##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AhdI ApaI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BglII BlnI
BlpI Bpu1102I BsaHI BseAI BsiWI
BsmBI BsoBI BspEI BsrDI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I FbaI FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
Psp1406I PstI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 2046 CTTAAG C^TTAAG
AgeI 617 ACCGGT A^CCGGT
Alw44I 2456 GTGCAC G^TGCAC
AocI 2937 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1476 AGCGCT AGC^GCT
ApaLI 2456 GTGCAC G^TGCAC
AseI 232 ATTAAT AT^TAAT
AsnI 232 ATTAAT AT^TAAT
AvaII 531 GGACC G^G[AT]CC
BcnI 2547 CCGGG CC^[GC]GG
BfrI 2046 CTTAAG C^TTAAG
BsaAI 2250 TACGTG [CT]AC^GT[GA]
BsaBI 2352 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 986 GAGGAG GAGGAG(10/8)
BsiEI 651 CGATCG CG[GA][CT]^CG
BspDI 649 ATCGAT AT^CGAT
BspLU11I 860 ACATGT A^CATGT
BsrBI 1225 GAGCGG CCGCTC(-3/-3)
BssHII 11 GCGCGC G^CGCGC
Bsu36I 2937 CCTGAGG CC^T[ACGT]AGG
ClaI 649 ATCGAT AT^CGAT
DrdI 2905 GACTTCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1407 CCGTGG C^C[GA][CT]GG
EarI 84 CTCTTC CTCTTC(1/4)
Eco47III 1476 AGCGCT AGC^GCT
Eco81I 2937 CCTGAGG CC^T[ACGT]AGG
EcoNI 1015 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 224 GAATTC G^AATTC
EcoRV 1378 GATATC GAT^ATC
EcoT22I 2951 ATGCAT ATGCA^T
FauI 1227 GCGGG CCCGC(4/6)
HincII 808 GTTGAC GT[CT]^[GA]AC
HindII 808 GTTGAC GT[CT]^[GA]AC
HindIII 2043 AAGCTT A^AGCTT
Ksp632I 84 CTCTTC CTCTTC(1/4)
MamI 2352 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3000 CAATTG C^AATTG
MunI 3000 CAATTG C^AATTG
NciI 2547 CCGGG CC^[GC]GG
NsiI 2951 ATGCAT ATGCA^T
PinAI 617 ACCGGT A^CCGGT
PpuMI 530 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 232 ATTAAT AT^TAAT
PvuI 651 CGATCG CGAT^CG
SacI 898 GAGCTC GAGCT^C
SpeI 2243 ACTAGT A^CTAGT
SphI 2953 GCATGC GCATG^C
TfiI 576 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1580 GTAGAC GT^[AC][GT]AC
2524 GTCTAC
AccII 12 CGCG CG^CG
14 CGCG
AcsI 224 GAATTC [GA]^AATT[CT]
2675 GAATTT
AflIII 860 ACATGT A^C[GA][CT]GT
2251 ACGTGT
AlwNI 1740 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2154 CAGTTACTG
ApoI 224 GAATTC [GA]^AATT[CT]
2675 GAATTT
BalI 1751 TGGCCA TGG^CCA
2983 TGGCCA
BbsI 3057 GAAGAC GAAGAC(2/6)
2663 GTCTTC
BpuAI 3057 GAAGAC GAAGAC(2/6)
2663 GTCTTC
BsaWI 617 ACCGGT [AT]^CCGG[AT]
1523 TCCGGT
BsmI 1124 GCATTC GAATGC(1/-1)
2475 GCATTC
Bsp1407I 1073 TGTACA T^GTACA
1348 TGTACA
BspHI 579 TCATGA T^CATGA
1256 TCATGA
BsrFI 617 ACCGGT [GA]^CCGG[CT]
1514 ACCGGC
BsrGI 1073 TGTACA T^GTACA
1348 TGTACA
BssSI 1339 CACGAG CACGAG(-5/-1)
763 CTCGTG
BstUI 12 CGCG CG^CG
14 CGCG
BstXI 2435 CCAAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2888 CCAAATGTTTGG
Cfr10I 617 ACCGGT [GA]^CCGG[CT]
1514 ACCGGC
DraI 2919 TTTAAA TTT^AAA
3022 TTTAAA
EaeI 1751 TGGCCA [CT]^GGCC[GA]
2983 TGGCCA
EcoT14I 876 CCTTGG C^C[AT][AT]GG
2968 CCAAGG
HaeII 1476 AGCGCT [GA]GCGC^[CT]
1822 AGCGCC
MluNI 1751 TGGCCA TGG^CCA
2983 TGGCCA
MscI 1751 TGGCCA TGG^CCA
2983 TGGCCA
MspA1I 1004 CAGCTG C[AC]G^C[GT]G
2879 CAGCTG
MvnI 12 CGCG CG^CG
14 CGCG
NheI 1596 GCTAGC G^CTAGC
1600 GCTAGC
PvuII 1004 CAGCTG CAG^CTG
2879 CAGCTG
RcaI 579 TCATGA T^CATGA
1256 TCATGA
SspBI 1073 TGTACA T^GTACA
1348 TGTACA
StuI 1113 AGGCCT AGG^CCT
1276 AGGCCT
StyI 876 CCTTGG C^C[AT][AT]GG
2968 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanI BanII BbvI BfaI BmyI
BpmI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII