##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BbeI        BbrPI       
BclI        BfrI        BglII       BlnI        BsaBI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DrdI        
Eam1105I    Eco81I      EcoRV       EcoT22I     FbaI        
FseI        FspI        HpaI        KasI        KpnI        
KspI        MamI        MluI        MslI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Van91I      


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII        543  TCCGGA               T^CCGGA
AcsI         1457  GAATTC               [GA]^AATT[CT]
AcyI         2635  GACGCC               G[GA]^CG[CT]C
AfaI         1585  GTAC                 GT^AC
Aor51HI       849  AGCGCT               AGC^GCT
ApaI          116  GGGCCC               GGGCC^C
ApoI         1457  GAATTC               [GA]^AATT[CT]
Asp700I      1085  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1520  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          330  TGGCCA               TGG^CCA
BamHI        1071  GGATCC               G^GATCC
BanI           55  GGCACC               G^G[CT][GA]CC
BciVI        2154  GGATAC               GTATCC(6/5)
BglI         1274  GCCATGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1985  CACGTA               [CT]AC^GT[GA]
BsaHI        2635  GACGCC               G[GA]^CG[CT]C
BsaWI         543  TCCGGA               [AT]^CCGG[AT]
BseAI         543  TCCGGA               T^CCGGA
BsiEI         351  CGGCCG               CG[GA][CT]^CG
BspEI         543  TCCGGA               T^CCGGA
BspLU11I     2861  ACATGT               A^CATGT
DraIII       2143  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          351  CGGCCG               C^GGCCG
EclXI         351  CGGCCG               C^GGCCG
Eco47III      849  AGCGCT               AGC^GCT
Eco52I        351  CGGCCG               C^GGCCG
EcoRI        1457  GAATTC               G^AATTC
HaeII         849  AGCGCT               [GA]GCGC^[CT]
Hin1I        2635  GACGCC               G[GA]^CG[CT]C
HindIII      2548  AAGCTT               A^AGCTT
MfeI         2714  CAATTG               C^AATTG
MluNI         330  TGGCCA               TGG^CCA
MroI          543  TCCGGA               T^CCGGA
MscI          330  TGGCCA               TGG^CCA
MunI         2714  CAATTG               C^AATTG
NheI         2186  GCTAGC               G^CTAGC
PaeR7I       2627  CTCGAG               C^TCGAG
PflFI        1520  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsaI         1585  GTAC                 GT^AC
StuI          732  AGGCCT               AGG^CCT
Tth111I      1520  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1723  TCTAGA               T^CTAGA
XhoI         2627  CTCGAG               C^TCGAG
XmnI         1085  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflIII       2861  ACATGT               A^C[GA][CT]GT
             2898  ACGTGT              
BcgI         1939  CGATGAGCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1147  GCAGCAGACTCG        
BsmBI        1157  CGTCTC               CGTCTC(1/5)
             1604  GAGACG              
BsrBI         909  GAGCGG               CCGCTC(-3/-3)
             1298  GAGCGG              
BstAPI        523  GCAACAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1876  GCAGCTTCTGC         
BstEII        278  GGTGACC              G^GT[ACGT]ACC
              862  GGTCACC             
BstPI         278  GGTGACC              G^GT[ACGT]ACC
              862  GGTCACC             
BstXI         642  CCAGCCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1854  CCAAGGAGGTGG        
BstYI         540  AGATCC               [GA]^GATC[CT]
             1071  GGATCC              
CfoI          850  GCGC                 GCG^C
             2140  GCGC                
EaeI          330  TGGCCA               [CT]^GGCC[GA]
              351  CGGCCG              
EcoO65I       278  GGTGACC              G^GT[ACGT]ACC
              862  GGTCACC             
FauI          591  CCCGC                CCCGC(4/6)
             1300  GCGGG               
HgaI           68  GACGC                GACGC(5/10)
             2635  GACGC               
HhaI          850  GCGC                 GCG^C
             2140  GCGC                
HinP1I        850  GCGC                 G^CGC
             2140  GCGC                
HincII        704  GTCAAC               GT[CT]^[GA]AC
             2257  GTTGAC              
HindII        704  GTCAAC               GT[CT]^[GA]AC
             2257  GTTGAC              
MflI          540  AGATCC               [GA]^GATC[CT]
             1071  GGATCC              
MseI         1871  TTAA                 T^TAA
             2889  TTAA                
PpuMI         798  AGGTCCT              [GA]G^G[AT]CC[CT]
             2037  AGGACCC             
PshAI         157  GACACTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              235  GACTGGAGTC          
SacI          692  GAGCTC               GAGCT^C
             1208  GAGCTC              
SphI          486  GCATGC               GCATG^C
             1614  GCATGC              
TaqI         1083  TCGA                 T^CGA
             2628  TCGA                
TfiI          220  GATTC                G^A[AT]TC
             1889  GAATC               
Tru9I        1871  TTAA                 T^TAA
             2889  TTAA                
TthHB8I      1083  TCGA                 T^CGA
             2628  TCGA                
XcmI         1275  CCATGGAGGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1984  CCACGTAGAGGATGG     
XhoII         540  AGATCC               [GA]^GATC[CT]
             1071  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AspHI       
AvaI        AvaII       BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CelII       Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StyI        TseI        Tsp45I      
Tsp509I     TspRI       XmaCI       XmaI