##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Aor51HI AscI Asp718I AspI
AvrII BbrPI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaI BsiWI
BspLU11I BssHII Bst1107I BstAPI BstBI
BstZ17I CelII CpoI DraIII Eco47III
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KpnI KspI MluI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 2061 CGCG CG^CG
AhdI 697 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2283 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 697 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 812 GGATCC G^GATCC
BbvCI 1709 CCTCAGC CCTCAGC(-5/-2)
BglII 3263 AGATCT A^GATCT
BsaBI 675 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4703 TGTACA T^GTACA
BspDI 940 ATCGAT AT^CGAT
BsrBI 2540 GAGCGG CCGCTC(-3/-3)
BsrDI 2769 CATTGC GCAATG(2/0)
BsrFI 2236 ACCGGC [GA]^CCGG[CT]
BsrGI 4703 TGTACA T^GTACA
BstEII 880 GGTGACC G^GT[ACGT]ACC
BstPI 880 GGTGACC G^GT[ACGT]ACC
BstUI 2061 CGCG CG^CG
Cfr10I 2236 ACCGGC [GA]^CCGG[CT]
ClaI 940 ATCGAT AT^CGAT
DraI 3579 TTTAAA TTT^AAA
DrdI 34 GACCAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 697 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3460 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 880 GGTGACC G^GT[ACGT]ACC
HindIII 688 AAGCTT A^AGCTT
MamI 675 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3449 CAATTG C^AATTG
MunI 3449 CAATTG C^AATTG
MvnI 2061 CGCG CG^CG
NdeI 3091 CATATG CA^TATG
NheI 3571 GCTAGC G^CTAGC
PaeR7I 2665 CTCGAG C^TCGAG
PshAI 1020 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 845 AGTACT AGT^ACT
SspBI 4703 TGTACA T^GTACA
StuI 132 AGGCCT AGG^CCT
XhoI 2665 CTCGAG C^TCGAG
XmnI 2283 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 677 TCCGGA T^CCGGA
2582 TCCGGA
AcyI 3722 GGCGCC G[GA]^CG[CT]C
4645 GGCGCC
ApaI 1554 GGGCCC GGGCC^C
4444 GGGCCC
AseI 931 ATTAAT AT^TAAT
2125 ATTAAT
AsnI 931 ATTAAT AT^TAAT
2125 ATTAAT
AviII 1286 TGCGCA TGC^GCA
2033 TGCGCA
BbeI 3722 GGCGCC GGCGC^C
4645 GGCGCC
BcgI 3591 CGAGTACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2894 GCAGTGAGGTCG
BglI 2687 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3299 GCCCTGAAGGC
BsaAI 1986 TACGTG [CT]AC^GT[GA]
4392 CACGTA
BsaHI 3722 GGCGCC G[GA]^CG[CT]C
4645 GGCGCC
BsaWI 677 TCCGGA [AT]^CCGG[AT]
2582 TCCGGA
BseAI 677 TCCGGA T^CCGGA
2582 TCCGGA
BsiEI 337 CGGCCG CG[GA][CT]^CG
1936 CGGCCG
BsmI 2298 GCATTC GAATGC(1/-1)
2696 GCATTC
BspEI 677 TCCGGA T^CCGGA
2582 TCCGGA
BspHI 635 TCATGA T^CATGA
794 TCATGA
BssSI 897 CACGAG CACGAG(-5/-1)
143 CTCGTG
EagI 337 CGGCCG C^GGCCG
1936 CGGCCG
EclXI 337 CGGCCG C^GGCCG
1936 CGGCCG
Eco52I 337 CGGCCG C^GGCCG
1936 CGGCCG
EcoRI 616 GAATTC G^AATTC
2977 GAATTC
FspI 1286 TGCGCA TGC^GCA
2033 TGCGCA
Hin1I 3722 GGCGCC G[GA]^CG[CT]C
4645 GGCGCC
KasI 3722 GGCGCC G^GCGCC
4645 GGCGCC
MroI 677 TCCGGA T^CCGGA
2582 TCCGGA
NarI 3722 GGCGCC GG^CGCC
4645 GGCGCC
NspI 2336 GCATGT [GA]CATG^[CT]
4361 GCATGT
PflMI 24 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3408 CCATGCACTGG
PshBI 931 ATTAAT AT^TAAT
2125 ATTAAT
RcaI 635 TCATGA T^CATGA
794 TCATGA
SfoI 3722 GGCGCC GGC^GCC
4645 GGCGCC
Van91I 24 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3408 CCATGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI