##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BlnI BsaAI BsaBI
BsiWI BspDI BspHI BspLU11I BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
DraIII Eam1105I Eco47III EcoNI EcoRV
FspI MamI MluI NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1455 GGTACC G^GTACC
AccIII 1156 TCCGGA T^CCGGA
AocI 1609 CCTCAGG CC^T[ACGT]AGG
ApaI 236 GGGCCC GGGCC^C
Asp718I 1455 GGTACC G^GTACC
BamHI 2547 GGATCC G^GATCC
BciVI 2229 GGATAC GTATCC(6/5)
BglI 165 GCCCACGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 13 AGATCT A^GATCT
BsaI 754 GGTCTC GGTCTC(1/5)
BsaWI 1156 TCCGGA [AT]^CCGG[AT]
BseAI 1156 TCCGGA T^CCGGA
Bsp1407I 2057 TGTACA T^GTACA
BspEI 1156 TCCGGA T^CCGGA
BsrDI 3788 CATTGC GCAATG(2/0)
BsrGI 2057 TGTACA T^GTACA
BstBI 1226 TTCGAA TT^CGAA
BstEII 3896 GGTTACC G^GT[ACGT]ACC
BstPI 3896 GGTTACC G^GT[ACGT]ACC
Bsu36I 1609 CCTCAGG CC^T[ACGT]AGG
DraI 463 TTTAAA TTT^AAA
Eco81I 1609 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3896 GGTTACC G^GT[ACGT]ACC
EcoRI 1482 GAATTC G^AATTC
FseI 226 GGCCGGCC GGCCGG^CC
HpaI 4535 GTTAAC GTT^AAC
KpnI 1455 GGTACC GGTAC^C
MroI 1156 TCCGGA T^CCGGA
NdeI 527 CATATG CA^TATG
NspV 1226 TTCGAA TT^CGAA
PflMI 2683 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2907 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 122 GACGGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 338 CGATCG CGAT^CG
SapI 1858 GAAGAGC GCTCTTC(1/4)
SfuI 1226 TTCGAA TT^CGAA
SgrAI 321 CGCCGGCG C[GA]^CCGG[CT]G
SphI 4096 GCATGC GCATG^C
SspBI 2057 TGTACA T^GTACA
Van91I 2683 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4069 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BbeI 36 GGCGCC GGCGC^C
259 GGCGCC
BclI 2081 TGATCA T^GATCA
2405 TGATCA
BlpI 2668 GCTGAGC GC^T[ACGT]AGC
2722 GCTGAGC
Bpu1102I 2668 GCTGAGC GC^T[ACGT]AGC
2722 GCTGAGC
BsmBI 334 GAGACG CGTCTC(1/5)
1795 GAGACG
BstAPI 3200 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3298 GCAGAGCCTGC
BstXI 263 CCATGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1991 CCAGCACTGTGG
CelII 2668 GCTGAGC GC^T[ACGT]AGC
2722 GCTGAGC
DraII 84 AGGCCCC [GA]G^G[ACGT]CC[CT]
2907 GGGACCT
DrdI 679 GACTGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1510 GACTGTGAAGTC
EagI 49 CGGCCG C^GGCCG
225 CGGCCG
EclXI 49 CGGCCG C^GGCCG
225 CGGCCG
Eco52I 49 CGGCCG C^GGCCG
225 CGGCCG
EcoO109I 84 AGGCCCC [GA]G^G[ACGT]CC[CT]
2907 GGGACCT
FbaI 2081 TGATCA T^GATCA
2405 TGATCA
HgaI 2116 GACGC GACGC(5/10)
1232 GCGTC
KasI 36 GGCGCC G^GCGCC
259 GGCGCC
KspI 66 CCGCGG CCGC^GG
149 CCGCGG
MaeII 1718 ACGT A^CGT
2459 ACGT
MfeI 404 CAATTG C^AATTG
759 CAATTG
MunI 404 CAATTG C^AATTG
759 CAATTG
NarI 36 GGCGCC GG^CGCC
259 GGCGCC
NcoI 474 CCATGG C^CATGG
1790 CCATGG
PaeR7I 1382 CTCGAG C^TCGAG
3572 CTCGAG
SacI 3035 GAGCTC GAGCT^C
3099 GAGCTC
SacII 66 CCGCGG CCGC^GG
149 CCGCGG
SfoI 36 GGCGCC GGC^GCC
259 GGCGCC
StuI 1592 AGGCCT AGG^CCT
4133 AGGCCT
XhoI 1382 CTCGAG C^TCGAG
3572 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AluI Alw44I AlwI
AlwNI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII