################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI AhdI Alw44I AlwI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BalI BamHI BbvCI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiWI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BstBI BstEII BstPI BstUI BstXI BstYI CelII Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV FauI FseI HindIII KpnI KspI MflI MluI MluNI MroI MscI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SpeI Sse8387I SspI SwaI Van91I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 3065 GGCGCC G[GA]^CG[CT]C AflII 791 CTTAAG C^TTAAG AflIII 959 ACACGT A^C[GA][CT]GT Aor51HI 3615 AGCGCT AGC^GCT Asp700I 3712 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1774 CCCGAG C^[CT]CG[GA]G BbeI 3065 GGCGCC GGCGC^C BbrPI 960 CACGTG CAC^GTG BclI 4206 TGATCA T^GATCA BcnI 4230 CCGGG CC^[GC]GG BfrI 791 CTTAAG C^TTAAG BpmI 1519 CTGGAG CTGGAG(16/14) BsaBI 4207 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3065 GGCGCC G[GA]^CG[CT]C BsaI 2014 GAGACC GGTCTC(1/5) BsgI 204 CTGCAC GTGCAG(16/14) BsmBI 2411 GAGACG CGTCTC(1/5) BsoBI 1774 CCCGAG C^[CT]CG[GA]G BsrDI 897 GCAATG GCAATG(2/0) Bst1107I 2939 GTATAC GTA^TAC BstAPI 1154 GCACACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 2939 GTATAC GTA^TAC DraII 1824 AGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 4453 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3615 AGCGCT AGC^GCT EcoO109I 1824 AGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 4206 TGATCA T^GATCA HapII 4230 CCGG C^CGG Hin1I 3065 GGCGCC G[GA]^CG[CT]C HpaI 4373 GTTAAC GTT^AAC HpaII 4230 CCGG C^CGG KasI 3065 GGCGCC G^GCGCC MamI 4207 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2456 CAATTG C^AATTG MspI 4230 CCGG C^CGG MunI 2456 CAATTG C^AATTG NarI 3065 GGCGCC GG^CGCC NciI 4230 CCGGG CC^[GC]GG NdeI 1699 CATATG CA^TATG PmaCI 960 CACGTG CAC^GTG PmlI 960 CACGTG CAC^GTG PpuMI 1824 AGGTCCT [GA]G^G[AT]CC[CT] PvuI 1356 CGATCG CGAT^CG SacI 1383 GAGCTC GAGCT^C ScaI 1745 AGTACT AGT^ACT SfoI 3065 GGCGCC GGC^GCC SnaBI 3482 TACGTA TAC^GTA XbaI 57 TCTAGA T^CTAGA XcmI 1668 CCAACTTTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 3712 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 2939 GTATAC GT^[AC][GT]AC 4523 GTAGAC AocI 448 CCTGAGG CC^T[ACGT]AGG 4187 CCTGAGG AviII 96 TGCGCA TGC^GCA 629 TGCGCA BanII 1383 GAGCTC G[GA]GC[CT]^C 4225 GGGCTC BsiEI 1356 CGATCG CG[GA][CT]^CG 3602 CGGTCG BsmI 919 GAATGC GAATGC(1/-1) 3916 GCATTC Bsp1407I 427 TGTACA T^GTACA 4692 TGTACA BsrGI 427 TGTACA T^GTACA 4692 TGTACA BssSI 4200 CTCGTG CACGAG(-5/-1) 4285 CTCGTG Bsu36I 448 CCTGAGG CC^T[ACGT]AGG 4187 CCTGAGG DraI 2124 TTTAAA TTT^AAA 4506 TTTAAA Eco81I 448 CCTGAGG CC^T[ACGT]AGG 4187 CCTGAGG EcoNI 292 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2803 CCTACTTTAGG EcoT22I 1863 ATGCAT ATGCA^T 2541 ATGCAT FspI 96 TGCGCA TGC^GCA 629 TGCGCA NcoI 1686 CCATGG C^CATGG 2009 CCATGG NsiI 1863 ATGCAT ATGCA^T 2541 ATGCAT SapI 560 GAAGAGC GCTCTTC(1/4) 1611 GAAGAGC SspBI 427 TGTACA T^GTACA 4692 TGTACA StuI 607 AGGCCT AGG^CCT 1595 AGGCCT TaqI 644 TCGA T^CGA 1359 TCGA TthHB8I 644 TCGA T^CGA 1359 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AspHI AspI AvaII BanI BbsI BbvI BfaI BmyI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HgaI HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflFI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I