################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII AccIII AclI AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BbeI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FseI FspI HaeII HhaI HinP1I KasI KspI MamI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 617 GGTACC G^GTACC AccI 821 GTCTAC GT^[AC][GT]AC AccII 40 CGCG CG^CG AcsI 58 GAATTC [GA]^AATT[CT] AcyI 1275 GACGCC G[GA]^CG[CT]C AflII 2282 CTTAAG C^TTAAG Alw44I 2437 GTGCAC G^TGCAC ApaI 698 GGGCCC GGGCC^C ApaLI 2437 GTGCAC G^TGCAC ApoI 58 GAATTC [GA]^AATT[CT] Asp718I 617 GGTACC G^GTACC AspI 427 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2358 CTCGAG C^[CT]CG[GA]G BbrPI 1615 CACGTG CAC^GTG BbsI 1 GAAGAC GAAGAC(2/6) BcgI 577 CGACACCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 773 GTATCC GTATCC(6/5) BclI 1510 TGATCA T^GATCA BfrI 2282 CTTAAG C^TTAAG BglII 452 AGATCT A^GATCT BpuAI 1 GAAGAC GAAGAC(2/6) BsaAI 1615 CACGTG [CT]AC^GT[GA] BsaHI 1275 GACGCC G[GA]^CG[CT]C BsmBI 1273 GAGACG CGTCTC(1/5) BsmI 2425 GAATGC GAATGC(1/-1) BsoBI 2358 CTCGAG C^[CT]CG[GA]G Bsp1407I 140 TGTACA T^GTACA BsrBI 1071 GAGCGG CCGCTC(-3/-3) BsrDI 1412 CATTGC GCAATG(2/0) BsrFI 644 GCCGGC [GA]^CCGG[CT] BsrGI 140 TGTACA T^GTACA BstEII 1839 GGTGACC G^GT[ACGT]ACC BstPI 1839 GGTGACC G^GT[ACGT]ACC BstUI 40 CGCG CG^CG Cfr10I 644 GCCGGC [GA]^CCGG[CT] EarI 2203 GAAGAG CTCTTC(1/4) EcoO65I 1839 GGTGACC G^GT[ACGT]ACC EcoRI 58 GAATTC G^AATTC FbaI 1510 TGATCA T^GATCA Hin1I 1275 GACGCC G[GA]^CG[CT]C HincII 1936 GTTAAC GT[CT]^[GA]AC HindII 1936 GTTAAC GT[CT]^[GA]AC HindIII 2119 AAGCTT A^AGCTT HpaI 1936 GTTAAC GTT^AAC KpnI 617 GGTACC GGTAC^C Ksp632I 2203 GAAGAG CTCTTC(1/4) MaeII 1616 ACGT A^CGT MvnI 40 CGCG CG^CG NaeI 644 GCCGGC GCC^GGC NdeI 2432 CATATG CA^TATG NgoMIV 644 GCCGGC G^CCGGC PaeR7I 2358 CTCGAG C^TCGAG PflFI 427 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1615 CACGTG CAC^GTG PmlI 1615 CACGTG CAC^GTG SacI 2361 GAGCTC GAGCT^C ScaI 2520 AGTACT AGT^ACT SpeI 1890 ACTAGT A^CTAGT SphI 2457 GCATGC GCATG^C SspBI 140 TGTACA T^GTACA StuI 1917 AGGCCT AGG^CCT Tth111I 427 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1437 CCAGCGAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2358 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 2443 ACATGT A^C[GA][CT]GT 2570 ACATGT AlwNI 1746 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1983 CAGGTCCTG BalI 339 TGGCCA TGG^CCA 1476 TGGCCA BbvCI 1397 CCTCAGC CCTCAGC(-5/-2) 2276 CCTCAGC BglI 79 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2015 GCCAAGGTGGC BlpI 1348 GCTGAGC GC^T[ACGT]AGC 1376 GCTTAGC BpmI 980 CTCCAG CTGGAG(16/14) 1340 CTGGAG Bpu1102I 1348 GCTGAGC GC^T[ACGT]AGC 1376 GCTTAGC BspLU11I 2443 ACATGT A^CATGT 2570 ACATGT CelII 1348 GCTGAGC GC^T[ACGT]AGC 1376 GCTTAGC MluNI 339 TGGCCA TGG^CCA 1476 TGGCCA MscI 339 TGGCCA TGG^CCA 1476 TGGCCA PflMI 300 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 735 CCAAAGTGTGG PpuMI 1984 AGGTCCT [GA]G^G[AT]CC[CT] 2272 GGGACCT PstI 445 CTGCAG CTGCA^G 667 CTGCAG PvuII 144 CAGCTG CAG^CTG 1905 CAGCTG TfiI 935 GAATC G^A[AT]TC 1686 GAATC Van91I 300 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 735 CCAAAGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII