##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII BamHI BbeI
BbrPI BbvCI BclI BfrI BglII
BsaHI BsaWI BseAI BsiWI BsmI
Bsp1407I BspEI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FbaI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 2696 GTAGAC GT^[AC][GT]AC
AflIII 1656 ACATGT A^C[GA][CT]GT
AvaI 188 CTCGGG C^[CT]CG[GA]G
BalI 3143 TGGCCA TGG^CCA
BanI 377 GGCACC G^G[CT][GA]CC
BcgI 2198 GCACTTGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2975 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1007 GCTCAGC GC^T[ACGT]AGC
BpmI 197 CTCCAG CTGGAG(16/14)
Bpu1102I 1007 GCTCAGC GC^T[ACGT]AGC
BsaAI 401 TACGTA [CT]AC^GT[GA]
BsaBI 1820 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 114 GAGACC GGTCTC(1/5)
BsgI 2169 CTGCAC GTGCAG(16/14)
BsiEI 370 CGACCG CG[GA][CT]^CG
BsmBI 388 CGTCTC CGTCTC(1/5)
BsoBI 188 CTCGGG C^[CT]CG[GA]G
BspDI 2206 ATCGAT AT^CGAT
BspHI 1911 TCATGA T^CATGA
BspLU11I 1656 ACATGT A^CATGT
BspMI 708 ACCTGC ACCTGC(4/8)
BsrBI 122 GAGCGG CCGCTC(-3/-3)
BssSI 119 CACGAG CACGAG(-5/-1)
BstEII 146 GGTCACC G^GT[ACGT]ACC
BstPI 146 GGTCACC G^GT[ACGT]ACC
CelII 1007 GCTCAGC GC^T[ACGT]AGC
ClaI 2206 ATCGAT AT^CGAT
EaeI 3143 TGGCCA [CT]^GGCC[GA]
EcoNI 919 CCTAAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 146 GGTCACC G^GT[ACGT]ACC
EcoRV 2153 GATATC GAT^ATC
EcoT22I 1721 ATGCAT ATGCA^T
HgaI 61 GACGC GACGC(5/10)
MamI 1820 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3143 TGGCCA TGG^CCA
MscI 3143 TGGCCA TGG^CCA
MslI 887 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 2862 CATATG CA^TATG
NsiI 1721 ATGCAT ATGCA^T
PflMI 2793 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1911 TCATGA T^CATGA
SapI 1982 GCTCTTC GCTCTTC(1/4)
SnaBI 401 TACGTA TAC^GTA
SpeI 692 ACTAGT A^CTAGT
SphI 1221 GCATGC GCATG^C
TaqI 2207 TCGA T^CGA
TthHB8I 2207 TCGA T^CGA
Van91I 2793 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 528 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2276 CAGAAACTG
AseI 514 ATTAAT AT^TAAT
1556 ATTAAT
AsnI 514 ATTAAT AT^TAAT
1556 ATTAAT
AvrII 2744 CCTAGG C^CTAGG
3295 CCTAGG
BlnI 2744 CCTAGG C^CTAGG
3295 CCTAGG
CfoI 88 GCGC GCG^C
1300 GCGC
DraII 618 AGGACCC [GA]G^G[ACGT]CC[CT]
642 AGGACCT
DrdI 1436 GACTTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1747 GACCAATACGTC
EcoO109I 618 AGGACCC [GA]G^G[ACGT]CC[CT]
642 AGGACCT
HhaI 88 GCGC GCG^C
1300 GCGC
HinP1I 88 GCGC G^CGC
1300 GCGC
KspI 20 CCGCGG CCGC^GG
91 CCGCGG
NcoI 178 CCATGG C^CATGG
1497 CCATGG
PpuMI 618 AGGACCC [GA]G^G[AT]CC[CT]
642 AGGACCT
PshBI 514 ATTAAT AT^TAAT
1556 ATTAAT
PstI 1226 CTGCAG CTGCA^G
2816 CTGCAG
SacII 20 CCGCGG CCGC^GG
91 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EarI EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI