################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AcyI AgeI Alw44I ApaLI AscI AseI AsnI Asp700I AviII BamHI BbeI BsaHI BsgI BsiEI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRV EcoT22I FspI Hin1I HindIII HpaI KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PinAI PmeI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 3560 GGTACC G^GTACC AccII 912 CGCG CG^CG AccIII 2832 TCCGGA T^CCGGA AclI 19 AACGTT AA^CGTT AflII 2597 CTTAAG C^TTAAG AflIII 2127 ACGTGT A^C[GA][CT]GT Aor51HI 667 AGCGCT AGC^GCT Asp718I 3560 GGTACC G^GTACC AspI 214 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 3352 CACGTG CAC^GTG BcgI 2075 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2597 CTTAAG C^TTAAG BsaAI 3352 CACGTG [CT]AC^GT[GA] BsaBI 1216 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2832 TCCGGA [AT]^CCGG[AT] BseAI 2832 TCCGGA T^CCGGA BsmBI 785 GAGACG CGTCTC(1/5) BsmI 1980 GAATGC GAATGC(1/-1) BspEI 2832 TCCGGA T^CCGGA BstAPI 338 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 912 CGCG CG^CG DrdI 3685 GACACGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1606 CCATGG C^C[GA][CT]GG Eco47III 667 AGCGCT AGC^GCT FseI 1278 GGCCGGCC GGCCGG^CC HgaI 1469 GCGTC GACGC(5/10) HincII 3719 GTCAAC GT[CT]^[GA]AC HindII 3719 GTCAAC GT[CT]^[GA]AC KpnI 3560 GGTACC GGTAC^C MamI 1216 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2832 TCCGGA T^CCGGA MseI 2598 TTAA T^TAA MvnI 912 CGCG CG^CG NcoI 1606 CCATGG C^CATGG PflFI 214 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3352 CACGTG CAC^GTG PmlI 3352 CACGTG CAC^GTG PshAI 1112 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 19 AACGTT AA^CGTT SpeI 601 ACTAGT A^CTAGT Tru9I 2598 TTAA T^TAA Tth111I 214 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 748 GTCTAC GT^[AC][GT]AC 2717 GTAGAC AfaI 1595 GTAC GT^AC 3561 GTAC AhdI 2765 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3020 GACAGCTCGTC AspEI 2765 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3020 GACAGCTCGTC AvrII 772 CCTAGG C^CTAGG 3514 CCTAGG BclI 1504 TGATCA T^GATCA 3974 TGATCA BglII 2401 AGATCT A^GATCT 3969 AGATCT BlnI 772 CCTAGG C^CTAGG 3514 CCTAGG BsrBI 3778 CCGCTC CCGCTC(-3/-3) 1262 GAGCGG BsrDI 1321 GCAATG GCAATG(2/0) 2175 GCAATG BsrFI 1279 GCCGGC [GA]^CCGG[CT] 3254 GCCGGC BssSI 611 CACGAG CACGAG(-5/-1) 1533 CTCGTG BstEII 766 GGTGACC G^GT[ACGT]ACC 1841 GGTCACC BstPI 766 GGTGACC G^GT[ACGT]ACC 1841 GGTCACC Cfr10I 1279 GCCGGC [GA]^CCGG[CT] 3254 GCCGGC Eam1105I 2765 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3020 GACAGCTCGTC EcoNI 3145 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3416 CCTCCCCGAGG EcoO65I 766 GGTGACC G^GT[ACGT]ACC 1841 GGTCACC EcoRI 1328 GAATTC G^AATTC 1725 GAATTC FbaI 1504 TGATCA T^GATCA 3974 TGATCA HaeII 87 AGCGCC [GA]GCGC^[CT] 667 AGCGCT NaeI 1279 GCCGGC GCC^GGC 3254 GCCGGC NgoMIV 1279 GCCGGC G^CCGGC 3254 GCCGGC PaeR7I 1896 CTCGAG C^TCGAG 3301 CTCGAG PflMI 2818 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3125 CCACCTTCTGG RsaI 1595 GTAC GT^AC 3561 GTAC SapI 1559 GAAGAGC GCTCTTC(1/4) 521 GCTCTTC SmaI 3216 CCCGGG CCC^GGG 3633 CCCGGG Van91I 2818 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3125 CCACCTTCTGG XhoI 1896 CTCGAG C^TCGAG 3301 CTCGAG XmaCI 3216 CCCGGG C^CCGGG 3633 CCCGGG XmaI 3216 CCCGGG C^CCGGG 3633 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII