##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AspEI AspI AvrII BbeI
BciVI BfrI BglI BglII BlnI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmBI BspDI BspLU11I BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI HgaI Hin1I
KasI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1423 GGTACC G^GTACC
AccIII 286 TCCGGA T^CCGGA
AocI 4005 CCTGAGG CC^T[ACGT]AGG
AseI 1901 ATTAAT AT^TAAT
AsnI 1901 ATTAAT AT^TAAT
Asp718I 1423 GGTACC G^GTACC
AviII 1349 TGCGCA TGC^GCA
BamHI 86 GGATCC G^GATCC
BbsI 369 GAAGAC GAAGAC(2/6)
BbvCI 2757 CCTCAGC CCTCAGC(-5/-2)
BcgI 1662 CGACCTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 369 GAAGAC GAAGAC(2/6)
BsaWI 286 TCCGGA [AT]^CCGG[AT]
BseAI 286 TCCGGA T^CCGGA
BsmFI 3997 GGGAC GGGAC(10/14)
BspEI 286 TCCGGA T^CCGGA
BstXI 837 CCAGAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4005 CCTGAGG CC^T[ACGT]AGG
DraIII 2721 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1655 CCATGG C^C[GA][CT]GG
Eco81I 4005 CCTGAGG CC^T[ACGT]AGG
EcoNI 4001 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1349 TGCGCA TGC^GCA
HpaI 2390 GTTAAC GTT^AAC
KpnI 1423 GGTACC GGTAC^C
MroI 286 TCCGGA T^CCGGA
NcoI 1655 CCATGG C^CATGG
NdeI 1239 CATATG CA^TATG
PflMI 1505 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 528 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1901 ATTAAT AT^TAAT
SacI 2362 GAGCTC GAGCT^C
SalI 3784 GTCGAC G^TCGAC
SapI 1038 GAAGAGC GCTCTTC(1/4)
SexAI 3740 ACCTGGT A^CC[AT]GGT
StuI 140 AGGCCT AGG^CCT
Van91I 1505 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 1470 ACACGT A^C[GA][CT]GT
3420 ACGTGT
Aor51HI 2420 AGCGCT AGC^GCT
2909 AGCGCT
AspHI 1370 GTGCTC G[AT]GC[AT]^C
2362 GAGCTC
AvaI 639 CCCGAG C^[CT]CG[GA]G
912 CCCGAG
BalI 2177 TGGCCA TGG^CCA
2933 TGGCCA
BbrPI 3419 CACGTG CAC^GTG
3449 CACGTG
BclI 1073 TGATCA T^GATCA
1709 TGATCA
BlpI 78 GCTGAGC GC^T[ACGT]AGC
3979 GCTCAGC
Bpu1102I 78 GCTGAGC GC^T[ACGT]AGC
3979 GCTCAGC
BsaAI 3419 CACGTG [CT]AC^GT[GA]
3449 CACGTG
BsiHKAI 1370 GTGCTC G[AT]GC[AT]^C
2362 GAGCTC
BsoBI 639 CCCGAG C^[CT]CG[GA]G
912 CCCGAG
BspHI 3005 TCATGA T^CATGA
4104 TCATGA
BsrBI 553 GAGCGG CCGCTC(-3/-3)
1604 GAGCGG
BsrDI 71 CATTGC GCAATG(2/0)
2197 GCAATG
BssSI 1077 CACGAG CACGAG(-5/-1)
2357 CTCGTG
BstAPI 442 GCACTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1088 GCAGAAATTGC
BstYI 86 GGATCC [GA]^GATC[CT]
2448 GGATCT
CelII 78 GCTGAGC GC^T[ACGT]AGC
3979 GCTCAGC
Eco47III 2420 AGCGCT AGC^GCT
2909 AGCGCT
EcoRI 718 GAATTC G^AATTC
4532 GAATTC
FbaI 1073 TGATCA T^GATCA
1709 TGATCA
HaeII 2420 AGCGCT [GA]GCGC^[CT]
2909 AGCGCT
HincII 2390 GTTAAC GT[CT]^[GA]AC
3784 GTCGAC
HindII 2390 GTTAAC GT[CT]^[GA]AC
3784 GTCGAC
HindIII 2049 AAGCTT A^AGCTT
4304 AAGCTT
MflI 86 GGATCC [GA]^GATC[CT]
2448 GGATCT
MluNI 2177 TGGCCA TGG^CCA
2933 TGGCCA
MscI 2177 TGGCCA TGG^CCA
2933 TGGCCA
NspI 62 ACATGC [GA]CATG^[CT]
2849 GCATGT
PmaCI 3419 CACGTG CAC^GTG
3449 CACGTG
PmlI 3419 CACGTG CAC^GTG
3449 CACGTG
RcaI 3005 TCATGA T^CATGA
4104 TCATGA
SmlI 1143 CTTGAG C^T[CT][GA]AG
3030 CTCAAG
XcmI 240 CCAATGGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
452 CCATGAGGAAAATGG
XhoII 86 GGATCC [GA]^GATC[CT]
2448 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI