################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AccIII AclI AflII AhdI Alw44I ApaLI AscI AseI AsnI AspEI BbeI BbrPI BciVI BfrI BglI BglII BsaAI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BstAPI ClaI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FauI FseI HindIII HpaI KasI MluI MroI NarI NdeI NotI NruI NsiI PacI PaeR7I PmaCI PmeI PmlI PpuMI PshBI Psp1406I SalI ScaI SexAI SfiI SfoI SgrAI SnaBI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 1332 GACGTC GACGT^C Acc65I 3147 GGTACC G^GTACC AccII 1017 CGCG CG^CG AflIII 987 ACGTGT A^C[GA][CT]GT AgeI 1175 ACCGGT A^CCGGT AlwNI 947 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 3266 CCTCAGG CC^T[ACGT]AGG Aor51HI 1784 AGCGCT AGC^GCT Asp718I 3147 GGTACC G^GTACC AspI 3350 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2709 TGCGCA TGC^GCA BclI 51 TGATCA T^GATCA BlpI 502 GCTCAGC GC^T[ACGT]AGC Bpu1102I 502 GCTCAGC GC^T[ACGT]AGC BsaBI 622 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3376 GAGACC GGTCTC(1/5) BsaWI 1175 ACCGGT [AT]^CCGG[AT] BsiEI 1636 CGATCG CG[GA][CT]^CG BsmBI 2093 CGTCTC CGTCTC(1/5) BsmI 869 GCATTC GAATGC(1/-1) BsrDI 2100 GCAATG GCAATG(2/0) BssSI 761 CTCGTG CACGAG(-5/-1) Bst1107I 227 GTATAC GTA^TAC BstBI 410 TTCGAA TT^CGAA BstEII 1036 GGTCACC G^GT[ACGT]ACC BstPI 1036 GGTCACC G^GT[ACGT]ACC BstUI 1017 CGCG CG^CG BstZ17I 227 GTATAC GTA^TAC Bsu36I 3266 CCTCAGG CC^T[ACGT]AGG CelII 502 GCTCAGC GC^T[ACGT]AGC CpoI 338 CGGACCG CG^G[AT]CCG Eco47III 1784 AGCGCT AGC^GCT Eco81I 3266 CCTCAGG CC^T[ACGT]AGG EcoNI 2076 CCTTAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1036 GGTCACC G^GT[ACGT]ACC EcoRI 637 GAATTC G^AATTC FbaI 51 TGATCA T^GATCA FspI 2709 TGCGCA TGC^GCA HaeII 1784 AGCGCT [GA]GCGC^[CT] KpnI 3147 GGTACC GGTAC^C KspI 1016 CCGCGG CCGC^GG MamI 622 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1296 CAATTG C^AATTG MunI 1296 CAATTG C^AATTG MvnI 1017 CGCG CG^CG NheI 3331 GCTAGC G^CTAGC NspV 410 TTCGAA TT^CGAA PflFI 3350 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1188 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1175 ACCGGT A^CCGGT PshAI 1654 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1636 CGATCG CGAT^CG PvuII 947 CAGCTG CAG^CTG RsrII 338 CGGACCG CG^G[AT]CCG SacII 1016 CCGCGG CCGC^GG SbfI 2247 CCTGCAGG CCTGCA^GG SfuI 410 TTCGAA TT^CGAA SpeI 3436 ACTAGT A^CTAGT Sse8387I 2247 CCTGCAGG CCTGCA^GG TfiI 2534 GATTC G^A[AT]TC Tth111I 3350 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1188 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 227 GTATAC GT^[AC][GT]AC 1888 GTAGAC Asp700I 511 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 844 GAACCTGTTC AvrII 3158 CCTAGG C^CTAGG 3698 CCTAGG BalI 1023 TGGCCA TGG^CCA 2931 TGGCCA BbvCI 3152 CCTCAGC CCTCAGC(-5/-2) 952 GCTGAGG BlnI 3158 CCTAGG C^CTAGG 3698 CCTAGG BsgI 329 CTGCAC GTGCAG(16/14) 3631 GTGCAG BspMI 261 ACCTGC ACCTGC(4/8) 2743 ACCTGC DraI 962 TTTAAA TTT^AAA 2844 TTTAAA MluNI 1023 TGGCCA TGG^CCA 2931 TGGCCA MscI 1023 TGGCCA TGG^CCA 2931 TGGCCA MspA1I 947 CAGCTG C[AC]G^C[GT]G 1016 CCGCGG NaeI 385 GCCGGC GCC^GGC 2105 GCCGGC NgoMIV 385 GCCGGC G^CCGGC 2105 GCCGGC SapI 2305 GAAGAGC GCTCTTC(1/4) 40 GCTCTTC SmlI 2065 CTCAAG C^T[CT][GA]AG 2292 CTTGAG SphI 352 GCATGC GCATG^C 1704 GCATGC XmnI 511 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 844 GAACCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApaI ApoI AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI