################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AlwNI AocI ApoI AscI AseI AsnI AspEI AvaI AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraIII DrdI DsaI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HgaI HincII HindII HindIII HinfI HpaI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI NaeI NcoI NdeI NdeII NgoMIV NheI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI Sau3AI SbfI ScaI SfcI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 386 GGTACC G^GTACC AcyI 430 GGCGCC G[GA]^CG[CT]C Alw44I 49 GTGCAC G^TGCAC Aor51HI 176 AGCGCT AGC^GCT ApaI 529 GGGCCC GGGCC^C ApaLI 49 GTGCAC G^TGCAC Asp718I 386 GGTACC G^GTACC AspI 500 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 299 GGACC G^G[AT]CC AviII 216 TGCGCA TGC^GCA BbeI 430 GGCGCC GGCGC^C BciVI 699 GGATAC GTATCC(6/5) BcnI 338 CCCGG CC^[GC]GG BpmI 202 CTGGAG CTGGAG(16/14) BsaHI 430 GGCGCC G[GA]^CG[CT]C BsaI 498 GAGACC GGTCTC(1/5) BsiEI 64 CGGCCG CG[GA][CT]^CG BsmAI 498 GAGAC GTCTC(1/5) BsrFI 125 ACCGGC [GA]^CCGG[CT] Cfr10I 125 ACCGGC [GA]^CCGG[CT] DraI 736 TTTAAA TTT^AAA EagI 64 CGGCCG C^GGCCG EarI 6 GAAGAG CTCTTC(1/4) EclXI 64 CGGCCG C^GGCCG Eco47III 176 AGCGCT AGC^GCT Eco52I 64 CGGCCG C^GGCCG EcoNI 377 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 216 TGCGCA TGC^GCA Hin1I 430 GGCGCC G[GA]^CG[CT]C KasI 430 GGCGCC G^GCGCC KpnI 386 GGTACC GGTAC^C Ksp632I 6 GAAGAG CTCTTC(1/4) NarI 430 GGCGCC GG^CGCC NciI 338 CCCGG CC^[GC]GG NlaIII 320 CATG CATG^ NotI 63 GCGGCCGC GC^GGCCGC NspI 319 GCATGC [GA]CATG^[CT] PflFI 500 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 409 GAGCTC GAGCT^C SapI 6 GAAGAGC GCTCTTC(1/4) SexAI 501 ACCAGGT A^CC[AT]GGT SfoI 430 GGCGCC GGC^GCC SgrAI 124 CACCGGCG C[GA]^CCGG[CT]G SmlI 283 CTCAAG C^T[CT][GA]AG SphI 319 GCATGC GCATG^C Tth111I 500 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 288 GTAC GT^AC 387 GTAC Asp700I 6 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 559 GAACCCCTTC AspHI 49 GTGCAC G[AT]GC[AT]^C 409 GAGCTC BanI 386 GGTACC G^G[CT][GA]CC 430 GGCGCC BanII 409 GAGCTC G[GA]GC[CT]^C 529 GGGCCC BsiHKAI 49 GTGCAC G[AT]GC[AT]^C 409 GAGCTC BsmFI 380 GGGAC GGGAC(10/14) 471 GTCCC BspMI 197 ACCTGC ACCTGC(4/8) 293 ACCTGC HaeII 176 AGCGCT [GA]GCGC^[CT] 430 GGCGCC HapII 126 CCGG C^CGG 339 CCGG HpaII 126 CCGG C^CGG 339 CCGG HphI 142 GGTGA GGTGA(8/7) 32 TCACC MboII 6 GAAGA GAAGA(8/7) 477 TCTTC MseI 575 TTAA T^TAA 737 TTAA MslI 136 CACACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 208 CATCAGCGTG MspI 126 CCGG C^CGG 339 CCGG PflMI 111 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 195 CCACCTGCTGG RsaI 288 GTAC GT^AC 387 GTAC Tru9I 575 TTAA T^TAA 737 TTAA Van91I 111 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 195 CCACCTGCTGG XmnI 6 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 559 GAACCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AccII AciI AluI BbvI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BsrBI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I ItaI MnlI MvaI MvnI MwoI NlaIV Sau96I ScrFI SfaNI TseI Tsp509I TspRI