##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AcyI Alw44I Aor51HI
ApaLI AscI AseI AsnI AviII
BbeI BcgI BclI BglI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
BssHII BssSI BstBI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI Hin1I
HpaI KasI KspI MamI MfeI
MunI NaeI NarI NgoMIV NotI
NruI NspV PacI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 1249 TCCGGA T^CCGGA
ApaI 5079 GGGCCC GGGCC^C
AspHI 4219 GAGCAC G[AT]GC[AT]^C
AspI 4057 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1327 CCTAGG C^CTAGG
BalI 199 TGGCCA TGG^CCA
BamHI 1252 GGATCC G^GATCC
BciVI 2865 GGATAC GTATCC(6/5)
BglII 3681 AGATCT A^GATCT
BlnI 1327 CCTAGG C^CTAGG
BlpI 1756 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1756 GCTGAGC GC^T[ACGT]AGC
BseAI 1249 TCCGGA T^CCGGA
BsiHKAI 4219 GAGCAC G[AT]GC[AT]^C
BspEI 1249 TCCGGA T^CCGGA
BspHI 4155 TCATGA T^CATGA
BspLU11I 5040 ACATGT A^CATGT
BsrBI 409 GAGCGG CCGCTC(-3/-3)
Bst1107I 5245 GTATAC GTA^TAC
BstAPI 2389 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5054 GGTCACC G^GT[ACGT]ACC
BstPI 5054 GGTCACC G^GT[ACGT]ACC
BstXI 2057 CCAACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5245 GTATAC GTA^TAC
CelII 1756 GCTGAGC GC^T[ACGT]AGC
EaeI 199 TGGCCA [CT]^GGCC[GA]
EcoO65I 5054 GGTCACC G^GT[ACGT]ACC
MluI 358 ACGCGT A^CGCGT
MluNI 199 TGGCCA TGG^CCA
MroI 1249 TCCGGA T^CCGGA
MscI 199 TGGCCA TGG^CCA
NcoI 599 CCATGG C^CATGG
NdeI 570 CATATG CA^TATG
PflFI 4057 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 864 GTTTAAAC GTTT^AAAC
PshAI 547 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4155 TCATGA T^CATGA
SapI 2174 GCTCTTC GCTCTTC(1/4)
SpeI 4039 ACTAGT A^CTAGT
SphI 4409 GCATGC GCATG^C
SwaI 4647 ATTTAAAT ATTT^AAAT
Tth111I 4057 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AflII 483 CTTAAG C^TTAAG
2371 CTTAAG
AgeI 373 ACCGGT A^CCGGT
517 ACCGGT
AlwNI 2963 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
3128 CAGCTACTG
AocI 586 CCTTAGG CC^T[ACGT]AGG
4298 CCTCAGG
Asp700I 1387 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3550 GAACCAATTC
BanII 4730 GGGCTC G[GA]GC[CT]^C
5079 GGGCCC
BbrPI 2125 CACGTG CAC^GTG
2390 CACGTG
BcnI 115 CCCGG CC^[GC]GG
380 CCCGG
BfrI 483 CTTAAG C^TTAAG
2371 CTTAAG
BsaAI 2125 CACGTG [CT]AC^GT[GA]
2390 CACGTG
Bsp1407I 954 TGTACA T^GTACA
1189 TGTACA
BspDI 3480 ATCGAT AT^CGAT
3754 ATCGAT
BspMI 3667 ACCTGC ACCTGC(4/8)
2550 GCAGGT
BsrDI 3535 CATTGC GCAATG(2/0)
4214 GCAATG
BsrFI 373 ACCGGT [GA]^CCGG[CT]
517 ACCGGT
BsrGI 954 TGTACA T^GTACA
1189 TGTACA
Bsu36I 586 CCTTAGG CC^T[ACGT]AGG
4298 CCTCAGG
Cfr10I 373 ACCGGT [GA]^CCGG[CT]
517 ACCGGT
ClaI 3480 ATCGAT AT^CGAT
3754 ATCGAT
DsaI 599 CCATGG C^C[GA][CT]GG
5050 CCGTGG
Eco81I 586 CCTTAGG CC^T[ACGT]AGG
4298 CCTCAGG
EcoNI 1459 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3996 CCTGAACGAGG
EcoT22I 463 ATGCAT ATGCA^T
1202 ATGCAT
HindIII 2435 AAGCTT A^AGCTT
4357 AAGCTT
NciI 115 CCCGG CC^[GC]GG
380 CCCGG
NheI 4698 GCTAGC G^CTAGC
4823 GCTAGC
NsiI 463 ATGCAT ATGCA^T
1202 ATGCAT
PaeR7I 1049 CTCGAG C^TCGAG
1913 CTCGAG
PflMI 909 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3489 CCAGAAAGTGG
PinAI 373 ACCGGT A^CCGGT
517 ACCGGT
PmaCI 2125 CACGTG CAC^GTG
2390 CACGTG
PmlI 2125 CACGTG CAC^GTG
2390 CACGTG
PpuMI 552 AGGTCCC [GA]G^G[AT]CC[CT]
3664 AGGACCT
PvuII 2413 CAGCTG CAG^CTG
3862 CAGCTG
SspBI 954 TGTACA T^GTACA
1189 TGTACA
StuI 3848 AGGCCT AGG^CCT
4627 AGGCCT
Van91I 909 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3489 CCAGAAAGTGG
XcmI 2935 CCAAGAAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4394 CCAGATTTAAGATGG
XhoI 1049 CTCGAG C^TCGAG
1913 CTCGAG
XmnI 1387 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3550 GAACCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AflIII AhdI AluI AlwI
ApoI Asp718I AspEI AvaI AvaII
BanI BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII Eam1105I EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII