##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AccIII      AclI        AflII       AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       BbrPI       BcgI        
BciVI       BfrI        BglII       BsaBI       BseAI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspMI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HpaI        MamI        MluI        
MroI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII         187  GACGTC               GACGT^C
Acc65I       1494  GGTACC               G^GTACC
AccI         1599  GTCGAC               GT^[AC][GT]AC
AcsI         1152  AAATTC               [GA]^AATT[CT]
AgeI         1823  ACCGGT               A^CCGGT
Aor51HI      1913  AGCGCT               AGC^GCT
ApaI         2770  GGGCCC               GGGCC^C
ApoI         1152  AAATTC               [GA]^AATT[CT]
Asp718I      1494  GGTACC               G^GTACC
AvrII        2799  CCTAGG               C^CTAGG
BalI         1682  TGGCCA               TGG^CCA
BclI         2034  TGATCA               T^GATCA
BlnI         2799  CCTAGG               C^CTAGG
BlpI          988  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      988  GCTAAGC              GC^T[ACGT]AGC
BsaAI        2291  TACGTG               [CT]AC^GT[GA]
BsaWI        1823  ACCGGT               [AT]^CCGG[AT]
BsiEI        3114  CGACCG               CG[GA][CT]^CG
CelII         988  GCTAAGC              GC^T[ACGT]AGC
DraI         3457  TTTAAA               TTT^AAA
DrdI         1572  GACTGTCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1913  AGCGCT               AGC^GCT
FbaI         2034  TGATCA               T^GATCA
HgaI         2816  GACGC                GACGC(5/10)
KpnI         1494  GGTACC               GGTAC^C
KspI          830  CCGCGG               CCGC^GG
MfeI         2560  CAATTG               C^AATTG
MluNI        1682  TGGCCA               TGG^CCA
MscI         1682  TGGCCA               TGG^CCA
MunI         2560  CAATTG               C^AATTG
NheI           70  GCTAGC               G^CTAGC
PaeR7I        544  CTCGAG               C^TCGAG
PflMI        1405  CCATCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1823  ACCGGT               A^CCGGT
PshAI        1592  GACCTTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         830  CCGCGG               CCGC^GG
SalI         1599  GTCGAC               G^TCGAC
SapI         2495  GAAGAGC              GCTCTTC(1/4)
SexAI         354  ACCAGGT              A^CC[AT]GGT
Van91I       1405  CCATCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          544  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         831  CGCG                 CG^CG
             3021  CGCG                
AlwNI        1742  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2244  CAGGAGCTG           
AocI          871  CCTGAGG              CC^T[ACGT]AGG
             1836  CCTCAGG             
BamHI         107  GGATCC               G^GATCC
             1323  GGATCC              
BbeI         1342  GGCGCC               GGCGC^C
             2469  GGCGCC              
BbvCI        1133  CCTCAGC              CCTCAGC(-5/-2)
             3024  GCTGAGG             
BglI          128  GCCGGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              913  GCCACTCAGGC         
BsaI          163  GAGACC               GGTCTC(1/5)
             2551  GAGACC              
BsrBI         553  CCGCTC               CCGCTC(-3/-3)
              298  GAGCGG              
BstAPI        638  GCAATCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3307  GCAGCAGTTGC         
BstUI         831  CGCG                 CG^CG
             3021  CGCG                
BstXI         563  CCATGCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2805  CCATTGGCCTGG        
Bsu36I        871  CCTGAGG              CC^T[ACGT]AGG
             1836  CCTCAGG             
EaeI          833  CGGCCA               [CT]^GGCC[GA]
             1682  TGGCCA              
Eco81I        871  CCTGAGG              CC^T[ACGT]AGG
             1836  CCTCAGG             
EcoNI        1354  CCTTTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2719  CCTGCTGCAGG         
KasI         1342  GGCGCC               G^GCGCC
             2469  GGCGCC              
MvnI          831  CGCG                 CG^CG
             3021  CGCG                
NaeI          128  GCCGGC               GCC^GGC
             2603  GCCGGC              
NarI         1342  GGCGCC               GG^CGCC
             2469  GGCGCC              
NgoMIV        128  GCCGGC               G^CCGGC
             2603  GCCGGC              
PpuMI         866  GGGTCCC              [GA]G^G[AT]CC[CT]
             1590  AGGACCT             
PvuII         256  CAGCTG               CAG^CTG
             1203  CAGCTG              
SfoI         1342  GGCGCC               GGC^GCC
             2469  GGCGCC              
SmaI         1640  CCCGGG               CCC^GGG
             2079  CCCGGG              
StuI         1362  AGGCCT               AGG^CCT
             1612  AGGCCT              
XmaCI        1640  CCCGGG               C^CCGGG
             2079  CCCGGG              
XmaI         1640  CCCGGG               C^CCGGG
             2079  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
AlwI        AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspLU11I    BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII