##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MamI MluI MslI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 543 TCCGGA T^CCGGA
AcsI 1457 GAATTC [GA]^AATT[CT]
AcyI 2635 GACGCC G[GA]^CG[CT]C
AfaI 1585 GTAC GT^AC
Aor51HI 849 AGCGCT AGC^GCT
ApaI 116 GGGCCC GGGCC^C
ApoI 1457 GAATTC [GA]^AATT[CT]
Asp700I 1085 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1520 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 330 TGGCCA TGG^CCA
BamHI 1071 GGATCC G^GATCC
BanI 55 GGCACC G^G[CT][GA]CC
BciVI 2154 GGATAC GTATCC(6/5)
BglI 1274 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1985 CACGTA [CT]AC^GT[GA]
BsaHI 2635 GACGCC G[GA]^CG[CT]C
BsaWI 543 TCCGGA [AT]^CCGG[AT]
BseAI 543 TCCGGA T^CCGGA
BsiEI 351 CGGCCG CG[GA][CT]^CG
BspEI 543 TCCGGA T^CCGGA
BspLU11I 2861 ACATGT A^CATGT
DraIII 2143 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 351 CGGCCG C^GGCCG
EclXI 351 CGGCCG C^GGCCG
Eco47III 849 AGCGCT AGC^GCT
Eco52I 351 CGGCCG C^GGCCG
EcoRI 1457 GAATTC G^AATTC
HaeII 849 AGCGCT [GA]GCGC^[CT]
Hin1I 2635 GACGCC G[GA]^CG[CT]C
HindIII 2548 AAGCTT A^AGCTT
MfeI 2714 CAATTG C^AATTG
MluNI 330 TGGCCA TGG^CCA
MroI 543 TCCGGA T^CCGGA
MscI 330 TGGCCA TGG^CCA
MunI 2714 CAATTG C^AATTG
NheI 2186 GCTAGC G^CTAGC
PaeR7I 2627 CTCGAG C^TCGAG
PflFI 1520 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 1585 GTAC GT^AC
StuI 732 AGGCCT AGG^CCT
Tth111I 1520 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1723 TCTAGA T^CTAGA
XhoI 2627 CTCGAG C^TCGAG
XmnI 1085 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 2861 ACATGT A^C[GA][CT]GT
2898 ACGTGT
BcgI 1939 CGATGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1147 GCAGCAGACTCG
BsmBI 1157 CGTCTC CGTCTC(1/5)
1604 GAGACG
BsrBI 909 GAGCGG CCGCTC(-3/-3)
1298 GAGCGG
BstAPI 523 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1876 GCAGCTTCTGC
BstEII 278 GGTGACC G^GT[ACGT]ACC
862 GGTCACC
BstPI 278 GGTGACC G^GT[ACGT]ACC
862 GGTCACC
BstXI 642 CCAGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1854 CCAAGGAGGTGG
BstYI 540 AGATCC [GA]^GATC[CT]
1071 GGATCC
CfoI 850 GCGC GCG^C
2140 GCGC
EaeI 330 TGGCCA [CT]^GGCC[GA]
351 CGGCCG
EcoO65I 278 GGTGACC G^GT[ACGT]ACC
862 GGTCACC
FauI 591 CCCGC CCCGC(4/6)
1300 GCGGG
HgaI 68 GACGC GACGC(5/10)
2635 GACGC
HhaI 850 GCGC GCG^C
2140 GCGC
HinP1I 850 GCGC G^CGC
2140 GCGC
HincII 704 GTCAAC GT[CT]^[GA]AC
2257 GTTGAC
HindII 704 GTCAAC GT[CT]^[GA]AC
2257 GTTGAC
MflI 540 AGATCC [GA]^GATC[CT]
1071 GGATCC
MseI 1871 TTAA T^TAA
2889 TTAA
PpuMI 798 AGGTCCT [GA]G^G[AT]CC[CT]
2037 AGGACCC
PshAI 157 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
235 GACTGGAGTC
SacI 692 GAGCTC GAGCT^C
1208 GAGCTC
SphI 486 GCATGC GCATG^C
1614 GCATGC
TaqI 1083 TCGA T^CGA
2628 TCGA
TfiI 220 GATTC G^A[AT]TC
1889 GAATC
Tru9I 1871 TTAA T^TAA
2889 TTAA
TthHB8I 1083 TCGA T^CGA
2628 TCGA
XcmI 1275 CCATGGAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1984 CCACGTAGAGGATGG
XhoII 540 AGATCC [GA]^GATC[CT]
1071 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CelII Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TseI Tsp45I
Tsp509I TspRI XmaCI XmaI