##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AcyI AgeI Alw44I ApaLI
AscI AseI AsnI Asp700I AviII
BamHI BbeI BsaHI BsgI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco52I EcoRV EcoT22I FspI Hin1I
HindIII HpaI KasI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmeI PshBI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3560 GGTACC G^GTACC
AccII 912 CGCG CG^CG
AccIII 2832 TCCGGA T^CCGGA
AclI 19 AACGTT AA^CGTT
AflII 2597 CTTAAG C^TTAAG
AflIII 2127 ACGTGT A^C[GA][CT]GT
Aor51HI 667 AGCGCT AGC^GCT
Asp718I 3560 GGTACC G^GTACC
AspI 214 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3352 CACGTG CAC^GTG
BcgI 2075 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2597 CTTAAG C^TTAAG
BsaAI 3352 CACGTG [CT]AC^GT[GA]
BsaBI 1216 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2832 TCCGGA [AT]^CCGG[AT]
BseAI 2832 TCCGGA T^CCGGA
BsmBI 785 GAGACG CGTCTC(1/5)
BsmI 1980 GAATGC GAATGC(1/-1)
BspEI 2832 TCCGGA T^CCGGA
BstAPI 338 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 912 CGCG CG^CG
DrdI 3685 GACACGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1606 CCATGG C^C[GA][CT]GG
Eco47III 667 AGCGCT AGC^GCT
FseI 1278 GGCCGGCC GGCCGG^CC
HgaI 1469 GCGTC GACGC(5/10)
HincII 3719 GTCAAC GT[CT]^[GA]AC
HindII 3719 GTCAAC GT[CT]^[GA]AC
KpnI 3560 GGTACC GGTAC^C
MamI 1216 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2832 TCCGGA T^CCGGA
MseI 2598 TTAA T^TAA
MvnI 912 CGCG CG^CG
NcoI 1606 CCATGG C^CATGG
PflFI 214 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3352 CACGTG CAC^GTG
PmlI 3352 CACGTG CAC^GTG
PshAI 1112 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 19 AACGTT AA^CGTT
SpeI 601 ACTAGT A^CTAGT
Tru9I 2598 TTAA T^TAA
Tth111I 214 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 748 GTCTAC GT^[AC][GT]AC
2717 GTAGAC
AfaI 1595 GTAC GT^AC
3561 GTAC
AhdI 2765 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3020 GACAGCTCGTC
AspEI 2765 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3020 GACAGCTCGTC
AvrII 772 CCTAGG C^CTAGG
3514 CCTAGG
BclI 1504 TGATCA T^GATCA
3974 TGATCA
BglII 2401 AGATCT A^GATCT
3969 AGATCT
BlnI 772 CCTAGG C^CTAGG
3514 CCTAGG
BsrBI 3778 CCGCTC CCGCTC(-3/-3)
1262 GAGCGG
BsrDI 1321 GCAATG GCAATG(2/0)
2175 GCAATG
BsrFI 1279 GCCGGC [GA]^CCGG[CT]
3254 GCCGGC
BssSI 611 CACGAG CACGAG(-5/-1)
1533 CTCGTG
BstEII 766 GGTGACC G^GT[ACGT]ACC
1841 GGTCACC
BstPI 766 GGTGACC G^GT[ACGT]ACC
1841 GGTCACC
Cfr10I 1279 GCCGGC [GA]^CCGG[CT]
3254 GCCGGC
Eam1105I 2765 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3020 GACAGCTCGTC
EcoNI 3145 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3416 CCTCCCCGAGG
EcoO65I 766 GGTGACC G^GT[ACGT]ACC
1841 GGTCACC
EcoRI 1328 GAATTC G^AATTC
1725 GAATTC
FbaI 1504 TGATCA T^GATCA
3974 TGATCA
HaeII 87 AGCGCC [GA]GCGC^[CT]
667 AGCGCT
NaeI 1279 GCCGGC GCC^GGC
3254 GCCGGC
NgoMIV 1279 GCCGGC G^CCGGC
3254 GCCGGC
PaeR7I 1896 CTCGAG C^TCGAG
3301 CTCGAG
PflMI 2818 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3125 CCACCTTCTGG
RsaI 1595 GTAC GT^AC
3561 GTAC
SapI 1559 GAAGAGC GCTCTTC(1/4)
521 GCTCTTC
SmaI 3216 CCCGGG CCC^GGG
3633 CCCGGG
Van91I 2818 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3125 CCACCTTCTGG
XhoI 1896 CTCGAG C^TCGAG
3301 CTCGAG
XmaCI 3216 CCCGGG C^CCGGG
3633 CCCGGG
XmaI 3216 CCCGGG C^CCGGG
3633 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII