################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AclI AcyI Alw44I Aor51HI ApaLI AscI AseI AsnI AviII BbeI BcgI BclI BglI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI BssHII BssSI BstBI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRV FbaI FseI FspI Hin1I HpaI KasI KspI MamI MfeI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 1249 TCCGGA T^CCGGA ApaI 5079 GGGCCC GGGCC^C AspHI 4219 GAGCAC G[AT]GC[AT]^C AspI 4057 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1327 CCTAGG C^CTAGG BalI 199 TGGCCA TGG^CCA BamHI 1252 GGATCC G^GATCC BciVI 2865 GGATAC GTATCC(6/5) BglII 3681 AGATCT A^GATCT BlnI 1327 CCTAGG C^CTAGG BlpI 1756 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1756 GCTGAGC GC^T[ACGT]AGC BseAI 1249 TCCGGA T^CCGGA BsiHKAI 4219 GAGCAC G[AT]GC[AT]^C BspEI 1249 TCCGGA T^CCGGA BspHI 4155 TCATGA T^CATGA BspLU11I 5040 ACATGT A^CATGT BsrBI 409 GAGCGG CCGCTC(-3/-3) Bst1107I 5245 GTATAC GTA^TAC BstAPI 2389 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 5054 GGTCACC G^GT[ACGT]ACC BstPI 5054 GGTCACC G^GT[ACGT]ACC BstXI 2057 CCAACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 5245 GTATAC GTA^TAC CelII 1756 GCTGAGC GC^T[ACGT]AGC EaeI 199 TGGCCA [CT]^GGCC[GA] EcoO65I 5054 GGTCACC G^GT[ACGT]ACC MluI 358 ACGCGT A^CGCGT MluNI 199 TGGCCA TGG^CCA MroI 1249 TCCGGA T^CCGGA MscI 199 TGGCCA TGG^CCA NcoI 599 CCATGG C^CATGG NdeI 570 CATATG CA^TATG PflFI 4057 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmeI 864 GTTTAAAC GTTT^AAAC PshAI 547 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4155 TCATGA T^CATGA SapI 2174 GCTCTTC GCTCTTC(1/4) SpeI 4039 ACTAGT A^CTAGT SphI 4409 GCATGC GCATG^C SwaI 4647 ATTTAAAT ATTT^AAAT Tth111I 4057 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AflII 483 CTTAAG C^TTAAG 2371 CTTAAG AgeI 373 ACCGGT A^CCGGT 517 ACCGGT AlwNI 2963 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 3128 CAGCTACTG AocI 586 CCTTAGG CC^T[ACGT]AGG 4298 CCTCAGG Asp700I 1387 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3550 GAACCAATTC BanII 4730 GGGCTC G[GA]GC[CT]^C 5079 GGGCCC BbrPI 2125 CACGTG CAC^GTG 2390 CACGTG BcnI 115 CCCGG CC^[GC]GG 380 CCCGG BfrI 483 CTTAAG C^TTAAG 2371 CTTAAG BsaAI 2125 CACGTG [CT]AC^GT[GA] 2390 CACGTG Bsp1407I 954 TGTACA T^GTACA 1189 TGTACA BspDI 3480 ATCGAT AT^CGAT 3754 ATCGAT BspMI 3667 ACCTGC ACCTGC(4/8) 2550 GCAGGT BsrDI 3535 CATTGC GCAATG(2/0) 4214 GCAATG BsrFI 373 ACCGGT [GA]^CCGG[CT] 517 ACCGGT BsrGI 954 TGTACA T^GTACA 1189 TGTACA Bsu36I 586 CCTTAGG CC^T[ACGT]AGG 4298 CCTCAGG Cfr10I 373 ACCGGT [GA]^CCGG[CT] 517 ACCGGT ClaI 3480 ATCGAT AT^CGAT 3754 ATCGAT DsaI 599 CCATGG C^C[GA][CT]GG 5050 CCGTGG Eco81I 586 CCTTAGG CC^T[ACGT]AGG 4298 CCTCAGG EcoNI 1459 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3996 CCTGAACGAGG EcoT22I 463 ATGCAT ATGCA^T 1202 ATGCAT HindIII 2435 AAGCTT A^AGCTT 4357 AAGCTT NciI 115 CCCGG CC^[GC]GG 380 CCCGG NheI 4698 GCTAGC G^CTAGC 4823 GCTAGC NsiI 463 ATGCAT ATGCA^T 1202 ATGCAT PaeR7I 1049 CTCGAG C^TCGAG 1913 CTCGAG PflMI 909 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3489 CCAGAAAGTGG PinAI 373 ACCGGT A^CCGGT 517 ACCGGT PmaCI 2125 CACGTG CAC^GTG 2390 CACGTG PmlI 2125 CACGTG CAC^GTG 2390 CACGTG PpuMI 552 AGGTCCC [GA]G^G[AT]CC[CT] 3664 AGGACCT PvuII 2413 CAGCTG CAG^CTG 3862 CAGCTG SspBI 954 TGTACA T^GTACA 1189 TGTACA StuI 3848 AGGCCT AGG^CCT 4627 AGGCCT Van91I 909 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3489 CCAGAAAGTGG XcmI 2935 CCAAGAAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4394 CCAGATTTAAGATGG XhoI 1049 CTCGAG C^TCGAG 1913 CTCGAG XmnI 1387 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3550 GAACCAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AfaI AflIII AhdI AluI AlwI ApoI Asp718I AspEI AvaI AvaII BanI BbsI BbvCI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII Eam1105I EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII