################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AccIII AclI AflII AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII BbrPI BcgI BciVI BfrI BglII BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspMI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HpaI MamI MluI MroI NdeI NotI NruI NsiI NspV PacI PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 187 GACGTC GACGT^C Acc65I 1494 GGTACC G^GTACC AccI 1599 GTCGAC GT^[AC][GT]AC AcsI 1152 AAATTC [GA]^AATT[CT] AgeI 1823 ACCGGT A^CCGGT Aor51HI 1913 AGCGCT AGC^GCT ApaI 2770 GGGCCC GGGCC^C ApoI 1152 AAATTC [GA]^AATT[CT] Asp718I 1494 GGTACC G^GTACC AvrII 2799 CCTAGG C^CTAGG BalI 1682 TGGCCA TGG^CCA BclI 2034 TGATCA T^GATCA BlnI 2799 CCTAGG C^CTAGG BlpI 988 GCTAAGC GC^T[ACGT]AGC Bpu1102I 988 GCTAAGC GC^T[ACGT]AGC BsaAI 2291 TACGTG [CT]AC^GT[GA] BsaWI 1823 ACCGGT [AT]^CCGG[AT] BsiEI 3114 CGACCG CG[GA][CT]^CG CelII 988 GCTAAGC GC^T[ACGT]AGC DraI 3457 TTTAAA TTT^AAA DrdI 1572 GACTGTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1913 AGCGCT AGC^GCT FbaI 2034 TGATCA T^GATCA HgaI 2816 GACGC GACGC(5/10) KpnI 1494 GGTACC GGTAC^C KspI 830 CCGCGG CCGC^GG MfeI 2560 CAATTG C^AATTG MluNI 1682 TGGCCA TGG^CCA MscI 1682 TGGCCA TGG^CCA MunI 2560 CAATTG C^AATTG NheI 70 GCTAGC G^CTAGC PaeR7I 544 CTCGAG C^TCGAG PflMI 1405 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1823 ACCGGT A^CCGGT PshAI 1592 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 830 CCGCGG CCGC^GG SalI 1599 GTCGAC G^TCGAC SapI 2495 GAAGAGC GCTCTTC(1/4) SexAI 354 ACCAGGT A^CC[AT]GGT Van91I 1405 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 544 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 831 CGCG CG^CG 3021 CGCG AlwNI 1742 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 2244 CAGGAGCTG AocI 871 CCTGAGG CC^T[ACGT]AGG 1836 CCTCAGG BamHI 107 GGATCC G^GATCC 1323 GGATCC BbeI 1342 GGCGCC GGCGC^C 2469 GGCGCC BbvCI 1133 CCTCAGC CCTCAGC(-5/-2) 3024 GCTGAGG BglI 128 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 913 GCCACTCAGGC BsaI 163 GAGACC GGTCTC(1/5) 2551 GAGACC BsrBI 553 CCGCTC CCGCTC(-3/-3) 298 GAGCGG BstAPI 638 GCAATCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3307 GCAGCAGTTGC BstUI 831 CGCG CG^CG 3021 CGCG BstXI 563 CCATGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2805 CCATTGGCCTGG Bsu36I 871 CCTGAGG CC^T[ACGT]AGG 1836 CCTCAGG EaeI 833 CGGCCA [CT]^GGCC[GA] 1682 TGGCCA Eco81I 871 CCTGAGG CC^T[ACGT]AGG 1836 CCTCAGG EcoNI 1354 CCTTTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2719 CCTGCTGCAGG KasI 1342 GGCGCC G^GCGCC 2469 GGCGCC MvnI 831 CGCG CG^CG 3021 CGCG NaeI 128 GCCGGC GCC^GGC 2603 GCCGGC NarI 1342 GGCGCC GG^CGCC 2469 GGCGCC NgoMIV 128 GCCGGC G^CCGGC 2603 GCCGGC PpuMI 866 GGGTCCC [GA]G^G[AT]CC[CT] 1590 AGGACCT PvuII 256 CAGCTG CAG^CTG 1203 CAGCTG SfoI 1342 GGCGCC GGC^GCC 2469 GGCGCC SmaI 1640 CCCGGG CCC^GGG 2079 CCCGGG StuI 1362 AGGCCT AGG^CCT 1612 AGGCCT XmaCI 1640 CCCGGG C^CCGGG 2079 CCCGGG XmaI 1640 CCCGGG C^CCGGG 2079 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII