##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
BcgI BciVI BfrI BglI BsiWI
BsmBI BspDI BsrDI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III FseI
FspI HindIII KpnI KspI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 1218 ACATGT A^C[GA][CT]GT
Alw44I 1224 GTGCAC G^TGCAC
AocI 2037 CCTCAGG CC^T[ACGT]AGG
ApaI 2537 GGGCCC GGGCC^C
ApaLI 1224 GTGCAC G^TGCAC
BclI 595 TGATCA T^GATCA
BlpI 129 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 129 GCTAAGC GC^T[ACGT]AGC
BsaBI 455 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 88 CGGCCG CG[GA][CT]^CG
Bsp1407I 1501 TGTACA T^GTACA
BspLU11I 1218 ACATGT A^CATGT
BspMI 3964 GCAGGT ACCTGC(4/8)
BsrFI 223 ACCGGC [GA]^CCGG[CT]
BsrGI 1501 TGTACA T^GTACA
Bsu36I 2037 CCTCAGG CC^T[ACGT]AGG
CelII 129 GCTAAGC GC^T[ACGT]AGC
Cfr10I 223 ACCGGC [GA]^CCGG[CT]
DraIII 2967 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 88 CGGCCG C^GGCCG
EclXI 88 CGGCCG C^GGCCG
Eco52I 88 CGGCCG C^GGCCG
Eco81I 2037 CCTCAGG CC^T[ACGT]AGG
EcoNI 538 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3777 GAATTC G^AATTC
EcoRV 3795 GATATC GAT^ATC
FbaI 595 TGATCA T^GATCA
HincII 2585 GTTAAC GT[CT]^[GA]AC
HindII 2585 GTTAAC GT[CT]^[GA]AC
HpaI 2585 GTTAAC GTT^AAC
MamI 455 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PvuII 1714 CAGCTG CAG^CTG
SpeI 3678 ACTAGT A^CTAGT
SspBI 1501 TGTACA T^GTACA
SspI 1887 AATATT AAT^ATT
XbaI 781 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 2004 TCCGGA T^CCGGA
3781 TCCGGA
AspI 1112 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1414 GACTCTGTC
AvrII 576 CCTAGG C^CTAGG
1569 CCTAGG
BalI 716 TGGCCA TGG^CCA
3013 TGGCCA
BamHI 28 GGATCC G^GATCC
2093 GGATCC
BbeI 244 GGCGCC GGCGC^C
3438 GGCGCC
BbrPI 2967 CACGTG CAC^GTG
3570 CACGTG
BbvCI 1635 GCTGAGG CCTCAGC(-5/-2)
4011 GCTGAGG
BlnI 576 CCTAGG C^CTAGG
1569 CCTAGG
BsaAI 2967 CACGTG [CT]AC^GT[GA]
3570 CACGTG
BsaWI 2004 TCCGGA [AT]^CCGG[AT]
3781 TCCGGA
BseAI 2004 TCCGGA T^CCGGA
3781 TCCGGA
BspEI 2004 TCCGGA T^CCGGA
3781 TCCGGA
BspHI 1163 TCATGA T^CATGA
3093 TCATGA
BsrBI 823 GAGCGG CCGCTC(-3/-3)
1554 GAGCGG
BssSI 1782 CTCGTG CACGAG(-5/-1)
2273 CTCGTG
BstEII 2885 GGTGACC G^GT[ACGT]ACC
2972 GGTGACC
BstPI 2885 GGTGACC G^GT[ACGT]ACC
2972 GGTGACC
EcoO65I 2885 GGTGACC G^GT[ACGT]ACC
2972 GGTGACC
EcoT22I 1156 ATGCAT ATGCA^T
3610 ATGCAT
HaeII 244 GGCGCC [GA]GCGC^[CT]
3438 GGCGCC
HgaI 757 GACGC GACGC(5/10)
310 GCGTC
KasI 244 GGCGCC G^GCGCC
3438 GGCGCC
MluNI 716 TGGCCA TGG^CCA
3013 TGGCCA
MroI 2004 TCCGGA T^CCGGA
3781 TCCGGA
MscI 716 TGGCCA TGG^CCA
3013 TGGCCA
NarI 244 GGCGCC GG^CGCC
3438 GGCGCC
NsiI 1156 ATGCAT ATGCA^T
3610 ATGCAT
PflFI 1112 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1414 GACTCTGTC
PmaCI 2967 CACGTG CAC^GTG
3570 CACGTG
PmlI 2967 CACGTG CAC^GTG
3570 CACGTG
PstI 1132 CTGCAG CTGCA^G
3962 CTGCAG
RcaI 1163 TCATGA T^CATGA
3093 TCATGA
SacI 2391 GAGCTC GAGCT^C
3342 GAGCTC
SapI 3339 GAAGAGC GCTCTTC(1/4)
3146 GCTCTTC
ScaI 682 AGTACT AGT^ACT
1168 AGTACT
SfoI 244 GGCGCC GGC^GCC
3438 GGCGCC
SmaI 257 CCCGGG CCC^GGG
3721 CCCGGG
SphI 701 GCATGC GCATG^C
1454 GCATGC
Tth111I 1112 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1414 GACTCTGTC
XmaCI 257 CCCGGG C^CCGGG
3721 CCCGGG
XmaI 257 CCCGGG C^CCGGG
3721 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoI
XhoII