##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI BbsI
BcgI BclI BfrI BpuAI BseAI
BsiWI Bsp1407I BspDI BspEI BsrFI
BsrGI BssSI BstAPI Cfr10I ClaI
CpoI Eco47III EcoRV EcoT22I FbaI
FseI HindIII HpaI KspI MluI
MroI NaeI NdeI NgoMIV NruI
NsiI PacI PinAI PmeI PshAI
Psp1406I RsrII SacII SbfI SfiI
SgrAI SmaI SnaBI Sse8387I SspBI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
Acc65I 3076 GGTACC G^GTACC
AhdI 2252 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4072 CCTCAGG CC^T[ACGT]AGG
ApaI 3384 GGGCCC GGGCC^C
Asp700I 3836 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3076 GGTACC G^GTACC
AspEI 2252 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2145 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 362 TGCGCA TGC^GCA
AvrII 4309 CCTAGG C^CTAGG
BalI 2220 TGGCCA TGG^CCA
BamHI 2700 GGATCC G^GATCC
BbeI 3067 GGCGCC GGCGC^C
BbrPI 2210 CACGTG CAC^GTG
BlnI 4309 CCTAGG C^CTAGG
BlpI 3391 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3391 GCTCAGC GC^T[ACGT]AGC
BsaAI 2210 CACGTG [CT]AC^GT[GA]
BsaWI 3868 ACCGGA [AT]^CCGG[AT]
BsmBI 3851 CGTCTC CGTCTC(1/5)
BsmI 1736 GAATGC GAATGC(1/-1)
BspHI 2411 TCATGA T^CATGA
BspMI 2460 ACCTGC ACCTGC(4/8)
Bst1107I 876 GTATAC GTA^TAC
BstBI 2679 TTCGAA TT^CGAA
BstEII 1525 GGTCACC G^GT[ACGT]ACC
BstPI 1525 GGTCACC G^GT[ACGT]ACC
BstZ17I 876 GTATAC GTA^TAC
Bsu36I 4072 CCTCAGG CC^T[ACGT]AGG
CelII 3391 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2252 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4072 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1525 GGTCACC G^GT[ACGT]ACC
FspI 362 TGCGCA TGC^GCA
HaeII 3067 GGCGCC [GA]GCGC^[CT]
HincII 1145 GTCGAC GT[CT]^[GA]AC
HindII 1145 GTCGAC GT[CT]^[GA]AC
KasI 3067 GGCGCC G^GCGCC
KpnI 3076 GGTACC GGTAC^C
MfeI 1334 CAATTG C^AATTG
MluNI 2220 TGGCCA TGG^CCA
MscI 2220 TGGCCA TGG^CCA
MunI 1334 CAATTG C^AATTG
NarI 3067 GGCGCC GG^CGCC
NheI 3231 GCTAGC G^CTAGC
NotI 4403 GCGGCCGC GC^GGCCGC
NspV 2679 TTCGAA TT^CGAA
PflFI 2145 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3408 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2210 CACGTG CAC^GTG
PmlI 2210 CACGTG CAC^GTG
PvuI 526 CGATCG CGAT^CG
RcaI 2411 TCATGA T^CATGA
SacI 4264 GAGCTC GAGCT^C
SalI 1145 GTCGAC G^TCGAC
SapI 3836 GAAGAGC GCTCTTC(1/4)
ScaI 2515 AGTACT AGT^ACT
SfoI 3067 GGCGCC GGC^GCC
SfuI 2679 TTCGAA TT^CGAA
SpeI 2779 ACTAGT A^CTAGT
SspI 4549 AATATT AAT^ATT
StuI 793 AGGCCT AGG^CCT
Tth111I 2145 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3408 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1287 TCTAGA T^CTAGA
XmnI 3836 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 2717 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
3409 CAGACCCTG
AseI 2430 ATTAAT AT^TAAT
4161 ATTAAT
AsnI 2430 ATTAAT AT^TAAT
4161 ATTAAT
BbvCI 373 GCTGAGG CCTCAGC(-5/-2)
1461 GCTGAGG
BciVI 4012 GGATAC GTATCC(6/5)
327 GTATCC
BglI 1911 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4406 GCCGCCGGGGC
BsgI 4492 CTGCAC GTGCAG(16/14)
2872 GTGCAG
BspLU11I 2813 ACATGT A^CATGT
3017 ACATGT
BssHII 651 GCGCGC G^CGCGC
3493 GCGCGC
BstXI 4253 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4343 CCAAAGAAGTGG
DraIII 997 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4050 CACATTGTG
DrdI 2929 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3562 GACAACGGAGTC
EagI 2833 CGGCCG C^GGCCG
4404 CGGCCG
EclXI 2833 CGGCCG C^GGCCG
4404 CGGCCG
Eco52I 2833 CGGCCG C^GGCCG
4404 CGGCCG
EcoNI 2465 CCTTATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4060 CCTAGCAAAGG
EcoRI 1723 GAATTC G^AATTC
1789 GAATTC
MspA1I 942 CAGCTG C[AC]G^C[GT]G
3469 CAGCTG
PpuMI 583 AGGACCT [GA]G^G[AT]CC[CT]
2922 GGGACCT
PshBI 2430 ATTAAT AT^TAAT
4161 ATTAAT
PstI 1858 CTGCAG CTGCA^G
3059 CTGCAG
PvuII 942 CAGCTG CAG^CTG
3469 CAGCTG
SwaI 173 ATTTAAAT ATTT^AAAT
4552 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaBI BsaHI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PleI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoI
XhoII