##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AhdI AocI ApoI AscI AspEI
BbrPI BclI BfrI BlpI Bpu1102I
BsaAI BseAI BsiWI BspDI BspEI
BspHI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
DraIII DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI MfeI MluI MroI MunI
NcoI NdeI NheI NruI NsiI
NspV PacI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI SbfI
SexAI SfiI SfuI SnaBI SpeI
Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 537 GGTACC G^GTACC
AflIII 552 ACACGT A^C[GA][CT]GT
AgeI 1837 ACCGGT A^CCGGT
Alw44I 2434 GTGCAC G^TGCAC
ApaI 2279 GGGCCC GGGCC^C
ApaLI 2434 GTGCAC G^TGCAC
AseI 3248 ATTAAT AT^TAAT
AsnI 3248 ATTAAT AT^TAAT
Asp700I 1866 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 537 GGTACC G^GTACC
AspI 826 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2919 CCTAGG C^CTAGG
BamHI 3337 GGATCC G^GATCC
BbvCI 2679 GCTGAGG CCTCAGC(-5/-2)
BcgI 1869 CGACTTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 82 AGATCT A^GATCT
BlnI 2919 CCTAGG C^CTAGG
BsaBI 1903 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1264 GAGACG CGTCTC(1/5)
BsmI 1706 GCATTC GAATGC(1/-1)
Bsp1407I 3375 TGTACA T^GTACA
BsrDI 2162 GCAATG GCAATG(2/0)
BsrGI 3375 TGTACA T^GTACA
BssSI 1672 CTCGTG CACGAG(-5/-1)
BstXI 1875 CCATGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 1608 CGGACCG CG^G[AT]CCG
EagI 42 CGGCCG C^GGCCG
EclXI 42 CGGCCG C^GGCCG
Eco52I 42 CGGCCG C^GGCCG
HincII 2441 GTCGAC GT[CT]^[GA]AC
HindII 2441 GTCGAC GT[CT]^[GA]AC
HphI 1770 GGTGA GGTGA(8/7)
KpnI 537 GGTACC GGTAC^C
MamI 1903 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 41 GCGGCCGC GC^GGCCGC
PflFI 826 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1837 ACCGGT A^CCGGT
PshBI 3248 ATTAAT AT^TAAT
RsrII 1608 CGGACCG CG^G[AT]CCG
SacI 54 GAGCTC GAGCT^C
SalI 2441 GTCGAC G^TCGAC
ScaI 3078 AGTACT AGT^ACT
SgrAI 1836 CACCGGTG C[GA]^CCGG[CT]G
SspBI 3375 TGTACA T^GTACA
StuI 2553 AGGCCT AGG^CCT
TfiI 937 GATTC G^A[AT]TC
Tth111I 826 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3323 TCTAGA T^CTAGA
XmnI 1866 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2441 GTCGAC GT^[AC][GT]AC
2925 GTCTAC
AlwNI 116 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1938 CAGCCTCTG
Aor51HI 2291 AGCGCT AGC^GCT
3314 AGCGCT
AviII 1832 TGCGCA TGC^GCA
2531 TGCGCA
BsaI 145 GAGACC GGTCTC(1/5)
1328 GGTCTC
BsaWI 602 ACCGGA [AT]^CCGG[AT]
1837 ACCGGT
BsiEI 42 CGGCCG CG[GA][CT]^CG
196 CGACCG
BspMI 1619 ACCTGC ACCTGC(4/8)
2929 ACCTGC
BsrBI 48 CCGCTC CCGCTC(-3/-3)
3018 CCGCTC
BstAPI 115 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2456 GCACCAGCTGC
Eco47III 2291 AGCGCT AGC^GCT
3314 AGCGCT
EcoNI 681 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
891 CCTTTGGGAGG
FspI 1832 TGCGCA TGC^GCA
2531 TGCGCA
HindIII 1421 AAGCTT A^AGCTT
3175 AAGCTT
KspI 769 CCGCGG CCGC^GG
1333 CCGCGG
MslI 1876 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1887 CACAGACGTG
NaeI 272 GCCGGC GCC^GGC
712 GCCGGC
NgoMIV 272 GCCGGC G^CCGGC
712 GCCGGC
NspI 1778 GCATGC [GA]CATG^[CT]
3107 GCATGC
PaeR7I 51 CTCGAG C^TCGAG
1202 CTCGAG
PflMI 2254 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2299 CCAGGAGGTGG
SacII 769 CCGCGG CCGC^GG
1333 CCGCGG
SmlI 51 CTCGAG C^T[CT][GA]AG
1202 CTCGAG
SphI 1778 GCATGC GCATG^C
3107 GCATGC
Van91I 2254 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2299 CCAGGAGGTGG
XcmI 1176 CCAGGCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2411 CCAATGCCAATCTGG
XhoI 51 CTCGAG C^TCGAG
1202 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI