##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI ApoI AscI AseI
AsnI AspEI AvaI AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraIII DrdI DsaI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HgaI HincII HindII
HindIII HinfI HpaI KspI MaeI
MaeII MaeIII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI NaeI NcoI NdeI
NdeII NgoMIV NheI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI Sau3AI
SbfI ScaI SfcI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp45I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 386 GGTACC G^GTACC
AcyI 430 GGCGCC G[GA]^CG[CT]C
Alw44I 49 GTGCAC G^TGCAC
Aor51HI 176 AGCGCT AGC^GCT
ApaI 529 GGGCCC GGGCC^C
ApaLI 49 GTGCAC G^TGCAC
Asp718I 386 GGTACC G^GTACC
AspI 500 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 299 GGACC G^G[AT]CC
AviII 216 TGCGCA TGC^GCA
BbeI 430 GGCGCC GGCGC^C
BciVI 699 GGATAC GTATCC(6/5)
BcnI 338 CCCGG CC^[GC]GG
BpmI 202 CTGGAG CTGGAG(16/14)
BsaHI 430 GGCGCC G[GA]^CG[CT]C
BsaI 498 GAGACC GGTCTC(1/5)
BsiEI 64 CGGCCG CG[GA][CT]^CG
BsmAI 498 GAGAC GTCTC(1/5)
BsrFI 125 ACCGGC [GA]^CCGG[CT]
Cfr10I 125 ACCGGC [GA]^CCGG[CT]
DraI 736 TTTAAA TTT^AAA
EagI 64 CGGCCG C^GGCCG
EarI 6 GAAGAG CTCTTC(1/4)
EclXI 64 CGGCCG C^GGCCG
Eco47III 176 AGCGCT AGC^GCT
Eco52I 64 CGGCCG C^GGCCG
EcoNI 377 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 216 TGCGCA TGC^GCA
Hin1I 430 GGCGCC G[GA]^CG[CT]C
KasI 430 GGCGCC G^GCGCC
KpnI 386 GGTACC GGTAC^C
Ksp632I 6 GAAGAG CTCTTC(1/4)
NarI 430 GGCGCC GG^CGCC
NciI 338 CCCGG CC^[GC]GG
NlaIII 320 CATG CATG^
NotI 63 GCGGCCGC GC^GGCCGC
NspI 319 GCATGC [GA]CATG^[CT]
PflFI 500 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 409 GAGCTC GAGCT^C
SapI 6 GAAGAGC GCTCTTC(1/4)
SexAI 501 ACCAGGT A^CC[AT]GGT
SfoI 430 GGCGCC GGC^GCC
SgrAI 124 CACCGGCG C[GA]^CCGG[CT]G
SmlI 283 CTCAAG C^T[CT][GA]AG
SphI 319 GCATGC GCATG^C
Tth111I 500 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 288 GTAC GT^AC
387 GTAC
Asp700I 6 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
559 GAACCCCTTC
AspHI 49 GTGCAC G[AT]GC[AT]^C
409 GAGCTC
BanI 386 GGTACC G^G[CT][GA]CC
430 GGCGCC
BanII 409 GAGCTC G[GA]GC[CT]^C
529 GGGCCC
BsiHKAI 49 GTGCAC G[AT]GC[AT]^C
409 GAGCTC
BsmFI 380 GGGAC GGGAC(10/14)
471 GTCCC
BspMI 197 ACCTGC ACCTGC(4/8)
293 ACCTGC
HaeII 176 AGCGCT [GA]GCGC^[CT]
430 GGCGCC
HapII 126 CCGG C^CGG
339 CCGG
HpaII 126 CCGG C^CGG
339 CCGG
HphI 142 GGTGA GGTGA(8/7)
32 TCACC
MboII 6 GAAGA GAAGA(8/7)
477 TCTTC
MseI 575 TTAA T^TAA
737 TTAA
MslI 136 CACACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
208 CATCAGCGTG
MspI 126 CCGG C^CGG
339 CCGG
PflMI 111 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
195 CCACCTGCTGG
RsaI 288 GTAC GT^AC
387 GTAC
Tru9I 575 TTAA T^TAA
737 TTAA
Van91I 111 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
195 CCACCTGCTGG
XmnI 6 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
559 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsaJI BsgI BsiYI BslI Bsp1286I
BsrBI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
ItaI MnlI MvaI MvnI MwoI
NlaIV Sau96I ScrFI SfaNI TseI
Tsp509I TspRI