##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I AlwI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvrII BalI BamHI BbvCI
BcgI BciVI BglI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BstBI BstEII BstPI
BstUI BstXI BstYI CelII Cfr10I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV FauI FseI HindIII KpnI
KspI MflI MluI MluNI MroI
MscI MvnI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I RcaI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SpeI Sse8387I SspI SwaI
Van91I XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcyI 3065 GGCGCC G[GA]^CG[CT]C
AflII 791 CTTAAG C^TTAAG
AflIII 959 ACACGT A^C[GA][CT]GT
Aor51HI 3615 AGCGCT AGC^GCT
Asp700I 3712 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1774 CCCGAG C^[CT]CG[GA]G
BbeI 3065 GGCGCC GGCGC^C
BbrPI 960 CACGTG CAC^GTG
BclI 4206 TGATCA T^GATCA
BcnI 4230 CCGGG CC^[GC]GG
BfrI 791 CTTAAG C^TTAAG
BpmI 1519 CTGGAG CTGGAG(16/14)
BsaBI 4207 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3065 GGCGCC G[GA]^CG[CT]C
BsaI 2014 GAGACC GGTCTC(1/5)
BsgI 204 CTGCAC GTGCAG(16/14)
BsmBI 2411 GAGACG CGTCTC(1/5)
BsoBI 1774 CCCGAG C^[CT]CG[GA]G
BsrDI 897 GCAATG GCAATG(2/0)
Bst1107I 2939 GTATAC GTA^TAC
BstAPI 1154 GCACACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2939 GTATAC GTA^TAC
DraII 1824 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 4453 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3615 AGCGCT AGC^GCT
EcoO109I 1824 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 4206 TGATCA T^GATCA
HapII 4230 CCGG C^CGG
Hin1I 3065 GGCGCC G[GA]^CG[CT]C
HpaI 4373 GTTAAC GTT^AAC
HpaII 4230 CCGG C^CGG
KasI 3065 GGCGCC G^GCGCC
MamI 4207 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2456 CAATTG C^AATTG
MspI 4230 CCGG C^CGG
MunI 2456 CAATTG C^AATTG
NarI 3065 GGCGCC GG^CGCC
NciI 4230 CCGGG CC^[GC]GG
NdeI 1699 CATATG CA^TATG
PmaCI 960 CACGTG CAC^GTG
PmlI 960 CACGTG CAC^GTG
PpuMI 1824 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 1356 CGATCG CGAT^CG
SacI 1383 GAGCTC GAGCT^C
ScaI 1745 AGTACT AGT^ACT
SfoI 3065 GGCGCC GGC^GCC
SnaBI 3482 TACGTA TAC^GTA
XbaI 57 TCTAGA T^CTAGA
XcmI 1668 CCAACTTTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3712 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2939 GTATAC GT^[AC][GT]AC
4523 GTAGAC
AocI 448 CCTGAGG CC^T[ACGT]AGG
4187 CCTGAGG
AviII 96 TGCGCA TGC^GCA
629 TGCGCA
BanII 1383 GAGCTC G[GA]GC[CT]^C
4225 GGGCTC
BsiEI 1356 CGATCG CG[GA][CT]^CG
3602 CGGTCG
BsmI 919 GAATGC GAATGC(1/-1)
3916 GCATTC
Bsp1407I 427 TGTACA T^GTACA
4692 TGTACA
BsrGI 427 TGTACA T^GTACA
4692 TGTACA
BssSI 4200 CTCGTG CACGAG(-5/-1)
4285 CTCGTG
Bsu36I 448 CCTGAGG CC^T[ACGT]AGG
4187 CCTGAGG
DraI 2124 TTTAAA TTT^AAA
4506 TTTAAA
Eco81I 448 CCTGAGG CC^T[ACGT]AGG
4187 CCTGAGG
EcoNI 292 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2803 CCTACTTTAGG
EcoT22I 1863 ATGCAT ATGCA^T
2541 ATGCAT
FspI 96 TGCGCA TGC^GCA
629 TGCGCA
NcoI 1686 CCATGG C^CATGG
2009 CCATGG
NsiI 1863 ATGCAT ATGCA^T
2541 ATGCAT
SapI 560 GAAGAGC GCTCTTC(1/4)
1611 GAAGAGC
SspBI 427 TGTACA T^GTACA
4692 TGTACA
StuI 607 AGGCCT AGG^CCT
1595 AGGCCT
TaqI 644 TCGA T^CGA
1359 TCGA
TthHB8I 644 TCGA T^CGA
1359 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AspI AvaII BanI
BbsI BbvI BfaI BmyI BpuAI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HgaI HhaI HinP1I
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflFI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I