##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AflII AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
BbeI BbrPI BciVI BfrI BglI
BglII BsaAI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BstAPI ClaI DrdI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FauI FseI HindIII HpaI KasI
MluI MroI NarI NdeI NotI
NruI NsiI PacI PaeR7I PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
SalI ScaI SexAI SfiI SfoI
SgrAI SnaBI SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 1332 GACGTC GACGT^C
Acc65I 3147 GGTACC G^GTACC
AccII 1017 CGCG CG^CG
AflIII 987 ACGTGT A^C[GA][CT]GT
AgeI 1175 ACCGGT A^CCGGT
AlwNI 947 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3266 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1784 AGCGCT AGC^GCT
Asp718I 3147 GGTACC G^GTACC
AspI 3350 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2709 TGCGCA TGC^GCA
BclI 51 TGATCA T^GATCA
BlpI 502 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 502 GCTCAGC GC^T[ACGT]AGC
BsaBI 622 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3376 GAGACC GGTCTC(1/5)
BsaWI 1175 ACCGGT [AT]^CCGG[AT]
BsiEI 1636 CGATCG CG[GA][CT]^CG
BsmBI 2093 CGTCTC CGTCTC(1/5)
BsmI 869 GCATTC GAATGC(1/-1)
BsrDI 2100 GCAATG GCAATG(2/0)
BssSI 761 CTCGTG CACGAG(-5/-1)
Bst1107I 227 GTATAC GTA^TAC
BstBI 410 TTCGAA TT^CGAA
BstEII 1036 GGTCACC G^GT[ACGT]ACC
BstPI 1036 GGTCACC G^GT[ACGT]ACC
BstUI 1017 CGCG CG^CG
BstZ17I 227 GTATAC GTA^TAC
Bsu36I 3266 CCTCAGG CC^T[ACGT]AGG
CelII 502 GCTCAGC GC^T[ACGT]AGC
CpoI 338 CGGACCG CG^G[AT]CCG
Eco47III 1784 AGCGCT AGC^GCT
Eco81I 3266 CCTCAGG CC^T[ACGT]AGG
EcoNI 2076 CCTTAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1036 GGTCACC G^GT[ACGT]ACC
EcoRI 637 GAATTC G^AATTC
FbaI 51 TGATCA T^GATCA
FspI 2709 TGCGCA TGC^GCA
HaeII 1784 AGCGCT [GA]GCGC^[CT]
KpnI 3147 GGTACC GGTAC^C
KspI 1016 CCGCGG CCGC^GG
MamI 622 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1296 CAATTG C^AATTG
MunI 1296 CAATTG C^AATTG
MvnI 1017 CGCG CG^CG
NheI 3331 GCTAGC G^CTAGC
NspV 410 TTCGAA TT^CGAA
PflFI 3350 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1188 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1175 ACCGGT A^CCGGT
PshAI 1654 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1636 CGATCG CGAT^CG
PvuII 947 CAGCTG CAG^CTG
RsrII 338 CGGACCG CG^G[AT]CCG
SacII 1016 CCGCGG CCGC^GG
SbfI 2247 CCTGCAGG CCTGCA^GG
SfuI 410 TTCGAA TT^CGAA
SpeI 3436 ACTAGT A^CTAGT
Sse8387I 2247 CCTGCAGG CCTGCA^GG
TfiI 2534 GATTC G^A[AT]TC
Tth111I 3350 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1188 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 227 GTATAC GT^[AC][GT]AC
1888 GTAGAC
Asp700I 511 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
844 GAACCTGTTC
AvrII 3158 CCTAGG C^CTAGG
3698 CCTAGG
BalI 1023 TGGCCA TGG^CCA
2931 TGGCCA
BbvCI 3152 CCTCAGC CCTCAGC(-5/-2)
952 GCTGAGG
BlnI 3158 CCTAGG C^CTAGG
3698 CCTAGG
BsgI 329 CTGCAC GTGCAG(16/14)
3631 GTGCAG
BspMI 261 ACCTGC ACCTGC(4/8)
2743 ACCTGC
DraI 962 TTTAAA TTT^AAA
2844 TTTAAA
MluNI 1023 TGGCCA TGG^CCA
2931 TGGCCA
MscI 1023 TGGCCA TGG^CCA
2931 TGGCCA
MspA1I 947 CAGCTG C[AC]G^C[GT]G
1016 CCGCGG
NaeI 385 GCCGGC GCC^GGC
2105 GCCGGC
NgoMIV 385 GCCGGC G^CCGGC
2105 GCCGGC
SapI 2305 GAAGAGC GCTCTTC(1/4)
40 GCTCTTC
SmlI 2065 CTCAAG C^T[CT][GA]AG
2292 CTTGAG
SphI 352 GCATGC GCATG^C
1704 GCATGC
XmnI 511 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
844 GAACCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvI BcgI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI