################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII BamHI BbeI BbrPI BbvCI BclI BfrI BglII BsaHI BsaWI BseAI BsiWI BsmI Bsp1407I BspEI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I FbaI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 2696 GTAGAC GT^[AC][GT]AC AflIII 1656 ACATGT A^C[GA][CT]GT AvaI 188 CTCGGG C^[CT]CG[GA]G BalI 3143 TGGCCA TGG^CCA BanI 377 GGCACC G^G[CT][GA]CC BcgI 2198 GCACTTGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2975 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1007 GCTCAGC GC^T[ACGT]AGC BpmI 197 CTCCAG CTGGAG(16/14) Bpu1102I 1007 GCTCAGC GC^T[ACGT]AGC BsaAI 401 TACGTA [CT]AC^GT[GA] BsaBI 1820 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 114 GAGACC GGTCTC(1/5) BsgI 2169 CTGCAC GTGCAG(16/14) BsiEI 370 CGACCG CG[GA][CT]^CG BsmBI 388 CGTCTC CGTCTC(1/5) BsoBI 188 CTCGGG C^[CT]CG[GA]G BspDI 2206 ATCGAT AT^CGAT BspHI 1911 TCATGA T^CATGA BspLU11I 1656 ACATGT A^CATGT BspMI 708 ACCTGC ACCTGC(4/8) BsrBI 122 GAGCGG CCGCTC(-3/-3) BssSI 119 CACGAG CACGAG(-5/-1) BstEII 146 GGTCACC G^GT[ACGT]ACC BstPI 146 GGTCACC G^GT[ACGT]ACC CelII 1007 GCTCAGC GC^T[ACGT]AGC ClaI 2206 ATCGAT AT^CGAT EaeI 3143 TGGCCA [CT]^GGCC[GA] EcoNI 919 CCTAAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 146 GGTCACC G^GT[ACGT]ACC EcoRV 2153 GATATC GAT^ATC EcoT22I 1721 ATGCAT ATGCA^T HgaI 61 GACGC GACGC(5/10) MamI 1820 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3143 TGGCCA TGG^CCA MscI 3143 TGGCCA TGG^CCA MslI 887 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 2862 CATATG CA^TATG NsiI 1721 ATGCAT ATGCA^T PflMI 2793 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1911 TCATGA T^CATGA SapI 1982 GCTCTTC GCTCTTC(1/4) SnaBI 401 TACGTA TAC^GTA SpeI 692 ACTAGT A^CTAGT SphI 1221 GCATGC GCATG^C TaqI 2207 TCGA T^CGA TthHB8I 2207 TCGA T^CGA Van91I 2793 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 528 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2276 CAGAAACTG AseI 514 ATTAAT AT^TAAT 1556 ATTAAT AsnI 514 ATTAAT AT^TAAT 1556 ATTAAT AvrII 2744 CCTAGG C^CTAGG 3295 CCTAGG BlnI 2744 CCTAGG C^CTAGG 3295 CCTAGG CfoI 88 GCGC GCG^C 1300 GCGC DraII 618 AGGACCC [GA]G^G[ACGT]CC[CT] 642 AGGACCT DrdI 1436 GACTTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1747 GACCAATACGTC EcoO109I 618 AGGACCC [GA]G^G[ACGT]CC[CT] 642 AGGACCT HhaI 88 GCGC GCG^C 1300 GCGC HinP1I 88 GCGC G^CGC 1300 GCGC KspI 20 CCGCGG CCGC^GG 91 CCGCGG NcoI 178 CCATGG C^CATGG 1497 CCATGG PpuMI 618 AGGACCC [GA]G^G[AT]CC[CT] 642 AGGACCT PshBI 514 ATTAAT AT^TAAT 1556 ATTAAT PstI 1226 CTGCAG CTGCA^G 2816 CTGCAG SacII 20 CCGCGG CCGC^GG 91 CCGCGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanII BbsI BbvI BciVI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraI DsaI EarI EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI