##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
BcgI        BciVI       BfrI        BglI        BsiWI       
BsmBI       BspDI       BsrDI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco47III    FseI        
FspI        HindIII     KpnI        KspI        MfeI        
MluI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
Sse8387I    SwaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AflIII       1218  ACATGT               A^C[GA][CT]GT
Alw44I       1224  GTGCAC               G^TGCAC
AocI         2037  CCTCAGG              CC^T[ACGT]AGG
ApaI         2537  GGGCCC               GGGCC^C
ApaLI        1224  GTGCAC               G^TGCAC
BclI          595  TGATCA               T^GATCA
BlpI          129  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      129  GCTAAGC              GC^T[ACGT]AGC
BsaBI         455  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI          88  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1501  TGTACA               T^GTACA
BspLU11I     1218  ACATGT               A^CATGT
BspMI        3964  GCAGGT               ACCTGC(4/8)
BsrFI         223  ACCGGC               [GA]^CCGG[CT]
BsrGI        1501  TGTACA               T^GTACA
Bsu36I       2037  CCTCAGG              CC^T[ACGT]AGG
CelII         129  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        223  ACCGGC               [GA]^CCGG[CT]
DraIII       2967  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           88  CGGCCG               C^GGCCG
EclXI          88  CGGCCG               C^GGCCG
Eco52I         88  CGGCCG               C^GGCCG
Eco81I       2037  CCTCAGG              CC^T[ACGT]AGG
EcoNI         538  CCTGTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        3777  GAATTC               G^AATTC
EcoRV        3795  GATATC               GAT^ATC
FbaI          595  TGATCA               T^GATCA
HincII       2585  GTTAAC               GT[CT]^[GA]AC
HindII       2585  GTTAAC               GT[CT]^[GA]AC
HpaI         2585  GTTAAC               GTT^AAC
MamI          455  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PvuII        1714  CAGCTG               CAG^CTG
SpeI         3678  ACTAGT               A^CTAGT
SspBI        1501  TGTACA               T^GTACA
SspI         1887  AATATT               AAT^ATT
XbaI          781  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   42 enzymes) #
###################################

AccIII       2004  TCCGGA               T^CCGGA
             3781  TCCGGA              
AspI         1112  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1414  GACTCTGTC           
AvrII         576  CCTAGG               C^CTAGG
             1569  CCTAGG              
BalI          716  TGGCCA               TGG^CCA
             3013  TGGCCA              
BamHI          28  GGATCC               G^GATCC
             2093  GGATCC              
BbeI          244  GGCGCC               GGCGC^C
             3438  GGCGCC              
BbrPI        2967  CACGTG               CAC^GTG
             3570  CACGTG              
BbvCI        1635  GCTGAGG              CCTCAGC(-5/-2)
             4011  GCTGAGG             
BlnI          576  CCTAGG               C^CTAGG
             1569  CCTAGG              
BsaAI        2967  CACGTG               [CT]AC^GT[GA]
             3570  CACGTG              
BsaWI        2004  TCCGGA               [AT]^CCGG[AT]
             3781  TCCGGA              
BseAI        2004  TCCGGA               T^CCGGA
             3781  TCCGGA              
BspEI        2004  TCCGGA               T^CCGGA
             3781  TCCGGA              
BspHI        1163  TCATGA               T^CATGA
             3093  TCATGA              
BsrBI         823  GAGCGG               CCGCTC(-3/-3)
             1554  GAGCGG              
BssSI        1782  CTCGTG               CACGAG(-5/-1)
             2273  CTCGTG              
BstEII       2885  GGTGACC              G^GT[ACGT]ACC
             2972  GGTGACC             
BstPI        2885  GGTGACC              G^GT[ACGT]ACC
             2972  GGTGACC             
EcoO65I      2885  GGTGACC              G^GT[ACGT]ACC
             2972  GGTGACC             
EcoT22I      1156  ATGCAT               ATGCA^T
             3610  ATGCAT              
HaeII         244  GGCGCC               [GA]GCGC^[CT]
             3438  GGCGCC              
HgaI          757  GACGC                GACGC(5/10)
              310  GCGTC               
KasI          244  GGCGCC               G^GCGCC
             3438  GGCGCC              
MluNI         716  TGGCCA               TGG^CCA
             3013  TGGCCA              
MroI         2004  TCCGGA               T^CCGGA
             3781  TCCGGA              
MscI          716  TGGCCA               TGG^CCA
             3013  TGGCCA              
NarI          244  GGCGCC               GG^CGCC
             3438  GGCGCC              
NsiI         1156  ATGCAT               ATGCA^T
             3610  ATGCAT              
PflFI        1112  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1414  GACTCTGTC           
PmaCI        2967  CACGTG               CAC^GTG
             3570  CACGTG              
PmlI         2967  CACGTG               CAC^GTG
             3570  CACGTG              
PstI         1132  CTGCAG               CTGCA^G
             3962  CTGCAG              
RcaI         1163  TCATGA               T^CATGA
             3093  TCATGA              
SacI         2391  GAGCTC               GAGCT^C
             3342  GAGCTC              
SapI         3339  GAAGAGC              GCTCTTC(1/4)
             3146  GCTCTTC             
ScaI          682  AGTACT               AGT^ACT
             1168  AGTACT              
SfoI          244  GGCGCC               GGC^GCC
             3438  GGCGCC              
SmaI          257  CCCGGG               CCC^GGG
             3721  CCCGGG              
SphI          701  GCATGC               GCATG^C
             1454  GCATGC              
Tth111I      1112  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1414  GACTCTGTC           
XmaCI         257  CCCGGG               C^CCGGG
             3721  CCCGGG              
XmaI          257  CCCGGG               C^CCGGG
             3721  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PaeR7I      PflMI       PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoI        
XhoII