################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII BamHI BanI BbeI BbrPI BbvCI BglI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI Hin1I KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflII 2740 CTTAAG C^TTAAG AocI 3456 CCTAAGG CC^T[ACGT]AGG AspI 1829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1815 CCTAGG C^CTAGG BciVI 762 GGATAC GTATCC(6/5) BclI 3170 TGATCA T^GATCA BfrI 2740 CTTAAG C^TTAAG BglII 2182 AGATCT A^GATCT BlnI 1815 CCTAGG C^CTAGG BsaBI 442 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 1279 GAGGAG GAGGAG(10/8) BsrBI 1223 GAGCGG CCGCTC(-3/-3) BssSI 1768 CTCGTG CACGAG(-5/-1) BstAPI 2345 GCACTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3456 CCTAAGG CC^T[ACGT]AGG CpoI 1244 CGGTCCG CG^G[AT]CCG Eco81I 3456 CCTAAGG CC^T[ACGT]AGG EcoNI 287 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2319 GAATTC G^AATTC FauI 384 CCCGC CCCGC(4/6) FbaI 3170 TGATCA T^GATCA HaeII 1806 GGCGCT [GA]GCGC^[CT] MaeII 3250 ACGT A^CGT MamI 442 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2568 GCCGGC GCC^GGC NgoMIV 2568 GCCGGC G^CCGGC NheI 3358 GCTAGC G^CTAGC PaeR7I 1892 CTCGAG C^TCGAG PflFI 1829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2125 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 124 CAGCTG CAG^CTG RsrII 1244 CGGTCCG CG^G[AT]CCG SbfI 1855 CCTGCAGG CCTGCA^GG SpeI 1191 ACTAGT A^CTAGT Sse8387I 1855 CCTGCAGG CCTGCA^GG SspI 2076 AATATT AAT^ATT Tth111I 1829 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2125 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1491 TCTAGA T^CTAGA XcmI 2362 CCAGCAGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1892 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 1644 GTAGAC GT^[AC][GT]AC 2210 GTCTAC AlwNI 124 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 2870 CAGGCTCTG Asp700I 1070 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3256 GAAAATGTTC BanII 231 GGGCTC G[GA]GC[CT]^C 2635 GGGCTC BcnI 1253 CCGGG CC^[GC]GG 1460 CCCGG BlpI 129 GCTGAGC GC^T[ACGT]AGC 2400 GCTCAGC BpmI 1218 CTCCAG CTGGAG(16/14) 1673 CTGGAG Bpu1102I 129 GCTGAGC GC^T[ACGT]AGC 2400 GCTCAGC BsmBI 388 CGTCTC CGTCTC(1/5) 1089 CGTCTC BsmI 545 GCATTC GAATGC(1/-1) 2405 GCATTC BsrFI 1841 GCCGGT [GA]^CCGG[CT] 2568 GCCGGC BstEII 1096 GGTGACC G^GT[ACGT]ACC 1826 GGTGACC BstPI 1096 GGTGACC G^GT[ACGT]ACC 1826 GGTGACC BstYI 2182 AGATCT [GA]^GATC[CT] 3152 GGATCT CelII 129 GCTGAGC GC^T[ACGT]AGC 2400 GCTCAGC Cfr10I 1841 GCCGGT [GA]^CCGG[CT] 2568 GCCGGC DraII 295 AGGACCT [GA]G^G[ACGT]CC[CT] 3452 AGGACCT DraIII 2530 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 3372 CACTCCGTG DrdI 406 GACACGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3430 GACACTGCTGTC EcoO109I 295 AGGACCT [GA]G^G[ACGT]CC[CT] 3452 AGGACCT EcoO65I 1096 GGTGACC G^GT[ACGT]ACC 1826 GGTGACC HgaI 387 GCGTC GACGC(5/10) 1088 GCGTC HincII 2103 GTTAAC GT[CT]^[GA]AC 3246 GTTAAC HindII 2103 GTTAAC GT[CT]^[GA]AC 3246 GTTAAC HindIII 513 AAGCTT A^AGCTT 1872 AAGCTT HpaI 2103 GTTAAC GTT^AAC 3246 GTTAAC MflI 2182 AGATCT [GA]^GATC[CT] 3152 GGATCT MspA1I 124 CAGCTG C[AC]G^C[GT]G 706 CAGCGG NciI 1253 CCGGG CC^[GC]GG 1460 CCCGG NcoI 647 CCATGG C^CATGG 1124 CCATGG PpuMI 295 AGGACCT [GA]G^G[AT]CC[CT] 3452 AGGACCT SapI 1809 GCTCTTC GCTCTTC(1/4) 3387 GCTCTTC ScaI 621 AGTACT AGT^ACT 3283 AGTACT SmlI 1892 CTCGAG C^T[CT][GA]AG 2740 CTTAAG SphI 485 GCATGC GCATG^C 875 GCATGC TaqI 279 TCGA T^CGA 1893 TCGA TthHB8I 279 TCGA T^CGA 1893 TCGA XhoII 2182 AGATCT [GA]^GATC[CT] 3152 GGATCT XmnI 1070 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3256 GAAAATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BalI BbsI BbvI BcgI BfaI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI