################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI Aor51HI AscI Asp718I AspI AvrII BbrPI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaI BsiWI BspLU11I BssHII Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraIII Eco47III EcoRV EcoT22I FbaI FseI HincII HindII HpaI KpnI KspI MluI NaeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 2061 CGCG CG^CG AhdI 697 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2283 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 697 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 812 GGATCC G^GATCC BbvCI 1709 CCTCAGC CCTCAGC(-5/-2) BglII 3263 AGATCT A^GATCT BsaBI 675 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 4703 TGTACA T^GTACA BspDI 940 ATCGAT AT^CGAT BsrBI 2540 GAGCGG CCGCTC(-3/-3) BsrDI 2769 CATTGC GCAATG(2/0) BsrFI 2236 ACCGGC [GA]^CCGG[CT] BsrGI 4703 TGTACA T^GTACA BstEII 880 GGTGACC G^GT[ACGT]ACC BstPI 880 GGTGACC G^GT[ACGT]ACC BstUI 2061 CGCG CG^CG Cfr10I 2236 ACCGGC [GA]^CCGG[CT] ClaI 940 ATCGAT AT^CGAT DraI 3579 TTTAAA TTT^AAA DrdI 34 GACCAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 697 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3460 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 880 GGTGACC G^GT[ACGT]ACC HindIII 688 AAGCTT A^AGCTT MamI 675 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3449 CAATTG C^AATTG MunI 3449 CAATTG C^AATTG MvnI 2061 CGCG CG^CG NdeI 3091 CATATG CA^TATG NheI 3571 GCTAGC G^CTAGC PaeR7I 2665 CTCGAG C^TCGAG PshAI 1020 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 845 AGTACT AGT^ACT SspBI 4703 TGTACA T^GTACA StuI 132 AGGCCT AGG^CCT XhoI 2665 CTCGAG C^TCGAG XmnI 2283 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccIII 677 TCCGGA T^CCGGA 2582 TCCGGA AcyI 3722 GGCGCC G[GA]^CG[CT]C 4645 GGCGCC ApaI 1554 GGGCCC GGGCC^C 4444 GGGCCC AseI 931 ATTAAT AT^TAAT 2125 ATTAAT AsnI 931 ATTAAT AT^TAAT 2125 ATTAAT AviII 1286 TGCGCA TGC^GCA 2033 TGCGCA BbeI 3722 GGCGCC GGCGC^C 4645 GGCGCC BcgI 3591 CGAGTACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2894 GCAGTGAGGTCG BglI 2687 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3299 GCCCTGAAGGC BsaAI 1986 TACGTG [CT]AC^GT[GA] 4392 CACGTA BsaHI 3722 GGCGCC G[GA]^CG[CT]C 4645 GGCGCC BsaWI 677 TCCGGA [AT]^CCGG[AT] 2582 TCCGGA BseAI 677 TCCGGA T^CCGGA 2582 TCCGGA BsiEI 337 CGGCCG CG[GA][CT]^CG 1936 CGGCCG BsmI 2298 GCATTC GAATGC(1/-1) 2696 GCATTC BspEI 677 TCCGGA T^CCGGA 2582 TCCGGA BspHI 635 TCATGA T^CATGA 794 TCATGA BssSI 897 CACGAG CACGAG(-5/-1) 143 CTCGTG EagI 337 CGGCCG C^GGCCG 1936 CGGCCG EclXI 337 CGGCCG C^GGCCG 1936 CGGCCG Eco52I 337 CGGCCG C^GGCCG 1936 CGGCCG EcoRI 616 GAATTC G^AATTC 2977 GAATTC FspI 1286 TGCGCA TGC^GCA 2033 TGCGCA Hin1I 3722 GGCGCC G[GA]^CG[CT]C 4645 GGCGCC KasI 3722 GGCGCC G^GCGCC 4645 GGCGCC MroI 677 TCCGGA T^CCGGA 2582 TCCGGA NarI 3722 GGCGCC GG^CGCC 4645 GGCGCC NspI 2336 GCATGT [GA]CATG^[CT] 4361 GCATGT PflMI 24 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3408 CCATGCACTGG PshBI 931 ATTAAT AT^TAAT 2125 ATTAAT RcaI 635 TCATGA T^CATGA 794 TCATGA SfoI 3722 GGCGCC GGC^GCC 4645 GGCGCC Van91I 24 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3408 CCATGCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI