##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbeI
BbrPI BfrI BlnI BsiWI BspDI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
DraI DraIII DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KpnI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacI SalI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2566 GACGTC GACGT^C
AccIII 2532 TCCGGA T^CCGGA
AcsI 27 GAATTT [GA]^AATT[CT]
Alw44I 1364 GTGCAC G^TGCAC
AocI 3876 CCTAAGG CC^T[ACGT]AGG
ApaLI 1364 GTGCAC G^TGCAC
ApoI 27 GAATTT [GA]^AATT[CT]
BbvCI 3811 GCTGAGG CCTCAGC(-5/-2)
BciVI 154 GGATAC GTATCC(6/5)
BclI 2010 TGATCA T^GATCA
BsaBI 2997 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2532 TCCGGA [AT]^CCGG[AT]
BseAI 2532 TCCGGA T^CCGGA
BsiEI 878 CGGCCG CG[GA][CT]^CG
BsmI 1281 GAATGC GAATGC(1/-1)
Bsp1407I 4190 TGTACA T^GTACA
BspEI 2532 TCCGGA T^CCGGA
BspHI 3494 TCATGA T^CATGA
BsrGI 4190 TGTACA T^GTACA
Bsu36I 3876 CCTAAGG CC^T[ACGT]AGG
CpoI 1488 CGGTCCG CG^G[AT]CCG
EagI 878 CGGCCG C^GGCCG
EclXI 878 CGGCCG C^GGCCG
Eco52I 878 CGGCCG C^GGCCG
Eco81I 3876 CCTAAGG CC^T[ACGT]AGG
FbaI 2010 TGATCA T^GATCA
HincII 2270 GTTGAC GT[CT]^[GA]AC
HindII 2270 GTTGAC GT[CT]^[GA]AC
KspI 2316 CCGCGG CCGC^GG
MamI 2997 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2532 TCCGGA T^CCGGA
NcoI 3638 CCATGG C^CATGG
PaeR7I 98 CTCGAG C^TCGAG
RcaI 3494 TCATGA T^CATGA
RsrII 1488 CGGTCCG CG^G[AT]CCG
SacII 2316 CCGCGG CCGC^GG
SapI 807 GCTCTTC GCTCTTC(1/4)
SbfI 2494 CCTGCAGG CCTGCA^GG
ScaI 1839 AGTACT AGT^ACT
SexAI 1371 ACCTGGT A^CC[AT]GGT
Sse8387I 2494 CCTGCAGG CCTGCA^GG
SspBI 4190 TGTACA T^GTACA
TfiI 3606 GAATC G^A[AT]TC
XhoI 98 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 994 GTCTAC GT^[AC][GT]AC
3166 GTCTAC
AspI 484 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3325 GACATTGTC
BamHI 2031 GGATCC G^GATCC
2909 GGATCC
BcgI 100 CGAGAGTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1785 GCATTGTGATCG
BglI 63 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3707 GCCCCTTGGGC
BlpI 1526 GCTCAGC GC^T[ACGT]AGC
3990 GCTCAGC
Bpu1102I 1526 GCTCAGC GC^T[ACGT]AGC
3990 GCTCAGC
BsaI 41 GAGACC GGTCTC(1/5)
2938 GGTCTC
BsrDI 2557 CATTGC GCAATG(2/0)
3209 GCAATG
BssSI 2102 CACGAG CACGAG(-5/-1)
3484 CACGAG
CelII 1526 GCTCAGC GC^T[ACGT]AGC
3990 GCTCAGC
MseI 2621 TTAA T^TAA
4107 TTAA
PflFI 484 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3325 GACATTGTC
PpuMI 141 AGGACCC [GA]G^G[AT]CC[CT]
2837 AGGACCT
PvuII 1829 CAGCTG CAG^CTG
2480 CAGCTG
SmaI 497 CCCGGG CCC^GGG
612 CCCGGG
StuI 94 AGGCCT AGG^CCT
3629 AGGCCT
Tru9I 2621 TTAA T^TAA
4107 TTAA
Tth111I 484 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3325 GACATTGTC
XmaCI 497 CCCGGG C^CCGGG
612 CCCGGG
XmaI 497 CCCGGG C^CCGGG
612 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII