##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AflII AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII BbrPI BcgI
BciVI BfrI BglII BsaBI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspMI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HpaI MamI MluI
MroI NdeI NotI NruI NsiI
NspV PacI PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 187 GACGTC GACGT^C
Acc65I 1494 GGTACC G^GTACC
AccI 1599 GTCGAC GT^[AC][GT]AC
AcsI 1152 AAATTC [GA]^AATT[CT]
AgeI 1823 ACCGGT A^CCGGT
Aor51HI 1913 AGCGCT AGC^GCT
ApaI 2770 GGGCCC GGGCC^C
ApoI 1152 AAATTC [GA]^AATT[CT]
Asp718I 1494 GGTACC G^GTACC
AvrII 2799 CCTAGG C^CTAGG
BalI 1682 TGGCCA TGG^CCA
BclI 2034 TGATCA T^GATCA
BlnI 2799 CCTAGG C^CTAGG
BlpI 988 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 988 GCTAAGC GC^T[ACGT]AGC
BsaAI 2291 TACGTG [CT]AC^GT[GA]
BsaWI 1823 ACCGGT [AT]^CCGG[AT]
BsiEI 3114 CGACCG CG[GA][CT]^CG
CelII 988 GCTAAGC GC^T[ACGT]AGC
DraI 3457 TTTAAA TTT^AAA
DrdI 1572 GACTGTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1913 AGCGCT AGC^GCT
FbaI 2034 TGATCA T^GATCA
HgaI 2816 GACGC GACGC(5/10)
KpnI 1494 GGTACC GGTAC^C
KspI 830 CCGCGG CCGC^GG
MfeI 2560 CAATTG C^AATTG
MluNI 1682 TGGCCA TGG^CCA
MscI 1682 TGGCCA TGG^CCA
MunI 2560 CAATTG C^AATTG
NheI 70 GCTAGC G^CTAGC
PaeR7I 544 CTCGAG C^TCGAG
PflMI 1405 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1823 ACCGGT A^CCGGT
PshAI 1592 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 830 CCGCGG CCGC^GG
SalI 1599 GTCGAC G^TCGAC
SapI 2495 GAAGAGC GCTCTTC(1/4)
SexAI 354 ACCAGGT A^CC[AT]GGT
Van91I 1405 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 544 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 831 CGCG CG^CG
3021 CGCG
AlwNI 1742 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2244 CAGGAGCTG
AocI 871 CCTGAGG CC^T[ACGT]AGG
1836 CCTCAGG
BamHI 107 GGATCC G^GATCC
1323 GGATCC
BbeI 1342 GGCGCC GGCGC^C
2469 GGCGCC
BbvCI 1133 CCTCAGC CCTCAGC(-5/-2)
3024 GCTGAGG
BglI 128 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
913 GCCACTCAGGC
BsaI 163 GAGACC GGTCTC(1/5)
2551 GAGACC
BsrBI 553 CCGCTC CCGCTC(-3/-3)
298 GAGCGG
BstAPI 638 GCAATCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3307 GCAGCAGTTGC
BstUI 831 CGCG CG^CG
3021 CGCG
BstXI 563 CCATGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2805 CCATTGGCCTGG
Bsu36I 871 CCTGAGG CC^T[ACGT]AGG
1836 CCTCAGG
EaeI 833 CGGCCA [CT]^GGCC[GA]
1682 TGGCCA
Eco81I 871 CCTGAGG CC^T[ACGT]AGG
1836 CCTCAGG
EcoNI 1354 CCTTTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2719 CCTGCTGCAGG
KasI 1342 GGCGCC G^GCGCC
2469 GGCGCC
MvnI 831 CGCG CG^CG
3021 CGCG
NaeI 128 GCCGGC GCC^GGC
2603 GCCGGC
NarI 1342 GGCGCC GG^CGCC
2469 GGCGCC
NgoMIV 128 GCCGGC G^CCGGC
2603 GCCGGC
PpuMI 866 GGGTCCC [GA]G^G[AT]CC[CT]
1590 AGGACCT
PvuII 256 CAGCTG CAG^CTG
1203 CAGCTG
SfoI 1342 GGCGCC GGC^GCC
2469 GGCGCC
SmaI 1640 CCCGGG CCC^GGG
2079 CCCGGG
StuI 1362 AGGCCT AGG^CCT
1612 AGGCCT
XmaCI 1640 CCCGGG C^CCGGG
2079 CCCGGG
XmaI 1640 CCCGGG C^CCGGG
2079 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII