##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AflII AflIII AgeI AocI
AscI AseI AsnI AvrII BbeI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsiWI BsmI BspDI
BspHI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI Eco81I
EcoRI EcoRV EcoT22I FbaI HindIII
KasI KspI MamI MfeI MluI
MunI NarI NdeI NheI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2338 GGTACC G^GTACC
AccI 1134 GTCTAC GT^[AC][GT]AC
AccIII 1181 TCCGGA T^CCGGA
AclI 1594 AACGTT AA^CGTT
AhdI 1504 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1495 GTGCAC G^TGCAC
ApaLI 1495 GTGCAC G^TGCAC
Asp700I 1014 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2338 GGTACC G^GTACC
AspEI 1504 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2401 TGCGCA TGC^GCA
BpmI 3044 CTGGAG CTGGAG(16/14)
BsaWI 1181 TCCGGA [AT]^CCGG[AT]
BseAI 1181 TCCGGA T^CCGGA
Bsp1407I 635 TGTACA T^GTACA
BspEI 1181 TCCGGA T^CCGGA
BsrGI 635 TGTACA T^GTACA
BssSI 1472 CTCGTG CACGAG(-5/-1)
DraI 3446 TTTAAA TTT^AAA
Eam1105I 1504 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1200 GAAGAG CTCTTC(1/4)
EcoNI 1564 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1459 GGCCGGCC GGCCGG^CC
FspI 2401 TGCGCA TGC^GCA
KpnI 2338 GGTACC GGTAC^C
Ksp632I 1200 GAAGAG CTCTTC(1/4)
MroI 1181 TCCGGA T^CCGGA
NotI 127 GCGGCCGC GC^GGCCGC
NspI 683 GCATGC [GA]CATG^[CT]
Psp1406I 1594 AACGTT AA^CGTT
SapI 1200 GAAGAGC GCTCTTC(1/4)
ScaI 1231 AGTACT AGT^ACT
SphI 683 GCATGC GCATG^C
SspBI 635 TGTACA T^GTACA
StuI 3157 AGGCCT AGG^CCT
XbaI 2996 TCTAGA T^CTAGA
XmnI 1014 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 2687 GACGCC G[GA]^CG[CT]C
3457 GGCGTC
AlwNI 539 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
2394 CAGATCCTG
Aor51HI 5 AGCGCT AGC^GCT
2753 AGCGCT
AspI 1935 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2725 GACCCAGTC
BamHI 1178 GGATCC G^GATCC
2487 GGATCC
BbrPI 1492 CACGTG CAC^GTG
1816 CACGTG
BbvCI 480 CCTCAGC CCTCAGC(-5/-2)
2606 GCTGAGG
BglII 854 AGATCT A^GATCT
1049 AGATCT
BsaAI 1492 CACGTG [CT]AC^GT[GA]
1816 CACGTG
BsaHI 2687 GACGCC G[GA]^CG[CT]C
3457 GGCGTC
BseRI 373 CTCCTC GAGGAG(10/8)
2492 CTCCTC
BsgI 1156 GTGCAG GTGCAG(16/14)
2391 GTGCAG
BsiEI 119 CGGCCG CG[GA][CT]^CG
128 CGGCCG
BsmBI 2699 CGTCTC CGTCTC(1/5)
1388 GAGACG
BspMI 1066 ACCTGC ACCTGC(4/8)
2907 ACCTGC
BstAPI 2393 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3176 GCAAAGTGTGC
BstEII 2921 GGTCACC G^GT[ACGT]ACC
3060 GGTCACC
BstPI 2921 GGTCACC G^GT[ACGT]ACC
3060 GGTCACC
EagI 119 CGGCCG C^GGCCG
128 CGGCCG
EclXI 119 CGGCCG C^GGCCG
128 CGGCCG
Eco47III 5 AGCGCT AGC^GCT
2753 AGCGCT
Eco52I 119 CGGCCG C^GGCCG
128 CGGCCG
EcoO65I 2921 GGTCACC G^GT[ACGT]ACC
3060 GGTCACC
Hin1I 2687 GACGCC G[GA]^CG[CT]C
3457 GGCGTC
HpaI 1597 GTTAAC GTT^AAC
3348 GTTAAC
NcoI 435 CCATGG C^CATGG
1355 CCATGG
PflFI 1935 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2725 GACCCAGTC
PmaCI 1492 CACGTG CAC^GTG
1816 CACGTG
PmlI 1492 CACGTG CAC^GTG
1816 CACGTG
PpuMI 1160 AGGACCT [GA]G^G[AT]CC[CT]
1693 AGGACCT
Tth111I 1935 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2725 GACCCAGTC
XcmI 1544 CCAAGGGTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2292 CCACTCTTGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpuAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI