################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII BcgI BciVI BfrI BglI BsiWI BsmBI BspDI BsrDI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III FseI FspI HindIII KpnI KspI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflIII 1218 ACATGT A^C[GA][CT]GT Alw44I 1224 GTGCAC G^TGCAC AocI 2037 CCTCAGG CC^T[ACGT]AGG ApaI 2537 GGGCCC GGGCC^C ApaLI 1224 GTGCAC G^TGCAC BclI 595 TGATCA T^GATCA BlpI 129 GCTAAGC GC^T[ACGT]AGC Bpu1102I 129 GCTAAGC GC^T[ACGT]AGC BsaBI 455 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 88 CGGCCG CG[GA][CT]^CG Bsp1407I 1501 TGTACA T^GTACA BspLU11I 1218 ACATGT A^CATGT BspMI 3964 GCAGGT ACCTGC(4/8) BsrFI 223 ACCGGC [GA]^CCGG[CT] BsrGI 1501 TGTACA T^GTACA Bsu36I 2037 CCTCAGG CC^T[ACGT]AGG CelII 129 GCTAAGC GC^T[ACGT]AGC Cfr10I 223 ACCGGC [GA]^CCGG[CT] DraIII 2967 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 88 CGGCCG C^GGCCG EclXI 88 CGGCCG C^GGCCG Eco52I 88 CGGCCG C^GGCCG Eco81I 2037 CCTCAGG CC^T[ACGT]AGG EcoNI 538 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3777 GAATTC G^AATTC EcoRV 3795 GATATC GAT^ATC FbaI 595 TGATCA T^GATCA HincII 2585 GTTAAC GT[CT]^[GA]AC HindII 2585 GTTAAC GT[CT]^[GA]AC HpaI 2585 GTTAAC GTT^AAC MamI 455 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PvuII 1714 CAGCTG CAG^CTG SpeI 3678 ACTAGT A^CTAGT SspBI 1501 TGTACA T^GTACA SspI 1887 AATATT AAT^ATT XbaI 781 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AccIII 2004 TCCGGA T^CCGGA 3781 TCCGGA AspI 1112 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1414 GACTCTGTC AvrII 576 CCTAGG C^CTAGG 1569 CCTAGG BalI 716 TGGCCA TGG^CCA 3013 TGGCCA BamHI 28 GGATCC G^GATCC 2093 GGATCC BbeI 244 GGCGCC GGCGC^C 3438 GGCGCC BbrPI 2967 CACGTG CAC^GTG 3570 CACGTG BbvCI 1635 GCTGAGG CCTCAGC(-5/-2) 4011 GCTGAGG BlnI 576 CCTAGG C^CTAGG 1569 CCTAGG BsaAI 2967 CACGTG [CT]AC^GT[GA] 3570 CACGTG BsaWI 2004 TCCGGA [AT]^CCGG[AT] 3781 TCCGGA BseAI 2004 TCCGGA T^CCGGA 3781 TCCGGA BspEI 2004 TCCGGA T^CCGGA 3781 TCCGGA BspHI 1163 TCATGA T^CATGA 3093 TCATGA BsrBI 823 GAGCGG CCGCTC(-3/-3) 1554 GAGCGG BssSI 1782 CTCGTG CACGAG(-5/-1) 2273 CTCGTG BstEII 2885 GGTGACC G^GT[ACGT]ACC 2972 GGTGACC BstPI 2885 GGTGACC G^GT[ACGT]ACC 2972 GGTGACC EcoO65I 2885 GGTGACC G^GT[ACGT]ACC 2972 GGTGACC EcoT22I 1156 ATGCAT ATGCA^T 3610 ATGCAT HaeII 244 GGCGCC [GA]GCGC^[CT] 3438 GGCGCC HgaI 757 GACGC GACGC(5/10) 310 GCGTC KasI 244 GGCGCC G^GCGCC 3438 GGCGCC MluNI 716 TGGCCA TGG^CCA 3013 TGGCCA MroI 2004 TCCGGA T^CCGGA 3781 TCCGGA MscI 716 TGGCCA TGG^CCA 3013 TGGCCA NarI 244 GGCGCC GG^CGCC 3438 GGCGCC NsiI 1156 ATGCAT ATGCA^T 3610 ATGCAT PflFI 1112 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1414 GACTCTGTC PmaCI 2967 CACGTG CAC^GTG 3570 CACGTG PmlI 2967 CACGTG CAC^GTG 3570 CACGTG PstI 1132 CTGCAG CTGCA^G 3962 CTGCAG RcaI 1163 TCATGA T^CATGA 3093 TCATGA SacI 2391 GAGCTC GAGCT^C 3342 GAGCTC SapI 3339 GAAGAGC GCTCTTC(1/4) 3146 GCTCTTC ScaI 682 AGTACT AGT^ACT 1168 AGTACT SfoI 244 GGCGCC GGC^GCC 3438 GGCGCC SmaI 257 CCCGGG CCC^GGG 3721 CCCGGG SphI 701 GCATGC GCATG^C 1454 GCATGC Tth111I 1112 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1414 GACTCTGTC XmaCI 257 CCCGGG C^CCGGG 3721 CCCGGG XmaI 257 CCCGGG C^CCGGG 3721 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI XhoII