################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AflII AflIII AgeI AocI AscI AseI AsnI AvrII BbeI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsiWI BsmI BspDI BspHI BspLU11I BsrBI BsrDI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI Eco81I EcoRI EcoRV EcoT22I FbaI HindIII KasI KspI MamI MfeI MluI MunI NarI NdeI NheI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 2338 GGTACC G^GTACC AccI 1134 GTCTAC GT^[AC][GT]AC AccIII 1181 TCCGGA T^CCGGA AclI 1594 AACGTT AA^CGTT AhdI 1504 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1495 GTGCAC G^TGCAC ApaLI 1495 GTGCAC G^TGCAC Asp700I 1014 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2338 GGTACC G^GTACC AspEI 1504 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2401 TGCGCA TGC^GCA BpmI 3044 CTGGAG CTGGAG(16/14) BsaWI 1181 TCCGGA [AT]^CCGG[AT] BseAI 1181 TCCGGA T^CCGGA Bsp1407I 635 TGTACA T^GTACA BspEI 1181 TCCGGA T^CCGGA BsrGI 635 TGTACA T^GTACA BssSI 1472 CTCGTG CACGAG(-5/-1) DraI 3446 TTTAAA TTT^AAA Eam1105I 1504 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1200 GAAGAG CTCTTC(1/4) EcoNI 1564 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 1459 GGCCGGCC GGCCGG^CC FspI 2401 TGCGCA TGC^GCA KpnI 2338 GGTACC GGTAC^C Ksp632I 1200 GAAGAG CTCTTC(1/4) MroI 1181 TCCGGA T^CCGGA NotI 127 GCGGCCGC GC^GGCCGC NspI 683 GCATGC [GA]CATG^[CT] Psp1406I 1594 AACGTT AA^CGTT SapI 1200 GAAGAGC GCTCTTC(1/4) ScaI 1231 AGTACT AGT^ACT SphI 683 GCATGC GCATG^C SspBI 635 TGTACA T^GTACA StuI 3157 AGGCCT AGG^CCT XbaI 2996 TCTAGA T^CTAGA XmnI 1014 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 2687 GACGCC G[GA]^CG[CT]C 3457 GGCGTC AlwNI 539 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG 2394 CAGATCCTG Aor51HI 5 AGCGCT AGC^GCT 2753 AGCGCT AspI 1935 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2725 GACCCAGTC BamHI 1178 GGATCC G^GATCC 2487 GGATCC BbrPI 1492 CACGTG CAC^GTG 1816 CACGTG BbvCI 480 CCTCAGC CCTCAGC(-5/-2) 2606 GCTGAGG BglII 854 AGATCT A^GATCT 1049 AGATCT BsaAI 1492 CACGTG [CT]AC^GT[GA] 1816 CACGTG BsaHI 2687 GACGCC G[GA]^CG[CT]C 3457 GGCGTC BseRI 373 CTCCTC GAGGAG(10/8) 2492 CTCCTC BsgI 1156 GTGCAG GTGCAG(16/14) 2391 GTGCAG BsiEI 119 CGGCCG CG[GA][CT]^CG 128 CGGCCG BsmBI 2699 CGTCTC CGTCTC(1/5) 1388 GAGACG BspMI 1066 ACCTGC ACCTGC(4/8) 2907 ACCTGC BstAPI 2393 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3176 GCAAAGTGTGC BstEII 2921 GGTCACC G^GT[ACGT]ACC 3060 GGTCACC BstPI 2921 GGTCACC G^GT[ACGT]ACC 3060 GGTCACC EagI 119 CGGCCG C^GGCCG 128 CGGCCG EclXI 119 CGGCCG C^GGCCG 128 CGGCCG Eco47III 5 AGCGCT AGC^GCT 2753 AGCGCT Eco52I 119 CGGCCG C^GGCCG 128 CGGCCG EcoO65I 2921 GGTCACC G^GT[ACGT]ACC 3060 GGTCACC Hin1I 2687 GACGCC G[GA]^CG[CT]C 3457 GGCGTC HpaI 1597 GTTAAC GTT^AAC 3348 GTTAAC NcoI 435 CCATGG C^CATGG 1355 CCATGG PflFI 1935 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2725 GACCCAGTC PmaCI 1492 CACGTG CAC^GTG 1816 CACGTG PmlI 1492 CACGTG CAC^GTG 1816 CACGTG PpuMI 1160 AGGACCT [GA]G^G[AT]CC[CT] 1693 AGGACCT Tth111I 1935 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2725 GACCCAGTC XcmI 1544 CCAAGGGTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2292 CCACTCTTGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BmyI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI