################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbeI BbrPI BfrI BlnI BsiWI BspDI BspLU11I BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI DraI DraIII DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SacI SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 2566 GACGTC GACGT^C AccIII 2532 TCCGGA T^CCGGA AcsI 27 GAATTT [GA]^AATT[CT] Alw44I 1364 GTGCAC G^TGCAC AocI 3876 CCTAAGG CC^T[ACGT]AGG ApaLI 1364 GTGCAC G^TGCAC ApoI 27 GAATTT [GA]^AATT[CT] BbvCI 3811 GCTGAGG CCTCAGC(-5/-2) BciVI 154 GGATAC GTATCC(6/5) BclI 2010 TGATCA T^GATCA BsaBI 2997 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2532 TCCGGA [AT]^CCGG[AT] BseAI 2532 TCCGGA T^CCGGA BsiEI 878 CGGCCG CG[GA][CT]^CG BsmI 1281 GAATGC GAATGC(1/-1) Bsp1407I 4190 TGTACA T^GTACA BspEI 2532 TCCGGA T^CCGGA BspHI 3494 TCATGA T^CATGA BsrGI 4190 TGTACA T^GTACA Bsu36I 3876 CCTAAGG CC^T[ACGT]AGG CpoI 1488 CGGTCCG CG^G[AT]CCG EagI 878 CGGCCG C^GGCCG EclXI 878 CGGCCG C^GGCCG Eco52I 878 CGGCCG C^GGCCG Eco81I 3876 CCTAAGG CC^T[ACGT]AGG FbaI 2010 TGATCA T^GATCA HincII 2270 GTTGAC GT[CT]^[GA]AC HindII 2270 GTTGAC GT[CT]^[GA]AC KspI 2316 CCGCGG CCGC^GG MamI 2997 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2532 TCCGGA T^CCGGA NcoI 3638 CCATGG C^CATGG PaeR7I 98 CTCGAG C^TCGAG RcaI 3494 TCATGA T^CATGA RsrII 1488 CGGTCCG CG^G[AT]CCG SacII 2316 CCGCGG CCGC^GG SapI 807 GCTCTTC GCTCTTC(1/4) SbfI 2494 CCTGCAGG CCTGCA^GG ScaI 1839 AGTACT AGT^ACT SexAI 1371 ACCTGGT A^CC[AT]GGT Sse8387I 2494 CCTGCAGG CCTGCA^GG SspBI 4190 TGTACA T^GTACA TfiI 3606 GAATC G^A[AT]TC XhoI 98 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 994 GTCTAC GT^[AC][GT]AC 3166 GTCTAC AspI 484 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3325 GACATTGTC BamHI 2031 GGATCC G^GATCC 2909 GGATCC BcgI 100 CGAGAGTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1785 GCATTGTGATCG BglI 63 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3707 GCCCCTTGGGC BlpI 1526 GCTCAGC GC^T[ACGT]AGC 3990 GCTCAGC Bpu1102I 1526 GCTCAGC GC^T[ACGT]AGC 3990 GCTCAGC BsaI 41 GAGACC GGTCTC(1/5) 2938 GGTCTC BsrDI 2557 CATTGC GCAATG(2/0) 3209 GCAATG BssSI 2102 CACGAG CACGAG(-5/-1) 3484 CACGAG CelII 1526 GCTCAGC GC^T[ACGT]AGC 3990 GCTCAGC MseI 2621 TTAA T^TAA 4107 TTAA PflFI 484 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3325 GACATTGTC PpuMI 141 AGGACCC [GA]G^G[AT]CC[CT] 2837 AGGACCT PvuII 1829 CAGCTG CAG^CTG 2480 CAGCTG SmaI 497 CCCGGG CCC^GGG 612 CCCGGG StuI 94 AGGCCT AGG^CCT 3629 AGGCCT Tru9I 2621 TTAA T^TAA 4107 TTAA Tth111I 484 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3325 GACATTGTC XmaCI 497 CCCGGG C^CCGGG 612 CCCGGG XmaI 497 CCCGGG C^CCGGG 612 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII