################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AhdI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaHI BseAI BsiWI BsmBI BsoBI BspEI BsrDI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII CpoI DraIII EagI Eam1105I EclXI Eco52I EcoO65I FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MluI MroI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI Psp1406I PstI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflII 2046 CTTAAG C^TTAAG AgeI 617 ACCGGT A^CCGGT Alw44I 2456 GTGCAC G^TGCAC AocI 2937 CCTGAGG CC^T[ACGT]AGG Aor51HI 1476 AGCGCT AGC^GCT ApaLI 2456 GTGCAC G^TGCAC AseI 232 ATTAAT AT^TAAT AsnI 232 ATTAAT AT^TAAT AvaII 531 GGACC G^G[AT]CC BcnI 2547 CCGGG CC^[GC]GG BfrI 2046 CTTAAG C^TTAAG BsaAI 2250 TACGTG [CT]AC^GT[GA] BsaBI 2352 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 986 GAGGAG GAGGAG(10/8) BsiEI 651 CGATCG CG[GA][CT]^CG BspDI 649 ATCGAT AT^CGAT BspLU11I 860 ACATGT A^CATGT BsrBI 1225 GAGCGG CCGCTC(-3/-3) BssHII 11 GCGCGC G^CGCGC Bsu36I 2937 CCTGAGG CC^T[ACGT]AGG ClaI 649 ATCGAT AT^CGAT DrdI 2905 GACTTCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1407 CCGTGG C^C[GA][CT]GG EarI 84 CTCTTC CTCTTC(1/4) Eco47III 1476 AGCGCT AGC^GCT Eco81I 2937 CCTGAGG CC^T[ACGT]AGG EcoNI 1015 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 224 GAATTC G^AATTC EcoRV 1378 GATATC GAT^ATC EcoT22I 2951 ATGCAT ATGCA^T FauI 1227 GCGGG CCCGC(4/6) HincII 808 GTTGAC GT[CT]^[GA]AC HindII 808 GTTGAC GT[CT]^[GA]AC HindIII 2043 AAGCTT A^AGCTT Ksp632I 84 CTCTTC CTCTTC(1/4) MamI 2352 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3000 CAATTG C^AATTG MunI 3000 CAATTG C^AATTG NciI 2547 CCGGG CC^[GC]GG NsiI 2951 ATGCAT ATGCA^T PinAI 617 ACCGGT A^CCGGT PpuMI 530 AGGACCT [GA]G^G[AT]CC[CT] PshBI 232 ATTAAT AT^TAAT PvuI 651 CGATCG CGAT^CG SacI 898 GAGCTC GAGCT^C SpeI 2243 ACTAGT A^CTAGT SphI 2953 GCATGC GCATG^C TfiI 576 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 1580 GTAGAC GT^[AC][GT]AC 2524 GTCTAC AccII 12 CGCG CG^CG 14 CGCG AcsI 224 GAATTC [GA]^AATT[CT] 2675 GAATTT AflIII 860 ACATGT A^C[GA][CT]GT 2251 ACGTGT AlwNI 1740 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 2154 CAGTTACTG ApoI 224 GAATTC [GA]^AATT[CT] 2675 GAATTT BalI 1751 TGGCCA TGG^CCA 2983 TGGCCA BbsI 3057 GAAGAC GAAGAC(2/6) 2663 GTCTTC BpuAI 3057 GAAGAC GAAGAC(2/6) 2663 GTCTTC BsaWI 617 ACCGGT [AT]^CCGG[AT] 1523 TCCGGT BsmI 1124 GCATTC GAATGC(1/-1) 2475 GCATTC Bsp1407I 1073 TGTACA T^GTACA 1348 TGTACA BspHI 579 TCATGA T^CATGA 1256 TCATGA BsrFI 617 ACCGGT [GA]^CCGG[CT] 1514 ACCGGC BsrGI 1073 TGTACA T^GTACA 1348 TGTACA BssSI 1339 CACGAG CACGAG(-5/-1) 763 CTCGTG BstUI 12 CGCG CG^CG 14 CGCG BstXI 2435 CCAAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2888 CCAAATGTTTGG Cfr10I 617 ACCGGT [GA]^CCGG[CT] 1514 ACCGGC DraI 2919 TTTAAA TTT^AAA 3022 TTTAAA EaeI 1751 TGGCCA [CT]^GGCC[GA] 2983 TGGCCA EcoT14I 876 CCTTGG C^C[AT][AT]GG 2968 CCAAGG HaeII 1476 AGCGCT [GA]GCGC^[CT] 1822 AGCGCC MluNI 1751 TGGCCA TGG^CCA 2983 TGGCCA MscI 1751 TGGCCA TGG^CCA 2983 TGGCCA MspA1I 1004 CAGCTG C[AC]G^C[GT]G 2879 CAGCTG MvnI 12 CGCG CG^CG 14 CGCG NheI 1596 GCTAGC G^CTAGC 1600 GCTAGC PvuII 1004 CAGCTG CAG^CTG 2879 CAGCTG RcaI 579 TCATGA T^CATGA 1256 TCATGA SspBI 1073 TGTACA T^GTACA 1348 TGTACA StuI 1113 AGGCCT AGG^CCT 1276 AGGCCT StyI 876 CCTTGG C^C[AT][AT]GG 2968 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI BanI BanII BbvI BfaI BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII