##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrFI
BssHII BssSI BstAPI BstBI BstUI
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 3208 GTATAC GT^[AC][GT]AC
AcyI 1665 GACGCC G[GA]^CG[CT]C
AflII 2595 CTTAAG C^TTAAG
AflIII 184 ACATGT A^C[GA][CT]GT
AlwNI 723 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2083 CCTCAGG CC^T[ACGT]AGG
ApaI 898 GGGCCC GGGCC^C
AseI 3061 ATTAAT AT^TAAT
AsnI 3061 ATTAAT AT^TAAT
Asp700I 660 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1864 TGGCCA TGG^CCA
BanI 1751 GGCACC G^G[CT][GA]CC
BbsI 2805 GTCTTC GAAGAC(2/6)
BcgI 1183 GCAACATTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3243 TGATCA T^GATCA
BfrI 2595 CTTAAG C^TTAAG
BglI 891 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2805 GTCTTC GAAGAC(2/6)
BsaHI 1665 GACGCC G[GA]^CG[CT]C
BsmI 439 GAATGC GAATGC(1/-1)
BspLU11I 184 ACATGT A^CATGT
BsrDI 2838 CATTGC GCAATG(2/0)
Bst1107I 3208 GTATAC GTA^TAC
BstEII 1152 GGTGACC G^GT[ACGT]ACC
BstPI 1152 GGTGACC G^GT[ACGT]ACC
BstXI 2657 CCATGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3208 GTATAC GTA^TAC
Bsu36I 2083 CCTCAGG CC^T[ACGT]AGG
DraI 2980 TTTAAA TTT^AAA
DsaI 2221 CCATGG C^C[GA][CT]GG
EaeI 1864 TGGCCA [CT]^GGCC[GA]
Eco81I 2083 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1152 GGTGACC G^GT[ACGT]ACC
EcoT22I 1961 ATGCAT ATGCA^T
FbaI 3243 TGATCA T^GATCA
Hin1I 1665 GACGCC G[GA]^CG[CT]C
HindIII 219 AAGCTT A^AGCTT
MfeI 1689 CAATTG C^AATTG
MluNI 1864 TGGCCA TGG^CCA
MscI 1864 TGGCCA TGG^CCA
MslI 1406 CACACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1689 CAATTG C^AATTG
NcoI 2221 CCATGG C^CATGG
NheI 1049 GCTAGC G^CTAGC
NsiI 1961 ATGCAT ATGCA^T
PaeR7I 612 CTCGAG C^TCGAG
PshBI 3061 ATTAAT AT^TAAT
PvuII 1499 CAGCTG CAG^CTG
SapI 660 GAAGAGC GCTCTTC(1/4)
ScaI 1012 AGTACT AGT^ACT
SexAI 1069 ACCTGGT A^CC[AT]GGT
SfiI 890 GGCCCAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 2316 GATTC G^A[AT]TC
XbaI 1208 TCTAGA T^CTAGA
XhoI 612 CTCGAG C^TCGAG
XmnI 660 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspI 1068 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2152 GACCCTGTC
AvaI 612 CTCGAG C^[CT]CG[GA]G
1157 CCCGAG
BcnI 67 CCGGG CC^[GC]GG
932 CCGGG
BglII 1205 AGATCT A^GATCT
1512 AGATCT
BsaI 456 GAGACC GGTCTC(1/5)
1537 GAGACC
BsmBI 1663 GAGACG CGTCTC(1/5)
1812 GAGACG
BsoBI 612 CTCGAG C^[CT]CG[GA]G
1157 CCCGAG
BspHI 1672 TCATGA T^CATGA
2794 TCATGA
DraIII 1587 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3102 CACATCGTG
EcoRI 2006 GAATTC G^AATTC
2177 GAATTC
HaeII 1332 GGCGCT [GA]GCGC^[CT]
1938 GGCGCT
HapII 67 CCGG C^CGG
932 CCGG
HgaI 1143 GACGC GACGC(5/10)
1665 GACGC
HpaII 67 CCGG C^CGG
932 CCGG
MspI 67 CCGG C^CGG
932 CCGG
NciI 67 CCGGG CC^[GC]GG
932 CCGGG
PflFI 1068 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2152 GACCCTGTC
RcaI 1672 TCATGA T^CATGA
2794 TCATGA
SacI 681 GAGCTC GAGCT^C
1991 GAGCTC
Tth111I 1068 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2152 GACCCTGTC
XcmI 766 CCAAGCTGCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1356 CCACCCTGGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrBI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII