################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccII AclI AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BbrPI BclI BfrI BglII BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MamI MluI MslI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Van91I ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 543 TCCGGA T^CCGGA AcsI 1457 GAATTC [GA]^AATT[CT] AcyI 2635 GACGCC G[GA]^CG[CT]C AfaI 1585 GTAC GT^AC Aor51HI 849 AGCGCT AGC^GCT ApaI 116 GGGCCC GGGCC^C ApoI 1457 GAATTC [GA]^AATT[CT] Asp700I 1085 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1520 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 330 TGGCCA TGG^CCA BamHI 1071 GGATCC G^GATCC BanI 55 GGCACC G^G[CT][GA]CC BciVI 2154 GGATAC GTATCC(6/5) BglI 1274 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1985 CACGTA [CT]AC^GT[GA] BsaHI 2635 GACGCC G[GA]^CG[CT]C BsaWI 543 TCCGGA [AT]^CCGG[AT] BseAI 543 TCCGGA T^CCGGA BsiEI 351 CGGCCG CG[GA][CT]^CG BspEI 543 TCCGGA T^CCGGA BspLU11I 2861 ACATGT A^CATGT DraIII 2143 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 351 CGGCCG C^GGCCG EclXI 351 CGGCCG C^GGCCG Eco47III 849 AGCGCT AGC^GCT Eco52I 351 CGGCCG C^GGCCG EcoRI 1457 GAATTC G^AATTC HaeII 849 AGCGCT [GA]GCGC^[CT] Hin1I 2635 GACGCC G[GA]^CG[CT]C HindIII 2548 AAGCTT A^AGCTT MfeI 2714 CAATTG C^AATTG MluNI 330 TGGCCA TGG^CCA MroI 543 TCCGGA T^CCGGA MscI 330 TGGCCA TGG^CCA MunI 2714 CAATTG C^AATTG NheI 2186 GCTAGC G^CTAGC PaeR7I 2627 CTCGAG C^TCGAG PflFI 1520 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 1585 GTAC GT^AC StuI 732 AGGCCT AGG^CCT Tth111I 1520 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1723 TCTAGA T^CTAGA XhoI 2627 CTCGAG C^TCGAG XmnI 1085 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 2861 ACATGT A^C[GA][CT]GT 2898 ACGTGT BcgI 1939 CGATGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1147 GCAGCAGACTCG BsmBI 1157 CGTCTC CGTCTC(1/5) 1604 GAGACG BsrBI 909 GAGCGG CCGCTC(-3/-3) 1298 GAGCGG BstAPI 523 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1876 GCAGCTTCTGC BstEII 278 GGTGACC G^GT[ACGT]ACC 862 GGTCACC BstPI 278 GGTGACC G^GT[ACGT]ACC 862 GGTCACC BstXI 642 CCAGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1854 CCAAGGAGGTGG BstYI 540 AGATCC [GA]^GATC[CT] 1071 GGATCC CfoI 850 GCGC GCG^C 2140 GCGC EaeI 330 TGGCCA [CT]^GGCC[GA] 351 CGGCCG EcoO65I 278 GGTGACC G^GT[ACGT]ACC 862 GGTCACC FauI 591 CCCGC CCCGC(4/6) 1300 GCGGG HgaI 68 GACGC GACGC(5/10) 2635 GACGC HhaI 850 GCGC GCG^C 2140 GCGC HinP1I 850 GCGC G^CGC 2140 GCGC HincII 704 GTCAAC GT[CT]^[GA]AC 2257 GTTGAC HindII 704 GTCAAC GT[CT]^[GA]AC 2257 GTTGAC MflI 540 AGATCC [GA]^GATC[CT] 1071 GGATCC MseI 1871 TTAA T^TAA 2889 TTAA PpuMI 798 AGGTCCT [GA]G^G[AT]CC[CT] 2037 AGGACCC PshAI 157 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 235 GACTGGAGTC SacI 692 GAGCTC GAGCT^C 1208 GAGCTC SphI 486 GCATGC GCATG^C 1614 GCATGC TaqI 1083 TCGA T^CGA 2628 TCGA TfiI 220 GATTC G^A[AT]TC 1889 GAATC Tru9I 1871 TTAA T^TAA 2889 TTAA TthHB8I 1083 TCGA T^CGA 2628 TCGA XcmI 1275 CCATGGAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1984 CCACGTAGAGGATGG XhoII 540 AGATCC [GA]^GATC[CT] 1071 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CelII Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TseI Tsp45I Tsp509I TspRI XmaCI XmaI