##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BamHI BanI
BbeI BbrPI BbvCI BglI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI Hin1I
KasI KpnI KspI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI StuI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 2740 CTTAAG C^TTAAG
AocI 3456 CCTAAGG CC^T[ACGT]AGG
AspI 1829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1815 CCTAGG C^CTAGG
BciVI 762 GGATAC GTATCC(6/5)
BclI 3170 TGATCA T^GATCA
BfrI 2740 CTTAAG C^TTAAG
BglII 2182 AGATCT A^GATCT
BlnI 1815 CCTAGG C^CTAGG
BsaBI 442 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1279 GAGGAG GAGGAG(10/8)
BsrBI 1223 GAGCGG CCGCTC(-3/-3)
BssSI 1768 CTCGTG CACGAG(-5/-1)
BstAPI 2345 GCACTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3456 CCTAAGG CC^T[ACGT]AGG
CpoI 1244 CGGTCCG CG^G[AT]CCG
Eco81I 3456 CCTAAGG CC^T[ACGT]AGG
EcoNI 287 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2319 GAATTC G^AATTC
FauI 384 CCCGC CCCGC(4/6)
FbaI 3170 TGATCA T^GATCA
HaeII 1806 GGCGCT [GA]GCGC^[CT]
MaeII 3250 ACGT A^CGT
MamI 442 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2568 GCCGGC GCC^GGC
NgoMIV 2568 GCCGGC G^CCGGC
NheI 3358 GCTAGC G^CTAGC
PaeR7I 1892 CTCGAG C^TCGAG
PflFI 1829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2125 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 124 CAGCTG CAG^CTG
RsrII 1244 CGGTCCG CG^G[AT]CCG
SbfI 1855 CCTGCAGG CCTGCA^GG
SpeI 1191 ACTAGT A^CTAGT
Sse8387I 1855 CCTGCAGG CCTGCA^GG
SspI 2076 AATATT AAT^ATT
Tth111I 1829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2125 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1491 TCTAGA T^CTAGA
XcmI 2362 CCAGCAGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1892 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1644 GTAGAC GT^[AC][GT]AC
2210 GTCTAC
AlwNI 124 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2870 CAGGCTCTG
Asp700I 1070 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3256 GAAAATGTTC
BanII 231 GGGCTC G[GA]GC[CT]^C
2635 GGGCTC
BcnI 1253 CCGGG CC^[GC]GG
1460 CCCGG
BlpI 129 GCTGAGC GC^T[ACGT]AGC
2400 GCTCAGC
BpmI 1218 CTCCAG CTGGAG(16/14)
1673 CTGGAG
Bpu1102I 129 GCTGAGC GC^T[ACGT]AGC
2400 GCTCAGC
BsmBI 388 CGTCTC CGTCTC(1/5)
1089 CGTCTC
BsmI 545 GCATTC GAATGC(1/-1)
2405 GCATTC
BsrFI 1841 GCCGGT [GA]^CCGG[CT]
2568 GCCGGC
BstEII 1096 GGTGACC G^GT[ACGT]ACC
1826 GGTGACC
BstPI 1096 GGTGACC G^GT[ACGT]ACC
1826 GGTGACC
BstYI 2182 AGATCT [GA]^GATC[CT]
3152 GGATCT
CelII 129 GCTGAGC GC^T[ACGT]AGC
2400 GCTCAGC
Cfr10I 1841 GCCGGT [GA]^CCGG[CT]
2568 GCCGGC
DraII 295 AGGACCT [GA]G^G[ACGT]CC[CT]
3452 AGGACCT
DraIII 2530 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
3372 CACTCCGTG
DrdI 406 GACACGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3430 GACACTGCTGTC
EcoO109I 295 AGGACCT [GA]G^G[ACGT]CC[CT]
3452 AGGACCT
EcoO65I 1096 GGTGACC G^GT[ACGT]ACC
1826 GGTGACC
HgaI 387 GCGTC GACGC(5/10)
1088 GCGTC
HincII 2103 GTTAAC GT[CT]^[GA]AC
3246 GTTAAC
HindII 2103 GTTAAC GT[CT]^[GA]AC
3246 GTTAAC
HindIII 513 AAGCTT A^AGCTT
1872 AAGCTT
HpaI 2103 GTTAAC GTT^AAC
3246 GTTAAC
MflI 2182 AGATCT [GA]^GATC[CT]
3152 GGATCT
MspA1I 124 CAGCTG C[AC]G^C[GT]G
706 CAGCGG
NciI 1253 CCGGG CC^[GC]GG
1460 CCCGG
NcoI 647 CCATGG C^CATGG
1124 CCATGG
PpuMI 295 AGGACCT [GA]G^G[AT]CC[CT]
3452 AGGACCT
SapI 1809 GCTCTTC GCTCTTC(1/4)
3387 GCTCTTC
ScaI 621 AGTACT AGT^ACT
3283 AGTACT
SmlI 1892 CTCGAG C^T[CT][GA]AG
2740 CTTAAG
SphI 485 GCATGC GCATG^C
875 GCATGC
TaqI 279 TCGA T^CGA
1893 TCGA
TthHB8I 279 TCGA T^CGA
1893 TCGA
XhoII 2182 AGATCT [GA]^GATC[CT]
3152 GGATCT
XmnI 1070 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3256 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BbsI BbvI BcgI BfaI
BmyI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI