 445 CTGCAG CTGCA^G
667 CTGCAG
PvuII 144 CAGCTG CAG^CTG
1905 CAGCTG
TfiI 935 GAATC G^A[AT]TC
1686 GAATC
Van91I 300 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
735 CCAAAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII